Generation of a bioengineered autologous bone substitute for palate repair: anin vivostudy in laboratory animals

Abstract

We carried out an in vivo study to evaluate the potential usefulness of a novel bioengineered bone substitute for the repair of palate defects in laboratory rabbits, using tissue-engineering methods. Our results showed that the use of a bioengineered bone substitute was associated with more symmetrical palate growth as compared to the controls, and the length and height of the palate were very similar on both sides of the palate, with differences from negative controls 4 months after artificial bone grafting for bone length. The histological analysis revealed that the regenerated bone was well organized and expressed osteocalcin. In contrast, bone corresponding to control animals without tissue grafting was immature, with areas of osteoid tissue and remodelling, as determined by MMP-14 expression. These results suggest that bone substitutes may be a useful strategy to induce the formation of a well-structured palate bone, which could prevent the growth alterations found in cleft palate patients. This opens a door to a future clinical application of these bone substitutes. Copyright © 2015 John Wiley & Sons, Ltd.

Usefulness of a Nanostructured Fibrin-Agarose Bone Substitute in a Model of Severely Critical Mandible Bone Defect

“…Although these results are preliminary, our results suggest that this technology could be useful in cases with severe morphological and structural alterations with loss of mandibular bone. Even though they used a different animal model, previous works carried out by our research group demonstrated that fibrin-agarose-based bioartificial tissues could contribute to induce harmonic development of maxillofacial structures in rabbits with a palate bone defect [26,38].…”

“…Immediately after euthanasia, animals were analyzed using a Pointnix Point 3D Combi 500 CT scanner, as previously reported [26]. For this purpose, the head of each animal was placed and fixed on the analysis surface, and high-resolution images were obtained from all cranial structures.…”

Tissue engineering, university and health system. History of an experience

“…Fruto de esta actividad investigadora ha sido la generación de distintos tejidos artificiales como córnea (14) , piel (15,16), mucosa oral (17), nervio periférico (18), paladar (19), cartílago (20), etc. utilizando distintos tipos celulares, biomateriales , procesos de biofabricación (21, 22, 23) y protocolos de control de calidad de los que se ha dado cuenta en publicaciones científicas (24,25,26,27,28,29,30).…”

Surgical Creation of Cleft-Like Palatal Defects in New Zealand Rabbits (A Pilot Study)

“…Some studies discussed the creation of palatal defects in rabbits; such as El Bokle et al in 1993 (24) and Liceras-Liceras et al in 2017 (25), but those did not include the mid palatal suture. Mostafa etal.…”