Generalized polymer effective charge measurement by capillary isotachophoresis

Chamieh, Joseph; Koval, Dušan; Besson, Adeline; Kašička, Václav; Cottet, Hervé

doi:10.1016/j.chroma.2014.10.025

Cited by 12 publications

(13 citation statements)

References 41 publications

(60 reference statements)

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“…As follows from the data in Table , the experimentally determined effective charges and ionic mobilites of AMPs were obtained with a good precision, with RSDs mostly less than 5% for the effective charges and always less than 2% for the ionic mobilities. These values are in a good agreement with the general estimation of 5–10% uncertainty of the effective charges determined by ITP for solutes with mobilities in the 20–50 × 10 −9 m 2 V −1 s −1 range and with a common 2% RSD of CZE determined mobilities.…”

Section: Resultssupporting

confidence: 86%

“…In the previous reports , a procedure for calculation of the effective charge number, z i,eff , of the i ‐th charged species was developed and Eq. was derived:

\frac{z_{normali, eff}}{z_{ref}} = \frac{Δ t_{normali} c_{ref} V_{ref}}{Δ t_{ref} c_{normali} V_{normali}} \frac{μ_{i, z} (μ_{ref, z} + μ_{c, z})}{μ_{ref, z} (μ_{i, z} + μ_{c, z})}

where subscripts i , ref , and c refer to the species of interest, reference compounds, and counterion in the corresponding ITP electrolyte system, respectively, Δt is the ITP zone length in time units, V is the injected sample volume, c is the molar concentration, and μ is the actual ionic mobility, i.e.…”

Section: Theorymentioning

confidence: 99%

“…In the previous reports [39,40], a procedure for calculation of the effective charge number, z i,eff , of the i-th charged species was developed and Eq. (3) was derived:…”

Section: Determination Of Effective Chargementioning

confidence: 99%

“…Different methods have been used for effective charge determination including electric conductivity [27] and osmotic pressure measurements [28], light scattering techniques [29], and electrophoretic methods [21,22,24,26,[30][31][32][33][34][35][36][37][38]. Capillary isotachophoresis (CITP) was recently introduced as a suitable method for determination of effective charge of nanoparticles [32] and polyelectrolytes [39,40]. This method is based on the linear dependence of the ITP zone length on the solute charge and concentration.…”

Section: Introductionmentioning

confidence: 99%

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Determination of effective charges and ionic mobilities of polycationic antimicrobial peptides by capillary isotachophoresis and capillary zone electrophoresis

et al. 2017

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Capillary ITP (CITP) and CZE were applied to the determination of effective charges and ionic mobilities of polycationic antimicrobial peptides (AMPs). Twelve AMPs (deca- to hexadecapeptides) containing three to seven basic amino acid residues (His, Lys, Arg) at variable positions of peptide chain were investigated. Effective charges of the AMPs were determined from the lengths of their ITP zones, ionic mobilities, and molar concentrations, and from the same parameters of the reference compounds. Lengths of the ITP zones of AMPs and reference compounds were obtained from their CITP analyses in cationic mode using leading electrolyte (LE) composed of 10 mM NH OH, 40 mM AcOH (acetic acid), pH 4.1, and terminating electrolyte (TE) containing 40 mM AcOH, pH 3.2. Ionic mobilities of AMPs and singly charged reference compounds (ammediol or arginine) were determined by their CZE analyses in the BGE of the same composition as the LE. The effective charges numbers of AMPs were found to be in the range 1.65-5.04, i.e. significantly reduced as compared to the theoretical charge numbers (2.86-6.99) calculated from the acidity constants of the analyzed AMPs. This reduction of effective charge due to tightly bound acetate counterions (counterion condensation) was in the range 17-47% depending on the number and type of the basic amino acid residues in the AMPs molecules. Ionic mobilities of AMPs achieved values (26.5-38.6) × 10 m V s and in most cases were in a good agreement with the ratio of their effective charges and relative molecular masses.

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Section: Resultssupporting

confidence: 86%

“…In the previous reports , a procedure for calculation of the effective charge number, z i,eff , of the i ‐th charged species was developed and Eq. was derived:

\frac{z_{normali, eff}}{z_{ref}} = \frac{Δ t_{normali} c_{ref} V_{ref}}{Δ t_{ref} c_{normali} V_{normali}} \frac{μ_{i, z} (μ_{ref, z} + μ_{c, z})}{μ_{ref, z} (μ_{i, z} + μ_{c, z})}

Section: Theorymentioning

confidence: 99%

“…In the previous reports [39,40], a procedure for calculation of the effective charge number, z i,eff , of the i-th charged species was developed and Eq. (3) was derived:…”

Section: Determination Of Effective Chargementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of effective charges and ionic mobilities of polycationic antimicrobial peptides by capillary isotachophoresis and capillary zone electrophoresis

et al. 2017

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“…However, ITP or t‐ITP is frequently applied as preconcentration and/or preseparation steps prior to CE analysis of cationic or anionic analytes including peptides present at low concentrations and/or in complex mixtures. In addition, CITP can be applied also for determination of effective charge of native or synthetic polypeptides and other (bio)polymers .…”

Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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Capillary Electromigration Techniques: Capillary Electrophoresis

Kašička

2015

Analytical Separation Science

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This chapter provides a brief introduction to capillary electrophoresis ( CE ) – a modern high‐performance electromigration separation method. First, basic concepts of electrophoresis in general, such as ionic, effective and limiting electrophoretic mobilities, their concentration, and pH dependencies are presented, and the concomitant electrokinetic phenomenon – electroosmotic flow – is introduced. Second, the separation principle and the basic experimental setup of CE are shown and the advantages and limitations of CE as compared to other separation methods, especially liquid chromatography and slab gel electrophoresis, are reported. Further, the dispersion effects in CE and the ways of minimization of their detrimental effects on the separation efficiency of CE are described. Finally, the high application potential of CE for highly sensitive qualitative and quantitative analysis, microscale isolation, and physicochemical and biochemical characterization of a large spectrum of all types of ionogenic compounds and (bio)particles is emphasized. For more detailed studies of CE and other capillary electromigration methods, many literature references are given.

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Generalized polymer effective charge measurement by capillary isotachophoresis

Cited by 12 publications

References 41 publications

Determination of effective charges and ionic mobilities of polycationic antimicrobial peptides by capillary isotachophoresis and capillary zone electrophoresis

Determination of effective charges and ionic mobilities of polycationic antimicrobial peptides by capillary isotachophoresis and capillary zone electrophoresis

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Capillary Electromigration Techniques: Capillary Electrophoresis

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