Characterization of Gibberellic Acid-Stimulated Arabidopsis (GASA) gene to drought stress response in Poplar (Populus alba × P. glandulosa)

Abstract

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Gibberellic Acid-Stimulated Arabidopsis (GASA) genes are involved in plant hormone signaling, cell division and elongation, as well as in responses to stress conditions in plants. In this study, we isolated a GASA gene from hybrid poplar (Populus alba × P. glandulosa) and analyzed its physiological phenotype and molecular functions in poplar.PagGASA cDNA encodes a putative protein composed of 95 amino acids containing an N-terminal signal peptide and a conservative cysteine-rich C-terminal domain. Southern blot analysis revealed that one or two copies of the PagGASA are present in the poplar genome. The PagGASA transcripts were highly detected in flowers and roots. Moreover, the expression of PagGASA was induced by growth hormone (gibberellic acid) and stress hormones (abscisic acid, jasmonic acid, and salicylic acid). By using transgenic analysis, we showed that the upregulation of PagGASA in poplar provides high tolerance to drought stress. Therefore, our results suggest that PagGASA plays an important role in drought stress tolerance via stress-related plant hormone signaling in poplar.

Genome-Wide Identification and Characterization of GASA Gene Family in Nicotiana tabacum

“…GASA influence various biological processes and signal transduction pathways, and then playing critical roles in plant growth and development (Choi et al, 2017). Due to complexities in functional mechanisms, different members of the GASA gene family have identical or diverse functions during the vegetative and reproductive stages.…”

Drought Stress-Mediated Transcriptome Profile Reveals NCED as a Key Player Modulating Drought Tolerance in Populus davidiana

“…In our laboratory, we developed a shoot regeneration technology from the leaf and shoot derived calli of the hybrid poplar (Populus alba × P. glandulosa) (Choi et al, 2020) for the successful generation of transgenic plants using Agrobacterium-mediated transformation (Chung et al, 1989;Choi et al, 2005;Song et al, 2019). Using hybrid poplar, transgenic plants have been successfully generated with several different genes involved in a variety of physiological responses (Zhang et al, 2011;Shim et al, 2013;Choi et al, 2017;Yoon et al, 2018;Kim et al, 2020). Since the hybrid poplar used for the transformation in this study is an artificial cross between P. alba and P. glandulosa, we found that the NCED3 DNA sequences of these two varieties were significantly similar to that of P. davidiana.…”