Generation and Evaluation of High β-Glucan Producing Mutant Strains ofSparassis crispa

Abstract

A chemical mutagenesis technique was employed for development of mutant strains of Sparassis crispa targeting the shortened cultivation time and the high β-glucan content. The homogenized mycelial fragments of S. crispa IUM4010 strain were treated with 0.2 vol% methyl methanesulfonate, an alkylating agent, yielding 199 mutant strains. Subsequent screening in terms of growth and β-glucan content yielded two mutant strains, B4 and S7. Both mutants exhibited a significant increase in β-glucan productivity by producing 0.254 and 0.236 mg soluble β-glucan/mg dry cell weight for the B4 and S7 strains, respectively, whereas the wild type strain produced 0.102 mg soluble β-glucan/mg dry cell weight. The results demonstrate the usefulness of chemical mutagenesis for generation of mutant mushroom strains.

Alginate-Derived Elicitors Enhance β-Glucan Content and Antioxidant Activities in Culinary and Medicinal Mushroom, Sparassis latifolia

“…Sparassis species are widely used medicinal mushrooms in traditional Chinese medicine. However, despite its commercial potential, cultivation of S. latifolia has been limited to a few high-tech commercial farms because of its slow mycelial propagation into solid medium [42]. In addition, its slow rate of growth is a major obstacle to the employment of S. latifolia as a producer of β-glucan.…”

Морфолого-культуральні властивості рідкісного лікарського гриба Sparassis crispa (Sparassidaceae, Polyporales)

“…Сьогодні цей вид розглядають як перспективний об'єкт для біотехнології отримання не лише їстівних плодових тіл, а і як джерело β -глюканів у промислових масштабах (Hadara et al, 2003;Kim et al, 2013;Takashi, 2013;Yang et al, 2014).…”

Isolation and Characterization of Monokaryotic Strains ofLentinula edodesShowing Higher Fruiting Rate and Better Fruiting Body Production

“…Sixteen and 15 alleles at loci A and B, respectively, were reported from 12 strains of Pleurotus eryngii , suggesting that P. eryngii can have at least 240 (15 × 16) different compatible mating pairs when different pairs of monokaryotic strains are used [ 3 ]. Random mutagenesis by chemical or physical means does not change the mating rate, but can be applied to enhance the diversity of the genetic pool [ 13 , 17 , 18 ]. A temperature-adapted strain of P. ostreatus and a high β-glucan producing Hypsizygus marmoreus were recently developed using a combination of mycelial mating and chemical mutagenesis [ 17 , 19 ].…”

“…Random mutagenesis by chemical or physical means does not change the mating rate, but can be applied to enhance the diversity of the genetic pool [ 13 , 17 , 18 ]. A temperature-adapted strain of P. ostreatus and a high β-glucan producing Hypsizygus marmoreus were recently developed using a combination of mycelial mating and chemical mutagenesis [ 17 , 19 ]. Therefore, diversification of the genetic pool by either vast collection from the wild or physicochemical mutagenesis is necessary for generation of new strains and varieties of mushrooms.…”