Retrovirus-Mediated Gene Transfer

Koo, Bon Chul; Kwon, Mo Sun; Kim, Teoan

doi:10.1016/b978-0-12-410490-7.00006-2

Cited by 27 publications

(4 citation statements)

References 98 publications

(58 reference statements)

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“…The transfection medium mixture was replaced with fresh culture medium after 8 hr post-transfection, and then the culture medium containing the viruses packaged with vesicular stomatitis virus G glycoprotein (VSV-G) was collected at 48 hr post-transfection. To prepare the virus solution for microinjection, the medium taken from the virus-producing cells was centrifugally concentrated 1,000-fold and sterilized by filtering through a 0.45-μm pore-size filter [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Generation of transgenic chickens expressing the human erythropoietin (hEPO) gene in an oviduct-specific manner: Production of transgenic chicken eggs containing human erythropoietin in egg whites

et al. 2018

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The transgenic chicken has been considered as a prospective bioreactor for large-scale production of costly pharmaceutical proteins. In the present study, we report successful generation of transgenic hens that lay eggs containing a high concentration of human erythropoietin (hEPO) in the ovalbumin. Using a feline immunodeficiency virus (FIV)-based pseudotyped lentivirus vector enveloped with G glycoproteins of the vesicular stomatitis virus, the replication-defective vector virus carrying the hEPO gene under the control of the chicken ovalbumin promoter was microinjected to the subgerminal cavity of freshly laid chicken eggs (stage X). Stable germline transmission of the hEPO transgene to the G1 progeny, which were non-mosaic and hemizygous for the hEPO gene under the ovalbumin promoter, was confirmed by mating of a G0 rooster with non-transgenic hens. Quantitative analysis of hEPO in the egg whites and in the blood samples taken from G1 transgenic chickens showed 4,810 ~ 6,600 IU/ml (40.1 ~ 55.0 μg/ml) and almost no detectable concentration, respectively, indicating tightly regulated oviduct-specific expression of the hEPO transgene. In terms of biological activity, there was no difference between the recombinant hEPO contained in the transgenic egg white and the commercially available counterpart, in vitro. We suggest that these results imply an important step toward efficient production of human cytokines from a transgenic animal bioreactor.

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Section: Methodsmentioning

confidence: 99%

Generation of transgenic chickens expressing the human erythropoietin (hEPO) gene in an oviduct-specific manner: Production of transgenic chicken eggs containing human erythropoietin in egg whites

et al. 2018

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“…Pro encodes the protease (PR) that processes the gag precursor. Env encodes the surface glycoprotein (SU) and transmembrane (TM) domains of the viral envelope that associate with specific cellular receptors and mediates fusion with the cell membrane ( Koo et al, 2014 ).…”

Section: Biological Vectorsmentioning

confidence: 99%

Designing molecules: directing stem cell differentiation

Thanaskody,

Natashah,

Nordin

et al. 2024

Front. Bioeng. Biotechnol.

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Stem cells have been widely applied in regenerative and therapeutic medicine for their unique regenerative properties. Although much research has shown their potential, it remains tricky in directing stem cell differentiation. The advancement of genetic and therapeutic technologies, however, has facilitated this issue through development of design molecules. These molecules are designed to overcome the drawbacks previously faced, such as unexpected differentiation outcomes and insufficient migration of endogenous or exogenous MSCs. Here, we introduced aptamer, bacteriophage, and biological vectors as design molecules and described their characteristics. The methods of designing/developing discussed include various Systematic Evolution of Ligands by Exponential Enrichment (SELEX) procedures, in silico approaches, and non-SELEX methods for aptamers, and genetic engineering methods such as homologous recombination, Bacteriophage Recombineering of Electroporated DNA (BRED), Bacteriophage Recombineering with Infectious Particles (BRIP), and genome rebooting for bacteriophage. For biological vectors, methods such as alternate splicing, multiple promoters, internal ribosomal entry site, CRISPR-Cas9 system and Cre recombinase mediated recombination were used to design viral vectors, while non-viral vectors like exosomes are generated through parental cell-based direct engineering. Besides that, we also discussed the pros and cons, and applications of each design molecule in directing stem cell differentiation to illustrate their great potential in stem cells research. Finally, we highlighted some safety and efficacy concerns to be considered for future studies.

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“…Retroviruses are used as vectors for gene transfer. These vectors efficiently transfer genes due to their affinity and infectivity for specific targeted cell and will result in the successful incorporation of transgene [88]. The genetic material is transferred in the form of RNA into the host cells resulting in the chimera animal, these animals are then subjected to inbred for about 20 generations to produce homozygous transgenic animals which carries desired transgene in each and every cell of these offspring's [89].…”

Section: Retrovirus-mediated Gene Transfermentioning

confidence: 99%

Innovative Reproductive Technology in Animal Breeding: A Review

Dhangada,

Gaikwad,

Dhage

et al. 2024

J. Adv. Biol. Biotechnol.

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The profitability and the intensity achieved by the genetic improvement is determined by the reproductive performance of the farm animals. The major cause of economic loss is reproductive inefficiency in the livestock sector. In the past few years, several advances have been made in reproductive technologies (RTs) to increase the efficiency of genetic improvement. These technologies include Artificial Insemination, Sexed semen Technology, Cryopreservation, Multiple Ovulation and Embryo Transfer, In-vitro Embryo Production (IVEP), Transgenesis etc. The problems of infertility of male or female, postpartum infertility in high-yielding animals of high genetic merit individuals and breeding of distant species has been overcome through these technologies. The inclusion of these techniques in animal breeding had made possible to obtain large number of progenies from genetically superior animals. This review, discuss the various innovative reproductive technologies implemented in livestock sector.

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Retrovirus-Mediated Gene Transfer

Cited by 27 publications

References 98 publications

Generation of transgenic chickens expressing the human erythropoietin (hEPO) gene in an oviduct-specific manner: Production of transgenic chicken eggs containing human erythropoietin in egg whites

Generation of transgenic chickens expressing the human erythropoietin (hEPO) gene in an oviduct-specific manner: Production of transgenic chicken eggs containing human erythropoietin in egg whites

Designing molecules: directing stem cell differentiation

Innovative Reproductive Technology in Animal Breeding: A Review

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