“…Reports suggest that in B 5, explants do not respond well due to the presence of high ammonium and nitrate content (Constabel, 1984), which inhibit percent establishment and shoot multiplication. In the present study, the best results in WPM, were probably due to lower concentration of salts and sucrose compared to B 5 and MS. Low concentration of sucrose is reported to reduce browning in Pseudotsuga menziesii cultures (Evers, 1984) and weaker salt formulations promote axillary bud development in forest trees (McCown and Sellmer, 1987).…”