General Media and Vessels Suitable for Woody Plant Culture

Factors controlling micropropagation of Myrica esculenta buch. Ham. ex D. Don: a high value wild edible of Kumaun Himalaya

“…Reducing the salt concentration in MS resulted in poor performance with regard to percent establishment, shoot number and shoot length as compared to WPM. Some species give similar response in all media while others show preference for a specific medium for explant establishment and growth (McCown and Sellmer, 1987). In the present study, WPM gave better results than B 5 , MS and 1/2 MS.…”

“…Addition of 10 µM kinetin with 0.1 µM NAA was found most suitable for explant establishment. The effectiveness of cytokinin in promoting axillary bud development in vitro in forest trees is well documented (Zaerr and Mapes, 1982;McCown and Sellmer, 1987). Addition of low level of auxin along with cytokinin has been known to increase percent establishment as well as shoot number in forest trees (Rathore et al, 1991).…”

“…Reports suggest that in B 5, explants do not respond well due to the presence of high ammonium and nitrate content (Constabel, 1984), which inhibit percent establishment and shoot multiplication. In the present study, the best results in WPM, were probably due to lower concentration of salts and sucrose compared to B 5 and MS. Low concentration of sucrose is reported to reduce browning in Pseudotsuga menziesii cultures (Evers, 1984) and weaker salt formulations promote axillary bud development in forest trees (McCown and Sellmer, 1987).…”

Physiological and developmental problems encountered by in vitro cultured plants

“…Shoot-tip necrosis is a phenomenon whereby the apical shoot becomes brown and later dies (McCown and Sellmer 1987). The problem of non-pathogenic dieback or shoot-tip necrosis of in vitro cultures has been associated with various culture conditions.…”

Effect of modifying concentrations of calcium and magnesium on in vitro development of banana CV. Prata-Anã (Genomic group AAB)

“…Ca is transported through passive processes that are influenced by the respiration rate (MCCOWN;SELLMER, 1987). Therefore, the necrotic symptoms observed in the terminal shoots mostly occurred as a result of low respiratory activity in the explants cultivated in vitro.…”