General features of gastric carcinomas and comparison of HSP70 and NK cell immunoreactivity with prognostic factors

Canöz, Özlem; Belenli, Olcay; Patıroğlu, Tahir E.

doi:10.1007/bf03036742

Cited by 28 publications

(19 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In several studies of human gastric carcinomas, correlations between Hsp70 expression and malignancy parameters of poor prognosis have been reported, 3 but there are no published studies reporting correlation in veterinary medicine.…”

Section: Discussionmentioning

confidence: 99%

“…The immunoexpression indexes were calculated as the average of percentage of positive cells in 5 representative fields for each tumor. 3,10,18,19,23,26,35,44 For each tissue section, staining was scored as absent (grade 0), mild (grade 1: <10% positive cells), moderate (grade 2: 10-50% positive cells), and high (grade 3: >50% positive cells). Grades 0 and 1 were considered to be negative.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Canine Gastric Carcinoma

et al. 2010

View full text Add to dashboard Cite

Immunohistochemical staining for cell cycle proteins and heat shock proteins was performed on 17 canine gastric carcinomas. The immunoexpression of p53, p21, p16, Hsp27, and Hsp70 was investigated. A study was conducted to determine the histological type and parameters related to tumor malignancy. Possible associations and trends were assessed between the immunoexpression of each protein and tumor type as well as specific parameters of malignancy. High intratumor frequency of cellular p53 immunostaining was observed (61.96% average), but lower frequencies of p21 and p16 expression were present (34.65% and 10.41%, respectively). The p53 overexpression was associated with tumor infiltration (P ¼ .0258). Expression of p21 was lower in undifferentiated carcinomas, and the loss of expression was associated with histopathological parameters characteristic of a poor prognosis such as lymphatic vessel invasion (P ¼ .0258). The lack of p16 immunoreactivity was related to histopathological characteristics of malignancy such as the presence of evident and multiple nucleoli (P ¼ .0475). In contrast, deep tumor infiltration was observed in those carcinomas with a high p16 index (P ¼ .0475). Hsp70 appeared to be overexpressed in all gastric neoplasms included in this study. This is in contrast to Hsp27, because a group of tumors showed complete lack of Hsp27 immunoexpression, whereas the others displayed extensive Hsp27 immunostaining. The differences in Hsp27 did not correlate with any of the histopathological parameters, but Hsp27 immunoexpression was higher in the undifferentiated carcinoma. No significant differences in the expression of the proteins were found in canine gastric carcinomas according to their histological type. These findings may be useful for establishing a prognosis for canine gastric carcinoma.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Canine Gastric Carcinoma

et al. 2010

View full text Add to dashboard Cite

show abstract

“…In tumor cell lines, the increase in HSP70 expression has been shown to stimulate cell proliferation [36], while specific inhibition of HSP70 expression suppressed cell proliferation and increased apoptosis [37]. Our data showed that live H. pylori decreased HSP70 expression in gastric epithelial cells.…”

Section: Discussionmentioning

confidence: 63%

Effects ofHelicobacter pyloriand Heat Shock Protein 70 on the Proliferation of Human Gastric Epithelial Cells

Tao

Zou

Huang

2014

Gastroenterology Research and Practice

View full text Add to dashboard Cite

Infection of Helicobacter pylori (H. pylori) changed the proliferation of gastric epithelial cells and decreased the expression of heat shock protein 70 (HSP70). However, the effects of H. pylori on the proliferation of gastric epithelial cells and the roles of HSP70 during the progress need further investigation. Objective. To investigate the effects of Helicobacter pylori (H. pylori) and heat shock protein 70 (HSP70) on the proliferation of human gastric epithelial cells. Methods. H. pylori and a human gastric epithelial cell line (AGS) were cocultured. The proliferation of AGS cells was quantitated by an MTT assay, and the expression of HSP70 in AGS cells was detected by Western blotting. HSP70 expression in AGS cells was silenced by small interfering RNA (siRNA) to investigate the role of HSP70. The siRNA-treated AGS cells were cocultured with H. pylori and cell proliferation was measured by an MTT assay. Results. The proliferation of AGS cells was accelerated by coculturing with H. pylori for 4 and 8 h, but was suppressed at 24 and 48 h. HSP70 expression was decreased in AGS cells infected by H. pylori for 48 h. The proliferation in HSP70-silenced AGS cells was inhibited after coculturing with H. pylori for 24 and 48 h compared with the control group. Conclusions. Coculture of H. pylori altered the proliferation of gastric epithelial cells and decreased HSP70 expression. HSP70 knockdown supplemented the inhibitory effect of H. pylori on proliferation of epithelial cells. These results indicate that the effects of H. pylori on the proliferation of gastric epithelial cells at least partially depend on the decreased expression of HSP70 induced by the bacterium.

show abstract

“…In addition, although HSP expression was recognized as a prognostic value in certain tumors, the results are contradictory [23][24][25]. The study of Gefeller et al [26] showed that in Wilms tumor, HSP70 protein was confined to the tumor from children who survived, whereas tumors from dead patients were negative or weakly positive for HSP70.…”

Section: Discussionmentioning

confidence: 97%