Generalist predators consume spider mites despite the presence of alternative prey

Krey, Karol L.; Blubaugh, Carmen K.; Chapman, Eric G.; Lynch, Christine A.; Snyder, Gretchen B.; Jensen, Andrew S.; Fu, Zhen F.; Prischmann‐Voldseth, Deirdre A.; Harwood, James D.; Snyder, William E.

doi:10.1016/j.biocontrol.2017.10.007

Cited by 22 publications

(26 citation statements)

References 61 publications

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“…Successful biological control of agricultural pests requires a thorough knowledge of the trophic relationships, especially between the second and the third trophic level (i.e., phytophagous pests and their natural enemies). However, the study and understanding of these interactions can be highly challenging, especially when generalist predators that frequently exploit multiple prey species may interact either with the target pest, alternative prey or food sources . In this case, the only conclusive evidence of predation is direct observation of prey consumption by a predator and identification of the prey remains within the predator's gut or faeces.…”

Section: Introductionmentioning

confidence: 99%

Tracking mite trophic interactions by multiplex PCR

Gómez‐Martínez

Piña

Aguilar‐Fenollosa

et al. 2019

Pest Management Science

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BACKGROUND: A thorough knowledge of trophic webs in agroecosystems is essential to achieve successful biological pest control. Phytoseiid mites are the most efficient natural enemies of tetranychid mites, which include several important pests worldwide. Nevertheless, phytoseiids may feed on other food sources including other microarthropods, plants and even other phytoseiids (intraguild predation), which can interfere with biological control services. Molecular gut content analysis is a valuable tool for characterizing trophic interactions, mainly when working on microarthropods such as mites. We have designed new primers for Phytoseiidae, Tetranychidae and Thysanoptera identification and they have been multiplexed in a polymerase chain reaction (PCR) together with universal plant primers. Additionally, we have estimated prey DNA detectability success over time (DS 50 ) considering the most probable events in Spanish citrus orchards: the phytoseiid Euseius stipulatus as a predator, the phytoseiid Phytoseiulus persimilis as intraguild prey, and the thrips Frankliniella occidentalis and Anaphothrips obscurus as alternative prey to Tetranychus urticae. gut of the phytoseiid predator. DS 50 for E. stipulatus as the predator were 1.3, 2.3 and 18.7 h post feeding for F. occidentalis, A. obscurus and P. persimilis as prey, respectively. CONCLUSION: Tracking of the trophic relationships within the citrus acarofauna, and the unveiling of the role of alternative food sources will pave the way for enhancing T. urticae biological control. This multiplex PCR approach could be applicable for these purposes in similar agroecosystems. RESULTS: The designed multiplex PCR allows the identification of phytoseiids (both predator and intraguild prey) and detects alternative food sources mentioned above in the DNA extractionDNA of Phytoseiidae, Tetranychidae, Thysanoptera and other potential food sources for Phytoseiidae mites in citrus orchards and those provided in the laboratory rearings was extracted following different protocols depending on the organism and the objective of the study.Acari and Hemiptera from the UJI-IVIA DNA collection and new Acari DNA used for feeding trials (Tables 1 and 3), were previously Pest Manag Sci 2020; 76: 597-608

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Section: Introductionmentioning

confidence: 99%

Tracking mite trophic interactions by multiplex PCR

Gómez‐Martínez

Piña

Aguilar‐Fenollosa

et al. 2019

Pest Management Science

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“…Multi2 PCR protocols were as follows: 95°C for 15 min, and 35 cycles of 94°C for 30 s, 63°C for 1 min 30 s, 72°C for 30 s, and a final extension of 72°C for 10 min. A singleplex PCR reaction was used to screen predators for an additional incidental pest, the twospotted spider mite (Tetranychus urticae) (Krey et al, 2017). Aphids and spider mites are common pests of several cropping systems but do not consistently reach economically significant levels on cotton in the region (Rosenheim et al, 1997), and are therefore treated here as incidental pests.…”

Section: Molecular Gut-content Analysismentioning

confidence: 99%

Beyond soil health: the trophic effects of cover crops shape predator communities

Bowers¹,

Toews

Schmidt

2020

Preprint

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Maintaining habitat throughout the season in annual cropping systems provides resource stability for arthropod communities. Stabilizing resource availability should lead to diverse predatory communities and their associated ecosystem services such as biological control. There is a need for studies to test change in predator communities due to habitat provisioning and estimate associated food web responses. Here we quantified predator community structure and used molecular gut-content analysis to reconstruct predator food webs in response to winter cover crops (i.e. cereal and legume based) in a cotton agroecosystem. Predators were collected from experimental field plots during each major crop development stage in 2017 and 2018, and PCR was used to estimate predator roles and responses to cover crop treatments. Cotton planted into a rye cover crop residue promoted unique predator communities in the early and mid-season as compared to no-cover fields. Correspondingly, we observed dissimilar prey consumption among cover crop treatments. While predators consumed incidental pests at high frequencies (e.g. aphids), predation on key pests by natural enemies in this system was lacking. The use of winter cover crops and reduced tillage practices increased the consumption of alternative prey by natural enemies on seedling cotton, encouraging high predator diversity that aligns temporally with potential early season pest outbreaks. Therefore, cover crops should be further integrated into integrated pest management strategies.

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“…Damsel bugs are commonly found on many plants, including field crops such as alfalfa, cotton, and soybean, as well as in orchards (Braman and Yeargan 1990;Dinkins et al 1970;Miliczky and Horton 2005). Krey et al (2017) found high populations of Nabis in commercial potato fields in Washington. Nabis capsiformis have recently been found in organic strawberry fields of central Florida (K. Krey unpublished).…”

Section: Host Plantsmentioning

confidence: 99%

Pale Damsel Bug Nabis capsiformis Germar (Insecta: Hemiptera: Nabidae)

Krey¹,

Renkema²

2018

EDIS

Self Cite

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The pale damsel bug, Nabis capsiformis (Germar), one of the most widespread Nabis species, is a generalist predator that can be found in open areas, including coastal habitats (Lattin 1989). It is commonly reported throughout the southeastern United States, in a variety of ecosystems, it is particularly common in field and row crop agroecosystems. All nympal and adult life stages of Nabis capsiformis are excellent predators and can typically eat one lepidopteran egg or aphid per day when small and as many as two dozen eggs or other prey as later instars and adults. They can survive for up to two weeks without food and become cannibalistic if other prey are unavailable. They use their thickened raptorial front legs that are lined with spines to catch and hold prey, then suck out the body contents with their piercing mouthparts. Damsel bugs, including the pale damsel bug, are so named because of the way they hold their front legs up, as though they were lifting a skirt hem to curtsey. At first glance, they appear like a cross between a mantis and an assassin bug. Includes: Introduction - Distribution - Description - Host Plants - Life Cycle - Importance - Selected References.https://edis.ifas.ufl.edu/in1210 Also published at: https://entomology.ifas.ufl.edu/creatures/VEG/pale_damsel_bug.html

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Generalist predators consume spider mites despite the presence of alternative prey

Cited by 22 publications

References 61 publications

Tracking mite trophic interactions by multiplex PCR

Tracking mite trophic interactions by multiplex PCR

Beyond soil health: the trophic effects of cover crops shape predator communities

Pale Damsel Bug Nabis capsiformis Germar (Insecta: Hemiptera: Nabidae)

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