Genome-Wide Identification and Characterization of GASA Gene Family in Nicotiana tabacum

Li, Zhaowu; Gao, Junping; Wang, Genhong; Wang, Shuaibin; Chen, Kai; Pu, Wenxuan; Yaofu, Wang; Xia, Qingyou; Fan, Xing

doi:10.3389/fgene.2021.768942

Cited by 17 publications

(27 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Exon–intron structures were generated based on annotated C. rosea genome sequencing information using the Gene Structure Display Server program ( Figure 4 C). Even encoding relatively small proteins (70–233 aa), all CrGASA genes contain introns and some of them seem to be large ( CrGASA 22 and CrGASA 23 ), which is similar to that in other plant species [ 33 , 40 ]. Interestingly, in the highly homologous gene pairs CrGASA18 and CrGASA23 , CrGASA3 and CrGASA11 , and CrGASA2 and CrGASA16 , the gene structures were not very similar, and they had different numbers of exons.…”

Section: Resultsmentioning

confidence: 57%

“…Except for CrGASA12, the others ended with “KCP” at the C-terminus ( Figure 3 ). After knocking out the genome sequencing factors, the GASA domains in the CrGASA family seemed to be more variable than in other plants [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

“…The CrGASAs were divided into three groups (G1, G2, and G3) based on their phylogenetic analysis with other species, including Arabidopsis, rice, and soybean ( Figure 2 ). According to this phylogenetic tree, most CrGASAs showed closer homology with soybean GASAs, and, as a diploid dicotyledon species, C. rosea holds more CrGASA members than that in the diploid monocotyledons—for example, 14 or 10 GASA members were found in maize and rice [ 28 , 32 ], while almost 20 GASA members were identified in apple (26), citrus (18), tobacco (18), and poplars (19 or 21), which probably indicates a slight GASA gene expansion in dicotyledon species [ 35 , 36 , 37 , 39 , 40 ]. Because of the relatively short protein lengths and the highly conserved GASA domains of all CrGASAs ( Figure 3 ), the classification of protein groups mostly depends on the variable N-terminus ( Figure 4 ), which also determines the subcellular localization of CrGASAs.…”

Section: Discussionmentioning

confidence: 99%

“…The Citrus clementina canker pathogen Xanthomonas citri is the primary cause of citrus loss; some GASA s in the 18-member CcGASA family are speculated to respond to X. citri infection [ 39 ]. Tobacco ( Nicotiana tabacum ) has 18 GASA genes, and some NtGASA s display unique or distinct expression patterns in different tissues, suggesting their potential roles in tobacco plant growth and development [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Genome-Wide Identification and Functional Analysis of the GASA Gene Family Responding to Multiple Stressors in Canavalia rosea

Zhang

Wang

Jian

2022

Genes

View full text Add to dashboard Cite

In plants, the Gibberellic Acid-Stimulated Arabidopsis (GASA) gene family is unique and responds to ubiquitous stress and hormones, playing important regulatory roles in the growth and development of plants, as well as in the resistance mechanisms to biotic and abiotic stress. In this study, a total of 23 CrGASAs were characterized in C. rosea using a genome-wide approach, and their phylogenetic relationships, gene structures, conserved motifs, chromosomal locations, gene duplications, and promoter regions were systematically analyzed. Expression profile analysis derived from transcriptome data showed that CrGASAs are expressed at higher levels in the flowers or fruit than in the leaves, vines, and roots. The expression of CrGASAs also showed habitat- and environmental-stress-regulated patterns in C. rosea analyzed by transcriptome and quantitative reverse transcription PCR (qRT-PCR). The heterologous induced expression of some CrGASAs in yeast enhanced the tolerance to H2O2, and some CrGASAs showed elevated heat tolerance and heavy metal (HM) Cd/Cu tolerance. These findings will provide an important foundation to elucidate the biological functions of CrGASA genes, especially their role in the ecological adaptation of specific plant species to tropical islands and reefs in C. rosea.

show abstract

Section: Resultsmentioning

confidence: 57%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genome-Wide Identification and Functional Analysis of the GASA Gene Family Responding to Multiple Stressors in Canavalia rosea

Zhang

Wang

Jian

2022

Genes

View full text Add to dashboard Cite

show abstract

“…The N. tabacum L. K326 plants were cultured in a greenhouse with conditions described in previous report ( Li Z. et al, 2021 ). For expression profiles of the NtLHT genes in different tissues, total RNA was isolated from the roots, axillary buds, stems, flowers, and leaves of tobacco at the flowering stage.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive analysis of the LHT gene family in tobacco and functional characterization of NtLHT22 involvement in amino acids homeostasis

Gao

Wang

et al. 2022

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Amino acids are vital nitrogen (N) sources for plant growth, development, and yield. The uptake and translocation of amino acids are mediated by amino acid transporters (AATs). The AATs family including lysine-histidine transporters (LHTs), amino acid permeases (AAPs), and proline transporters (ProTs) subfamilies have been identified in various plants. However, little is known about these genes in tobacco. In this study, we identified 23 LHT genes, the important members of AATs, in the tobacco genome. The gene structure, phylogenetic tree, transmembrane helices, chromosomal distribution, cis-regulatory elements, and expression profiles of NtLHT genes were systematically analyzed. Phylogenetic analysis divided the 23 NtLHT genes into two conserved subgroups. Expression profiles confirmed that the NtLHT genes were differentially expressed in various tissues, indicating their potential roles in tobacco growth and development. Cis-elements analysis of promoters and expression patterns after stress treatments suggested that NtLHT genes probable participate in abiotic stress responses of tobacco. In addition, Knock out and overexpression of NtLHT22 changed the amino acids homeostasis in the transgenic plants, the contents of amino acids were significantly decreased in NtLHT22 overexpression plants than wild-type. The results from this study provide important information for further studies on the molecular functions of the NtLHT genes.

show abstract

Antimicrobial peptides in Dendrobium officinale: Genomic parameters, peptide structures, and gene expression patterns

et al. 2023

View full text Add to dashboard Cite

A weak codon usage bias was found in Dendrobium catenatum (D. officiale) antimicrobial peptides (AMPs), after the analysis of relative synonymous codon usage, GC contents, and the effective number of codons. The codon usage preference was mainly influenced by natural selection pressure. The self‐optimized prediction method and SWISS‐MODEL were applied for peptide structural and domain analyses, and some typical antimicrobial domains were found in D. officinale AMP amino sequences, such as knot1 domain, gibberellins‐stimulated domain, cupin_1 domain, defensin_like domain, and SLR1‐BP (S locus‐related glycoprotein 1 binding pollen coat protein) domain. To investigate the AMPs gene expression pattern, abiotic stresses, such as salt stress, drought stress, salicylic acid (SA), and methyl jasmonate (JA), were applied and the gene expression levels were detected by the real‐time fluorescent quantitative polymerase chain reaction. Results showed that, even though the basic AMPs gene expressions were low, some AMPs can still be induced by salt dress, while the drought dress did not show the same impact. The SA and JA signaling pathways might be involved in most of the AMPs expressions. The natural selection of the D. officinale AMPs and thus forming diverse types of AMPs enhanced the plant's innate immunity and disease resistance capability, which would lead to a better understanding of the molecular mechanism for D. officinale adapting to the environment. The finding that salt stress, SA, and JA signaling pathways can induce AMP expression lays a foundation for the further development and functional verification of D. officinale AMPs.

show abstract

Genome-Wide Identification and Characterization of GASA Gene Family in Nicotiana tabacum

Cited by 17 publications

References 54 publications

Genome-Wide Identification and Functional Analysis of the GASA Gene Family Responding to Multiple Stressors in Canavalia rosea

Genome-Wide Identification and Functional Analysis of the GASA Gene Family Responding to Multiple Stressors in Canavalia rosea

Comprehensive analysis of the LHT gene family in tobacco and functional characterization of NtLHT22 involvement in amino acids homeostasis

Antimicrobial peptides in Dendrobium officinale: Genomic parameters, peptide structures, and gene expression patterns

Contact Info

Product

Resources

About