Generation of a Human iPSC-Based Blood-Brain Barrier Chip

Jagadeesan, Srikanth; Workman, Michael J.; Herland, Anna; Svendsen, Clive N.; Vatine, Gad D.

doi:10.3791/60925

Cited by 21 publications

(23 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the most noteworthy recent advances in iPSCderived models of the BBB has come in the generation of bioengineered microfluidic organ-chip-based models [54][55][56]. These microphysiological systems typically incorporate iBMECs into hydrogels [57] or polydimethylsiloxane (PDMS)-based devices [58] that attempt to recreate the anatomical, physiological and mechanical forces that cells experience in vivo. Much of this work has been reviewed previously [59], and thus, we focus on several recent advances that have extended progress in this area.…”

Section: Ipsc-derived Microfluidic Chip Models Of the Bbbmentioning

confidence: 99%

Recent advances in human iPSC-derived models of the blood–brain barrier

Workman

Svendsen

2020

Fluids Barriers CNS

Self Cite

View full text Add to dashboard Cite

The blood-brain barrier (BBB) is a critical component of the central nervous system that protects neurons and other cells of the brain parenchyma from potentially harmful substances found in peripheral circulation. Gaining a thorough understanding of the development and function of the human BBB has been hindered by a lack of relevant models given significant species differences and limited access to in vivo tissue. However, advances in induced pluripotent stem cell (iPSC) and organ-chip technologies now allow us to improve our knowledge of the human BBB in both health and disease. This review focuses on the recent progress in modeling the BBB in vitro using human iPSCs.

show abstract

Section: Ipsc-derived Microfluidic Chip Models Of the Bbbmentioning

confidence: 99%

Recent advances in human iPSC-derived models of the blood–brain barrier

Workman

Svendsen

2020

Fluids Barriers CNS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The limitation makes iPSC-derived neurons difficult to analyze in unified experimental conditions. Nonetheless, iPSC-derived neurons have been widely used in designing a 3D brain-on-a-chip models [ 34 , 35 , 36 , 37 ], neurotoxicity testing [ 38 ], and disease modeling [ 39 , 40 ], owing to its advantages for various cellular populations and genetic conditions ( Figure 2 ). These models are expected to be used to develop personalized medicines (notably, for sporadic ND patients).…”

Section: Neural Cells For Neural Microphysiological System (Neuralmentioning

confidence: 99%

Microphysiological Systems for Neurodegenerative Diseases in Central Nervous System

Bae

Jang

et al. 2020

Micromachines

View full text Add to dashboard Cite

Neurodegenerative diseases are among the most severe problems in aging societies. Various conventional experimental models, including 2D and animal models, have been used to investigate the pathogenesis of (and therapeutic mechanisms for) neurodegenerative diseases. However, the physiological gap between humans and the current models remains a hurdle to determining the complexity of an irreversible dysfunction in a neurodegenerative disease. Therefore, preclinical research requires advanced experimental models, i.e., those more physiologically relevant to the native nervous system, to bridge the gap between preclinical stages and patients. The neural microphysiological system (neural MPS) has emerged as an approach to summarizing the anatomical, biochemical, and pathological physiology of the nervous system for investigation of neurodegenerative diseases. This review introduces the components (such as cells and materials) and fabrication methods for designing a neural MPS. Moreover, the review discusses future perspectives for improving the physiological relevance to native neural systems.

show abstract

“…The platform was also used to develop a patient personalized model for neurological disorders. [ 58 ] In another recent study, human embryonic stem cells were employed to build a brain on‐chip platform to study the effect of prenatal cannabis exposure (Figure 5E). A total of 169 organoids were successfully created without fusion in an area of 3.5 × 3.5 cm 2 .…”

Section: High‐throughput Biomaterials Platforms In Tissue Engineering mentioning

confidence: 99%

Recent Advances in Biomaterial‐Based High‐Throughput Platforms

Sarkar

Kumar

2020

Biotechnology Journal

View full text Add to dashboard Cite

High‐throughput systems allow screening and analysis of large number of samples simultaneously under same conditions. Over recent years, high‐throughput systems have found applications in fields other than drug discovery like bioprocess industries, pollutant detection, material microarrays, etc. With the introduction of materials in such HT platforms, the screening system has been enabled for solid phases apart from conventional solution phase. The use of biomaterials has further facilitated cell‐based assays in such platforms. Here, the authors have focused on the recent developments in biomaterial‐based platforms including the fabricationusing contact and non‐contact methods and utilization of such platforms for discovery of novel biomaterials exploiting interaction of biological entities with surface and bulk properties. Finally, the authors have elaborated on the application of the biomaterial‐based high‐throughput platforms in tissue engineering and regenerative medicine, cancer and stem cell studies. The studies show encouraging applications of biomaterial microarrays. However, success in clinical applicability still seems to be a far off task majorly due to absence of robust characterization and analysis techniques. Extensive focus is required for developing personalized medicine, analytical tools and storage/shelf‐life of cell laden microarrays.

show abstract

Generation of a Human iPSC-Based Blood-Brain Barrier Chip

Cited by 21 publications

References 0 publications

Recent advances in human iPSC-derived models of the blood–brain barrier

Recent advances in human iPSC-derived models of the blood–brain barrier

Microphysiological Systems for Neurodegenerative Diseases in Central Nervous System

Recent Advances in Biomaterial‐Based High‐Throughput Platforms

Contact Info

Product

Resources

About