Generation of Active Species in a Large Atmospheric-Pressure Plasma Jet

O’Neill, Feidhlim T.; Twomey, Barry; Law, Victor J.; Milosavljević, Vladimir; Kong, Michael G.; Anghel, Sorin; Dowling, Denis P.

doi:10.1109/tps.2012.2214403

Cited by 29 publications

(16 citation statements)

References 32 publications

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“…The average plasma input power was calculated from Equation over one period of the sine wave using measured current and voltage values:

W = F \int_{t}^{t + T} I false(t false) V false(t false) d t,

where F is frequency, T = 1/ F (period), V is voltage, and I is current . The total discharge power was determined to be 4.33 W. These values are consistent with previous studies of similar nonthermal plasma systems . Given the choice of helium as a working gas and the low level of power applied to the system, it was unsurprising to find that the thermal effects of the plasma were limited.…”

Section: Resultssupporting

confidence: 71%

Plasma deposition of collagen for cell‐culture applications

O'Sullivan

McArdle

O’Reilly

et al. 2019

Plasma Processes & Polymers

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Collagen coating of polystyrene (PS) labware involves a multistep process with high costs and complexity. Nonthermal plasma processes have recently been found to deposit coagulated and biologically functional protein layers in a single process step. A series of investigations were, therefore, conducted to compare plasma‐deposited collagen to an equivalent commercially coated product. Chemical and microscopic analysis suggests that the plasma‐deposited collagen retains the surface morphology and functional chemistry of the traditional collagen coating. Cell‐culture studies using two separate cell lines confirmed that the plasma‐deposited materials replicated the cell viability, proliferation and activity of the traditional collagen surfaces and outperformed uncoated PS plates. The implications of this for the protein coating of plastic labware surfaces are discussed.

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“…The average plasma input power was calculated from Equation over one period of the sine wave using measured current and voltage values:

W = F \int_{t}^{t + T} I false(t false) V false(t false) d t,

Section: Resultssupporting

confidence: 71%

Plasma deposition of collagen for cell‐culture applications

O'Sullivan

McArdle

O’Reilly

et al. 2019

Plasma Processes & Polymers

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“…The gas flow rate was fixed at 10 slm in this study. This APPJ set-up is completed with a C2000 Redline high voltage variable frequency generator, allowing the frequency to be varied from 1-500 kHz [26] .…”

Section: Experimental Section Plasma Sourcementioning

confidence: 99%

Evaluation of the sensitivity of bacterial and yeast cells to cold atmospheric plasma jet treatments

et al. 2015

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The focus of this research was first to determine the influence of the atmospheric plasma drive frequency on the generation of atomic oxygen species and its correlation with the reduction of bacterial load after treatment in vitro. The treatments were carried out using a helium-plasma jet source called PlasmaStream™. The susceptibility of multiple microbial cell lines was investigated in order to compare the response of gram-positive and gram-negative bacteria, as well as a yeast cell line to the atmospheric plasma treatment. It was observed for the source evaluated that at a frequency of 160 kHz, increased levels of oxygen-laden active species (i.e., OH, NO) were generated. At this frequency, the maximum level of bacterial inactivation in vitro was also achieved. Ex vivo studies (using freshly excised porcine skin as a human analog) were also carried out to verify the antibacterial effect of the plasma jet treatment at this optimal operational frequency and to investigate the effect of treatment duration on the reduction of bacterial load. The plasma jet treatment was found to yield a 4 log reduction in bacterial load after 6 min of treatment, with no observable adverse effects on the treatment surface. The gram-negative bacterial cell lines were found to be far more susceptible to the atmospheric plasma treatments than the gram-positive bacteria. Flow cytometric analysis of plasma treated bacterial cells (Escherichia coli) was conducted in order to attain a fundamental understanding of the mode of action of the treatment on bacteria at a cellular level. This study showed that after treatment with the plasma jet, E. coli cells progressed through the following steps of cell death; the inactivation of transport systems, followed by depolarization of the cytoplasmic membrane, and finally permeabilization of the cell wall.

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“…This is comparable to other nonthermal plasma system studies carried out using similar equipment designs. 15 − 17 …”

Section: Resultsmentioning

confidence: 99%

Direct Plasma Deposition of Collagen on 96-Well Polystyrene Plates for Cell Culture

2020

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A cold atmospheric plasma unit was used to deposit a biologic, in this case collagen, onto a surface. A collagen coating was applied to 96-well polystyrene plates at a range of powers to determine the effects of the plasma power on the coating structure and viability. Plasma characterization was carried out using voltage, current, and power measurements. Coating characterization was completed using gravimetric measurement, cell growth, water contact angle, as well as spectroscopic analysis and compared to commercial collagen-coated plates. Cell culture studies were also undertaken. The plasma coating matched the performance of the commercial plate but dramatically reduced production time and cost. This method could allow for automated inline production of collagen-coated plates for cell culture applications.

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Generation of Active Species in a Large Atmospheric-Pressure Plasma Jet

Cited by 29 publications

References 32 publications

Plasma deposition of collagen for cell‐culture applications

Plasma deposition of collagen for cell‐culture applications

Evaluation of the sensitivity of bacterial and yeast cells to cold atmospheric plasma jet treatments

Direct Plasma Deposition of Collagen on 96-Well Polystyrene Plates for Cell Culture

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