General, high-affinity approach for the synthesis of fluorophore appended protein nanoparticle assemblies

Abstract

Metallothionein fusion proteins allow for site-specific, orthogonal functionalization of proteins to a variety of nanoparticles.

Dissociation coefficients of protein adsorption to nanoparticles as quantitative metrics for description of the protein corona: A comparison of experimental techniques and methodological relevance

“…in the case fluorescence of the proteins and the NPs is quenched the NPs and the proteins are the quenchers, respectively) nonequilibrium quenching this can be described by the Stern-Volmer equation [61,62]. In this approach the quenching efficiency depends on the degree of shielding of the fluorophores by the quenchers, and thus reveals the relative accessibility of quenchers to the fluorophores, which is done by a diffusion model [60,63,64]. The Stern-Volmer constant K SV can be considered to be reciprocal to the dissociation constant K D ≈ 1/K SV , though actually K D refers to equilibrium and 1/K SV to nonequilibrium conditions.…”

Wide Dynamic Range Sensing with Single Quantum Dot Biosensors

“…These ferroceneÀMBPÀ CdSe@ZnS nanoparticle systems were assembled as before, 7,17,21 where mercaptohexadecanoate-capped CdSe@ZnS nanoparticles (560 nm emitting) were incubated with 1 equiv of 1-attached MBP-His 6 for 90 min. Binding was followed as a decrease in emission at 560 nm (N282C, 30%; F92C, 47%; N95C, 10%) and…”

“…22 Surface cysteine MBPs (N282C, F92C, and N95C) were modified with 1 using similar conditions as modification with (tetraammine)(5-maleimido-1,10-phenanthroline)-ruthenium(II) bis(hexafluorophosphate) previously. 7 The 1-modified MBPs with a six histidine tag on the carboxy termini were assembled onto MHDA-capped, ZnS-coated, CdSe nanoparticles (CdSe@ZnS) as reported before 21,40 and confirmed to have one 1-appended MBP bound per CdSe@ZnS nanoparticle.…”

Nanoparticles and Sensors

“…Direct Attachment to QDs: The attachment through thiol group or metal-affinity-driven coordination is one of the most direct methods to conjugate QDs with biomolecules. Recent synthetic progress has allowed the conjugation of QDs to a wide variety of biomolecules such as DNA, proteins, antibodies, and peptides [39][40][41][42][43].…”