General Aspects of Secondary Metabolism

Döhren, Hans von; Gräfe, U.

doi:10.1002/9783527620890.ch1

Cited by 17 publications

(3 citation statements)

References 249 publications

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“…Streptomyces and many other soil-dwelling actinomycetes produce an impressive array of secondary metabolites, many of them having important biological activities (Crandall and Hamill, 1986;Von Döhren and Gräfe, 1997;Martín et al, 2000). Nevertheless, very little is known about the regulation of secondary metabolism in Streptomyces.…”

Section: Introductionmentioning

confidence: 99%

Cross‐talk between two global regulators in Streptomyces: PhoP and AfsR interact in the control of afsS, pstS and phoRP transcription

Santos-Beneit

Rodrı́guez-Garcı́a

Sola‐Landa

et al. 2009

Molecular Microbiology

111

112

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Section: Introductionmentioning

confidence: 99%

Cross‐talk between two global regulators in Streptomyces: PhoP and AfsR interact in the control of afsS, pstS and phoRP transcription

Santos-Beneit

Rodrı́guez-Garcı́a

Sola‐Landa

et al. 2009

Molecular Microbiology

111

112

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“…They exhibit a remarkable capacity for biochemical differentiation by producing a wide variety of secondary metabolites that permit the adaptation to stress conditions and give them a competitive advantage over other microorganisms (von Döhren and Gräfe 1997). They are quantitatively and qualitatively important in the rhizosphere, as they may protect plant roots against invasive root pathogenic bacteria and fungi (Crawford et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of isolate HL-12 against Clavibacter michiganensis subsp. michiganensis in the presence of cadmium

Yuan

Min

et al. 2009

Ecotoxicology

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An actinomycete, strain HL-12, that was isolated from a farmland on the Huajiachi campus of Zhejiang University was capable of inhibiting the growth of Clavibacter michiganensis subsp. michiganensis (Cmm) and was identified as a member of Streptomyces. Its antimicrobial activity against Cmm was measured using the agar plate sensitivity method in pure culture and evaluated by the inhibition ratio of Cmm in soil. The inhibitory activity of strain HL-12 against Cmm following exposure to low concentrations of Cd was greater than the inhibitory activity following exposure to high concentrations of Cd both in liquid culture and in soil. A stronger inhibition was also seen following a 24 h preculture in the presence of Cd in liquid culture. The growth of Cmm in soil was stimulated at low concentrations of Cd (<5.0 mg Cd kg(-1) dry soil) but inhibited when cultured in high concentrations of Cd (5.0 and 10.0 mg Cd kg(-1) dry soil). A higher inhibition ratio of strain HL-12 against Cmm, which was over 40% after soil incubation for 2 weeks, was observed following exposure to low concentrations of Cd (<5.0 mg Cd kg(-1) dry soil).

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“…After isolation of monocultures of both strains, we found that the anaerobe was the source of the antibiotic activity. As the reports on antibiotics produced by strictly anaerobic bacteria are few [1,2], we tried to isolate the antibiotic from this anaerobic bacterium. The attempt resulted in the discovery of naphthalecin.…”

Section: Introductionmentioning

confidence: 99%

Naphthalecin, a Novel Antibiotic Produced by the Anaerobic Bacterium, Sporotalea colonica sp. nov.

et al. 2008

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A novel antibiotic naphthalecin was purified and isolated from the cells of an anaerobic bacterium isolated from a soil sample. This antibiotic contained a naphthalene moiety, so named as naphthalecin, and showed antibacterial activity against gram positive species. The producing strain, an obligate anaerobe, was identified as a new species of the genus Sporotalea. Identification of the bacterium, cultivation, purification, structure determination, and antibacterial activity are shown.Keywords antibacterial antibiotic, strictly anaerobic bacteria, naphthalene, naphthalecin, Sporotalea colonica IntroductionDuring the screening of bacterial isolates from soil samples, an obligate anaerobic bacterium growing in a colony of an aerobic bacterium and a coculture of both strains with an antibacterially active mycelial form were found. After isolation of monocultures of both strains, we found that the anaerobe was the source of the antibiotic activity. As the reports on antibiotics produced by strictly anaerobic bacteria are few [1, 2], we tried to isolate the antibiotic from this anaerobic bacterium. The attempt resulted in the discovery of naphthalecin. Results Taxonomic StudyStrain SYB was a strictly anaerobic Gram-positive bacterium. Microscopic observation showed long, straight rods occurring singly or in pairs. As shown in Fig. 1, cells measured 2.0ϳ15.0 m m in length and 0.5ϳ0.8 m m in diameter and were motile with peritrichous flagella. In older cultures, cells formed refractile terminal endospores which were heat resistant (80°C, 10 minutes). Growth on BP agar was poor; a 14-day culture yielded small, circular, yellow colonies about 2ϳ3 mm in diameter. The temperature and pH ranges for growth were 15ϳ35°C (optimal 25ϳ30°C), and 6.0ϳ9.0 (optimal 7.0ϳ8.0), respectively.The 16S rRNA gene sequence of strain SYB determined in this study was almost complete, comprising 1,419 nt. The sequence showed that strain SYB was most closely affiliated with a strain of the genus Sporotalea [3,4]. A phylogenetic tree based on the neighbour-joining algorithm Naphthalecin, a Novel Antibiotic Produced by the Anaerobic Bacterium, Sporotalea colonica sp. nov.

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General Aspects of Secondary Metabolism

Cited by 17 publications

References 249 publications

Cross‐talk between two global regulators in Streptomyces: PhoP and AfsR interact in the control of afsS, pstS and phoRP transcription

Cross‐talk between two global regulators in Streptomyces: PhoP and AfsR interact in the control of afsS, pstS and phoRP transcription

Antimicrobial activity of isolate HL-12 against Clavibacter michiganensis subsp. michiganensis in the presence of cadmium

Naphthalecin, a Novel Antibiotic Produced by the Anaerobic Bacterium, Sporotalea colonica sp. nov.

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