General and Specific Perspectives on Biological Impacts in Wet Storage Facilities for Irradiated Nuclear Fuel

Johnson, A.B.; Burke, Sean

doi:10.1007/978-94-011-5792-6_12

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…Due to the difficulties imposed by radiation on investigation of the biodeterioration of materials in nuclear fuel storage facilities, there is a lack of information in this field [20]. Thus, it is important to identify opportunities to pursue research in this area.…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of bacteria from spent nuclear fuel pools

Chicote

García

Moreno

et al. 2005

J IND MICROBIOL BIOTECHNOL

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The aim of the present research was to isolate and identify bacteria from spent nuclear fuel pools of a Spanish nuclear power plant. Water samples were collected and inoculated onto different culture media to isolate the highest number of species. 16S rDNA fragments from colonies growing on solid media were amplified and analysed by denaturing gradient gel electrophoresis. Sequencing revealed the presence of 21 different bacteria belonging to several phylogenetic groups (alpha, beta, and gamma-Proteobacteria, Actinomycetales, Flavobacterium, and the Bacillus/Staphylococcus group). The isolation of these microorganisms in this particular environment (oligotrophic and radioactive) is highly interesting because of the possibility of their being used for the bioremediation of radionuclide-contaminated waters.

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Section: Discussionmentioning

confidence: 99%

Isolation and identification of bacteria from spent nuclear fuel pools

Chicote

García

Moreno

et al. 2005

J IND MICROBIOL BIOTECHNOL

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“…For security and economic reasons, the water temperature in SNF storage systems is often set to above room temperature (>25°C) during normal operation. Such elevated water temperatures have in some cases increased biofouling (Johnson and Burke 1997).…”

Section: Introductionmentioning

confidence: 99%

Identification of Meiothermus as the dominant genus in a storage system for spent nuclear fuel

2005

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Aims: To characterize the biofilms in an interim wet storage system (CLAB) for spent nuclear fuel. Methods and Results: Planktonic cells and biofilms were analysed with fluorescence microscopy, and scanning and transmission electron microscopy. The organisms in the biofilms were filamentous, consisting of sheaths with series of rod-shaped cells in them. Planktonic cell populations ranged between 1AE4 · 10 3 and 5AE2 · 10 3 ml )1 , correlated with the system configuration, and was inversely correlated with total organic carbon (TOC) levels. Analysis of 16S rDNA indicated that a potentially novel Meiothermus sp. was dominant in the CLAB biofilms. A Meiothermus-specific probe was designed, and statistical analysis of fluorescence in situ hybridization results confirmed that Meiothermus sp. composed up to 98% of the biofilm. The low TOC levels (2-55 lg l )1 ) in the system combined with elevated water temperatures (ca 36°C) mimic the natural environments of Meiothermus sp. as well as the recommended conditions for isolating Meiothermus sp., thus supporting the results of the 16S rDNA analysis. Conclusions: A possibly novel Meiothermus sp. is the predominant genus found in the CLAB biofilms. Significance and Impact of the Study: TOC levels are often kept low to protect industrial systems from biofouling. However, the present study shows that, rather than protecting industrial systems against biofouling per se, maintaining low TOC levels might still result in biofilms, dominated by new, unknown bacterial species.

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“…for new pipeline construction, 34 and considered as maintenance costs in others. In some activities, high degrees of responsibility and/or potential hazard are involved, such as: the nuclear industry, where Microbiologically Influenced Corrosion (MIC) 35 seriously threatens the safe, long term storage of nuclear waste; 36 the possibility of fire, explosion or loss of lives in the petrochemical industry; or in medicine, with respect to biomaterials. In such cases, the choice of materials should not simply be based upon which one offers the greatest economic advantages.…”

Section: Anticorrosion Policymentioning

confidence: 99%

Methodology to study cost of corrosion

Biezma¹,

Cristóbal²

2005

Corrosion Engineering, Science and Technology

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It is essential to have an in-depth understanding of corrosion phenomena, from both a scientific and a technological viewpoint, in order to develop strategies that minimise the costs associated with the corrosion of materials. Looked at in this way, corrosion can be seen to be not merely an economic problem. Studies of the Cost of Corrosion have been undertaken in several countries, including Japan, UK, USA, and have estimated losses to the national economy due to corrosion of up to 5% of Gross Domestic Product (GDP). This high cost stems directly from a general lack of awareness of the economic impact of corrosion, and the poor selection of protection measures. Although a definitive method to determine corrosion costs has not been established, the main methods that have been applied are the Uhlig method, which calculates corrosion related costs based on corrosion prevention methods; the Hoar method, which makes estimates in various industrial sectors, and the Input/Output method, based on an Input/Output matrix. The present study summarises the methods that have been widely used, and the results obtained in each one of the countries in which they have been applied. On this basis, a programme is proposed to estimate the costs of corrosion associated with several important industrial activities.

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General and Specific Perspectives on Biological Impacts in Wet Storage Facilities for Irradiated Nuclear Fuel

Cited by 3 publications

References 5 publications

Isolation and identification of bacteria from spent nuclear fuel pools

Isolation and identification of bacteria from spent nuclear fuel pools

Identification of Meiothermus as the dominant genus in a storage system for spent nuclear fuel

Methodology to study cost of corrosion

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