Direct Plasma Deposition of Collagen on 96-Well Polystyrene Plates for Cell Culture

Abstract

A cold atmospheric plasma unit was used to deposit a biologic, in this case collagen, onto a surface. A collagen coating was applied to 96-well polystyrene plates at a range of powers to determine the effects of the plasma power on the coating structure and viability. Plasma characterization was carried out using voltage, current, and power measurements. Coating characterization was completed using gravimetric measurement, cell growth, water contact angle, as well as spectroscopic analysis and compared to commercial collagen-coated plates. Cell culture studies were also undertaken. The plasma coating matched the performance of the commercial plate but dramatically reduced production time and cost. This method could allow for automated inline production of collagen-coated plates for cell culture applications.

Plasma‐assisted surface engineering for value added in starch bioplastics: A study on enhanced surface properties and natural dye immobilization

“…[12][13][14][15] The potential of plasma technology has also been increasing across different material fields and applications. [16][17][18][19] This research stands at the forefront of combining two burgeoning fields: bioplastics and natural dye application. While the potential of bioplastics has been increasingly recognized, their coloration with natural dyes represents a novel approach that could revolutionize the industry.…”

“…Plasma treatment has emerged as a solution, enhancing the bonding strength between dyes and substrates without using solvents and thus being environmentally friendly 12–15 . The potential of plasma technology has also been increasing across different material fields and applications 16–19 …”

Understanding the impact of bioactive coating materials for human mesenchymal stromal cells and implications for manufacturing

“…Despite the complexity and challenges this presents, these methods have become well established and represent the standard surfaces for growing hMSCs on polystyrene surfaces. However, these methods often struggle to provide adequate coatings on less reactive plastics such as COP (O’Sullivan et al 2020c ).…”

Plasma Activation of Microplates Optimized for One-Step Reagent-Free Immobilization of DNA and Protein

“…The density of active groups incorporated during plasma treatment in these approaches is also limited by gas mixtures required to produce a mechanically robust surface coating. Another approach involves the direct deposition of nebulized protein, such as collagen, on microplates by dielectric barrier discharge plasma . However, this method requires large quantities of protein and not all biomolecules incorporated into the coating are accessible on the surface.…”

“…Another approach involves the direct deposition of nebulized protein, such as collagen, on microplates by dielectric barrier discharge plasma. 35 However, this method requires large quantities of protein and not all biomolecules incorporated into the coating are accessible on the surface. PIII and PAC treatments for direct activation of microplates for biomolecule immobilization have the potential to mitigate these limitations.…”