Gene transfer into bovine cells and embryos using replication‐defective retroviral vectors encapsidated with xenotropic murine leukemia virus envelopes

Kim, T.; Leibfried-Rutledge, ML; First, N. L.

doi:10.1080/10495399309525786

Cited by 4 publications

(1 citation statement)

References 40 publications

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“…Recent studies placed retroviral packaging cells into the perivitelline space of bovine embryos and obtained several mosaic transgenic fetuses, but no live animals were produced (4). In general, the replication-defective retroviral vectors have seen only limited use in transduction of embryos (4)(5)(6), and no transgenic farm animals have been produced.…”

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confidence: 99%

Transgenic cattle produced by reverse-transcribed gene transfer in oocytes

Chan

Homan

Ballou

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

156

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A critical requirement for integration of retroviruses, other than HIV and possibly related lentiviruses, is the breakdown of the nuclear envelope during mitosis. Nuclear envelope breakdown occurs during mitotic M-phase, the envelope reforming immediately after cell division, thereby permitting the translocation of the retroviral preintegration complex into the nucleus and enabling integration to proceed. In the oocyte, during metaphase II (MII) of the second meiosis, the nuclear envelope is also absent and the oocyte remains in MII arrest for a much longer period of time compared with M-phase in a somatic cell. Pseudotyped replication-defective retroviral vector was injected into the perivitelline space of bovine oocytes during MII. We show that reverse-transcribed gene transfer can take place in an oocyte in MII arrest of meiosis, leading to production of offspring, the majority of which are transgenic. We discuss the implications of this mechanism both as a means of production of transgenic livestock and as a model for naturally occurring recursive transgenesis.

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confidence: 99%