Generation of a nucleosome-free promoter region in SV40 does not require T-Antigen binding to site I

Histone Hyperacetylation in the Coding Region of Chromatin Undergoing Transcription in SV40 Minichromosomes Is a Dynamic Process Regulated Directly by the Presence of RNA Polymerase II

“…As is apparent from Figure 2, hyperacetylated H4 and H3 were found on the early, late and promoter regions of the genome co-localized with the RNAPII (from ReChIP lanes 6,7,13,14,20,21) as well as independent of the RNAPII (from ISFIP lanes 4,5,11,12,18,19). In contrast, when the chromatin fragments from the ISFIP were subjected to a ChIP analysis with antibody to RNAPII, no product was generated by PCR amplification (lanes 3,10,17) indicating that all of the fragments containing RNAPII remained bound to the protein A agarose during the immune selection step and subsequent chromatin fragmentation.…”

“…Sub-confluent monolayer of cells were infected with either the wild-type 776 virus or mutant cs1085 virus as previously described (18) . The virus was allowed to adsorb onto the cells for 30 min.…”

Virion-mediated transfer of SV40 epigenetic information

“…18,19 This deletion results in an over-production of early mRNA and protein. We have previously exploited this aspect of cs1085 to investigate nucleosome phasing 20 and RNA polymerase II occupancy in transcribing SV40 minichromosomes.…”

Genome Sequence of Canine Polyomavirus in Respiratory Secretions of Dogs with Pneumonia of Unknown Etiology

“…Phylogenetic analysis based on the large TAg indicates that DogPyV-1 is a new species in the genus Betapolyomavirus (2). BLASTp analysis of the DogPyV-1 LT antigen showed the most closely related large TAg belonged to different primate polyomaviruses, including Macaca mulatta polyomavirus 1 (3), Cercopithecus erythrotis polyomavirus 1 (4), Simian virus 12 (5), Vervet monkey polyomavirus 2 (6), human polyomavirus 1 (7), Anubis baboon polyomavius (GenBank accession no. KJ577598.1) and Yellow baboon polyomavirus 2 (6) with identities of 53 to 56% over 89 to 96% of the length of their LT proteins.…”