Antimicrobial activity of isolate HL-12 against Clavibacter michiganensis subsp. michiganensis in the presence of cadmium

Abstract

An actinomycete, strain HL-12, that was isolated from a farmland on the Huajiachi campus of Zhejiang University was capable of inhibiting the growth of Clavibacter michiganensis subsp. michiganensis (Cmm) and was identified as a member of Streptomyces. Its antimicrobial activity against Cmm was measured using the agar plate sensitivity method in pure culture and evaluated by the inhibition ratio of Cmm in soil. The inhibitory activity of strain HL-12 against Cmm following exposure to low concentrations of Cd was greater than the inhibitory activity following exposure to high concentrations of Cd both in liquid culture and in soil. A stronger inhibition was also seen following a 24 h preculture in the presence of Cd in liquid culture. The growth of Cmm in soil was stimulated at low concentrations of Cd (<5.0 mg Cd kg(-1) dry soil) but inhibited when cultured in high concentrations of Cd (5.0 and 10.0 mg Cd kg(-1) dry soil). A higher inhibition ratio of strain HL-12 against Cmm, which was over 40% after soil incubation for 2 weeks, was observed following exposure to low concentrations of Cd (<5.0 mg Cd kg(-1) dry soil).

Production of DAPG and HCN by Pseudomonas sp. LBUM300 Contributes to the Biological Control of Bacterial Canker of Tomato

“…and other antagonistic microorganisms (4) including Streptomyces spp. (33), but no demonstration of the potential mechanisms involved, as it is reported in this study using isogenic mutant strains, was provided.…”

“…Umesha (25) also showed that tomato canker incidence could be reduced by P. fluorescens seed treatments. Streptomyces spp., lysosyme, and available commercial biocontrol products have also showed positive results (27,33). However, to the best of our knowledge, the impact of the production of secondary metabolites such as DAPG and HCN on bacterial canker disease symptom development had never been reported before.…”

“…Copper-based chemical treatments have traditionally been used in an attempt to control the disease (12,31). More recently, plant activators for resistance induction (22) and biological control (3,4,25,27,30,(32)(33)(34) have also been proposed as control strategies. However, none of these approaches have so far proven to be successful for reliably and consistently controlling bacterial canker of tomato.…”

Clavibacter michiganensis ssp. michiganensis: bacterial canker of tomato, molecular interactions and disease management

“…Other than pseudomonads, microorganisms with Cmm ‐inhibiting activity under glasshouse conditions include Streptomyces sp. strain HL‐12 (Yuan et al ., ), Bacillus subtilis , Trichoderma harzianum and Rhodosporidium diobovatum (Utkhede and Koch, ).…”

Antibiotic Resistance in Plant Pathogenic Bacteria: Recent Data and Environmental Impact of Unchecked Use and the Potential of Biocontrol Agents as an Eco-Friendly Alternative

“…In addition to C. michiganensis , other bacteria, such as Streptomyces sp. strain HL-12, Bacillus subtilis , Trichoderma harzianum , and Rhodosporidium diobovatum , have demonstrated antibacterial activity against Pseudomonads [ 232 , 233 ]. Notably, Staphylococcus pasteuri and Staphylococcus warneri showed promise as biocontrol agents against the bacterium Xanthomonas citri subsp.…”