Generation and analysis of expressed sequence tags from poplar (Populus alba×P. tremula var. glandulosa) suspension cells

Lee, Hyoshin; Lee, Jae-Soon; Noh, Eun Woon; Bae, Eun-Kyung; Choi, Young‐Im; Han, Mu-Seok

doi:10.1016/j.plantsci.2005.07.013

Cited by 19 publications

(13 citation statements)

References 28 publications

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“…This contrasts to our experience with other poplar genes, where genes were frequently up-regulated in suspension culture (Lee et al, 2005). Thus, we monitored the changes in the expression of PatPIP1 in the growing cells in the suspension culture as previously described by Lee et al (2005). Although the PatPIP1 transcript was undetectable at the lag phase (4 days), the expression level dramatically increased at 8 days, and remained fairly constant until it decreased at the middle exponential growth phase (Fig.…”

Section: Expression Analysis Of Patpip1 During Cell Growthmentioning

confidence: 92%

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Drought, salt and wounding stress induce the expression of the plasma membrane intrinsic protein 1 gene in poplar (Populus alba×P. tremula var. glandulosa)

Bae¹,

Lee

et al. 2011

Gene

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Section: Expression Analysis Of Patpip1 During Cell Growthmentioning

confidence: 92%

Section: Expression Analysis Of Patpip1 During Cell Growthmentioning

confidence: 98%

Drought, salt and wounding stress induce the expression of the plasma membrane intrinsic protein 1 gene in poplar (Populus alba×P. tremula var. glandulosa)

Bae¹,

Lee

et al. 2011

Gene

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“…정지기에 도달한 0.4 g의 배양세포를 100 mL의 현탁배양배지에 3~4주 간격으로 계대배양 하였다 (Lee et al 2005). 세포는 22±1°C의 약한 광조건 (20 µmol m -2 s -1 ) 에서 120 rpm으로 현탁배양 하였다.…”

Section: 식물재료 및 배양unclassified

“…배양세포에서 분리한 mRNA를 사용하여 제작한 cDNA library 유래의 EST (expressed sequence tag) 클론 분석 (Lee et al 2005 PCR 증폭산물의 정량을 위한 내부표준으로는 actin 유전자 를 사용하였다 (Kim et al 2011) Phylogenetic tree based on the PagDRM1 amino acid sequence and DRM sequences from Arabidopsis thaliana (At), Oryza sativa (Os), and Populus trichocarpa (Pt).The DRM family proteins used in this analysis include AtDRM1 (NP_001154378), AtDRM2 (NP_850220), AtDRM3 (NP_175809), AtDRM4 (NP_849820) and AtDRM5 (NP_199243) from Arabidopsis thaliana, OsDRM1 (ABA95234), OsDRM2 (ABA95871), OsDRM4 (NP_001061955), OsDRM5 (NP_001063265), OsDRM7 (AAL78369) and OsDRM8 (EEC83671) from Oryza sativa, and PtDRM1 (XP_002304241), PtDRM2 (XP_002305123), PtDRM3 (XP_002319507) and PtDRM4 (XP_002330171) from Populus trichocarpa.…”

Section: 유전자 분리 및 아미노산 서열 분석mentioning

confidence: 99%

Isolation and Expression of Dormancy-associated protein 1 (DRM1) in Poplar (Populus alba × P. glandulosa)

et al. 2017

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Dormancy-associated protein (DRM) is involved in the dormancy physiology of plants and is conserved in almost all plant species. Recent studies found that DRM genes are involved in the abiotic stress response, and characterization studies of these genes have been conducted in several plants. However, few studies have focused on DRM genes in woody plants. Therefore, in this study, cDNA coding for DRM (PagDRM1) was isolated from poplar (Populus alba × P. glandulosa), and its structure and expression characteristics were investigated. PagDRM1 encodes a putative protein composed of 123 amino acids, and the protein contains two conserved domains (Domain I and Domain II). PagDRM1 is present as one or two copies in the poplar genome. Its expression level was highest in the stem, followed by mature leaves, roots, and flowers. During the growth of cultured cells in suspension, PagDRM1 was highly expressed from the late-exponential phase to the stationary phase. In addition, PagDRM1 expression increased in response to drought, salt stress, and treatment with plant hormones (e.g., abscisic acid and gibberellic acid). Therefore, we suggested that PagDRM1 not only plays an important role in the induction of dormancy, but also contributes to stress tolerance in plants.

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“…현사시나무(Populus alba × P. glandulosa)의 배양세포에서 분리한 mRNA를 기반으로 제작된 cDNA library 유래의 EST (expressed sequence tag) 클론 분석 (Lee et al 2005 (Pfaffl, 2001).…”

Section: 유전자 분리 및 염기서열 분석unclassified

Characterization of Gibberellic Acid-Stimulated Arabidopsis (GASA) gene to drought stress response in Poplar (Populus alba × P. glandulosa)

et al. 2017

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Gibberellic Acid-Stimulated Arabidopsis (GASA) genes are involved in plant hormone signaling, cell division and elongation, as well as in responses to stress conditions in plants. In this study, we isolated a GASA gene from hybrid poplar (Populus alba × P. glandulosa) and analyzed its physiological phenotype and molecular functions in poplar.PagGASA cDNA encodes a putative protein composed of 95 amino acids containing an N-terminal signal peptide and a conservative cysteine-rich C-terminal domain. Southern blot analysis revealed that one or two copies of the PagGASA are present in the poplar genome. The PagGASA transcripts were highly detected in flowers and roots. Moreover, the expression of PagGASA was induced by growth hormone (gibberellic acid) and stress hormones (abscisic acid, jasmonic acid, and salicylic acid). By using transgenic analysis, we showed that the upregulation of PagGASA in poplar provides high tolerance to drought stress. Therefore, our results suggest that PagGASA plays an important role in drought stress tolerance via stress-related plant hormone signaling in poplar.

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Generation and analysis of expressed sequence tags from poplar (Populus alba×P. tremula var. glandulosa) suspension cells

Cited by 19 publications

References 28 publications

Drought, salt and wounding stress induce the expression of the plasma membrane intrinsic protein 1 gene in poplar (Populus alba×P. tremula var. glandulosa)

Drought, salt and wounding stress induce the expression of the plasma membrane intrinsic protein 1 gene in poplar (Populus alba×P. tremula var. glandulosa)

Isolation and Expression of Dormancy-associated protein 1 (DRM1) in Poplar (Populus alba × P. glandulosa)

Characterization of Gibberellic Acid-Stimulated Arabidopsis (GASA) gene to drought stress response in Poplar (Populus alba × P. glandulosa)

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