Gene transfer into human leukemia cell lines by electroporation: Experience with exponentially decaying and square wave pulse

Prediction and optimization of gene transfection and drug delivery by electroporation

“…We first tested the ability to predict relative levels of DNA transfection for 34 different mammalian cell types from 12 different studies [17][18][19][20][21][22][23][24][25][26][27][28] (See Materials and methods for discussion of how these predictions were made). Of greatest importance for gene therapy applications is the ability to predict the set of electroporation conditions corresponding to the maximum level of transfection.…”

“…11,[17][18][19]24,25,[27][28][29][30][31][32][33][34][35][36][37][38] To validate the correlation, we compared experimental data to predictions of electroporation conditions that yield 50% viability, since this criterion is often used as a 'rule of thumb' to crudely identify optimal laboratory transfection conditions. [1][2][3][4] Overall, predictions for 50% viability were within an average of 29 ± 28% of experimentally determined values (eg Figure 2a,c).…”

Genetic modification of hematopoietic stem cells with nonviral systems: past progress and future prospects

“…68,69 Electroporation is an efficient way to introduce genes into practically all kinds of cells, even into cells that are 'refractory' to transfection by chemical methods [70][71][72] and has been used lately for several gene transfer applications both ex vivo and in vivo. 73 There is substantial evidence that electroporation is an efficient and reproducible method for gene transfer into hematopoietic cell lines [74][75] as well as primary hematopoietic progenitor cells [76][77][78] and their progeny.…”

Enhancement of biomolecule transport by electroporation: A review of theory and practical application to transformation of Corynebacterium glutamicum

“…Square-wave pulses are more efficient than exponential decay pulses because cells may be exposed to permeabilizing electric fields for a sufficient time without being subjected to harmful, too high, electric field strengths. In fact, it is now well established, both theoretically and experimentally, that square-wave pulses provide in vitro experimental conditions resulting in levels of electropermeabilized cell survival that cannot be reached using exponentially decaying pulses (Takahasi et al, 1991). Sample cuvette design and electrode geometry also play an important role in electroporation efficiency.…”