Gene Transfer to Plants: Assessment of Published Approaches and Results

Potrykus, Ingo

doi:10.1146/annurev.pp.42.060191.001225

Cited by 338 publications

(61 citation statements)

References 55 publications

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“…The transfer of defined DNA segments into plant cells is an established procedure to study the regulation of gene expression (for review see Kuhlemeier eta/., 1987;Potrykus, 1991). Techniques for direct DNA transfer and transient expression analysis of introduced genes provide a convenient alternative to stable transformation procedures.…”

Section: Introductionmentioning

confidence: 99%

Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression

1994

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Summary An improved protocol is reported to isolate and transiently transform mesophyll protoplasts of Arabidopsis thaliana. Transfected leaf protoplasts support high levels of expression of the bacterial reporter gene coding for β‐glucuronidase (GUS), under the control of the cauliflower mosaic virus (CaMV) 35S promoter. Transient expression of GUS activity was monitored spectrophotometrically and reached a maximum between 18 and 48 h after polyethylene glycol (PEG)‐mediated DNA uptake. Histochemical staining for GUS activity revealed reproducible transformation frequencies between 40 and 60%, based on the number of protoplasts survived. To demonstrate the applicability of the transient expression system, the subcellular localization of GUS proteins tagged with different nuclear polypeptides was studied in transfected mesophyll protoplasts, revealing nuclear compartmentalization of the chimeric GUS enzymes. Furthermore, Arabidopsis mesophyll protoplasts support auxin‐mediated induction of chloramphenicol acetyl‐transferase (CAT) activity when transfected with a transcriptional fusion between the CAT reporter gene and the early auxin‐inducible PS‐IAA4/5 promoter. Hence, the method allows in vivo analysis of promoter activity and subcellular localization of fusion proteins in a homologous transformation system.

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Section: Introductionmentioning

confidence: 99%

Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression

1994

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“…Genetic manipulation techniques such as direct gene transfer and particle gun bombardment may become appropriate, alternative tools for the genetic improvement of monocotyledons including Allium species (Vasil 1987(Vasil , 1988Novfik 1990;Potrykus 1991). However, efficient regeneration of plants from in vitro cultures is a major prerequisite for the successful application of these techniques.…”

Section: Introductionmentioning

confidence: 99%

High frequency somatic embryogenesis and plant regeneration from zygotic embryo-derived callus cultures of three Allium species

Valk¹,

Scholten²,

Verstappen³

et al. 1992

Plant Cell Tiss Organ Cult

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High frequency somatic embryogenesis and plant regeneration from zygotic embryo-derived callus cultures of three Allium species Valk, P. van der; Scholten, O.E.; Verstappen, F.; Jansen, Ritsert; Dons, J.J.M. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. The plant regeneration ability of zygotic embryo-derived callus cultures was studied for 12 A. cepa varieties and accessions, two A. fistulosum varieties, one A. fistulosum x A. cepa interspecific hybrid and two A. porrum varieties. Compact embryogenic callus was induced on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid. The embryogenic calluses of all three Allium species were similar in appearance. For all accessions tested plants could be regenerated at a high frequency from this compact callus through somatic embryogenesis, when using kinetin supplemented MS medium (regeneration medium). Addition of abscisic acid to the regeneration medium stimulated the formation of both somatic embryos and shoots for a number of varieties. Concerning shoot regeneration from callus cultures, significant differences existed between genotypes of all accessions except one.Abbreviations: 2,4-D-2,4-dichlorophenoxyacetic acid, VDH-Van Der Have Seed company

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“…The technique of development of transgenic plants should be accessible to conventional plant breeders, so that they can use them as a routine technique in plant improvement programmes. Numerous gene transfer techniques have been developed in the last three decades (Potrykus 1990a(Potrykus , 1991Christou 1995;Wright 1999, Darbani et al 2008) for introduction of agronomically useful genes into crop plants leading to the large-scale development of transgenic plants and their commercialization (Dunwell 2000;Fernandez-Cornejo 2005). Ever since the first report of successful genetic transformation in plants in 1980's (Herrera Estrella et al 1983Horsch et al 1983) plant biologists are on the lookout for quicker, more efficient and simpler techniques to recover fertile transgenic plants.…”

Section: Abstract Pollen Grains Transformation Transgenic Plantsmentioning

confidence: 99%

Pollen grains as a target for introduction of foreign genes into plants: an assessment

Eapen

2011

Physiol Mol Biol Plants

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Gene Transfer to Plants: Assessment of Published Approaches and Results

Cited by 338 publications

References 55 publications

Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression

Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression

High frequency somatic embryogenesis and plant regeneration from zygotic embryo-derived callus cultures of three Allium species

Pollen grains as a target for introduction of foreign genes into plants: an assessment

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