“…Immediately after injection, five electric pulses at 90V (50 ms duration and 1s interval) were delivered to the operated head using BTX electroporator with tweezer type electrodes (7 mm diameter, BTX, Holliston, MA, USA). Finally, the successfully operated pups were placed on a heat pad until they recovered full mobility and subsequently returned to their mother [10]. The operated brain was examined with fluorescence microscope 24 h after electroporation, and the frozen sections (30 lm) were prepared.…”