Genetic effects on fiber quality parameters in six basic populations (P1, P2, F1, F2, BC1 and BC2) of crosses viz. TARZAN-1× CIM-602 and A-555×FH-114 were investigated through GMA. The outcomes of the current study envisaged that additive and dominant alongside non-allelic interactions assumed a significant role for the attributes like fiber length and fiber maturity in both crosses, however, additive genetic actions [d] were observed to be imperative for fiber uniformity, fineness and bundle strength. Epistatic gene interactions were present almost in all the quality traits in one or other cross, whereas linkage was prominently observed in bundle strength and fineness. All the fiber traits showed high genetic variance and genetic advance except fiber length where genetic gain was relatively low under normal and water stressed conditions. Inverse indication of [h] and [i] anticipated duplicate genes in the legacy of fiber fineness and length that will bring plant breeders in trouble during selection. Hence, heterosis breeding would be a better choice because complementary gene interaction was absent in all quality traits.