Gene Therapy of Bone Morphogenetic Protein for Periodontal Tissue Engineering

Qm, Jin; Anusaksathien, Orasa; Sa, Webb; Rb, Rutherford; Giannobile, William V.

doi:10.1902/jop.2003.74.2.202

Cited by 159 publications

(154 citation statements)

References 53 publications

(56 reference statements)

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“…This phenomenon has been noted in rat osteoblasts, the mesodermal pluripotent cell line C1, and in BMP-induced skeletogenesis [23,24]. More recently, when dermal fibroblasts were transduced with Ad-BMP-7 and then transplanted to periodontal defects in rats, these cells not only expressed BMP-7 genes and corresponding protein, but also increased gene and protein expression of noggin [11]. Talwar et al reported that both rapid and slow release of BMP did not contribute to the union of bone to the root surface [25].…”

Section: Discussionmentioning

confidence: 73%

“…BMP-2 triggers the differentiation of dental follicle cells into cementoblasts or osteoblasts in vitro [8]. Moreover, BMPspotently stimulate alveolar bone regeneration around teeth [9], as well as initiate cementogenesis in periodontal wounds [10,11]. Therefore, BMP signaling appears to be critical in regulating periodontal tissue formation in pre-and postnatal life.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to the requirement of noggin for normal skeletogenesis [14], noggin plays an important role in tooth development, because application of noggin to embryonic tooth bud explants can transform tooth type from incisors to molars [15]. Furthermore, noggin gene transfer to periodontal wounds has been shown to inhibit alveolar bone repair [11].…”

Section: Introductionmentioning

confidence: 99%

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Noggin Gene Delivery Inhibits Cementoblast-Induced Mineralization

Jin

Zhao

Economides

et al. 2004

Connective Tissue Research

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Bone morphogenetic proteins (BMPs) are known to promote periodontal tissue regeneration, while noggin inhibits the biological activities of BMP-2, -4, and -7. To investigate the effect of BMPs and noggin gene transfer on cementogenesis,we used cloned murine cementoblasts (OCCM). Cells were transduced using adenoviruses encoding BMP-7 (Ad-BMP-7), noggin devoid of the heparin binding site (Ad-NOGΔB2), or a control adenovirus encoding green fluorescent protein (Ad-GFP). Cells were seeded into 3D polymer scaffolds and implanted into SCID mice to determine the in vivo mineral-inducing ability of the cells. Cells transduced with Ad-NOG.B2 at 3 and 6 weeks postimplantation exhibited reduced mineral formation compared with all other groups. Although gene expression of osteocalcin and bone sialoprotein increased after Ad-BMP-7 transduction in vitro, following BMP-7 gene transfer in vivo, transcripts for OCN and BSP were not significantly different from controls, and mineral density was not significantly increased compared with Ad-GFP and NT groups. These results indicate that in mature cementoblast populations, gene transfer of noggin inhibits biomineralization induced by cementoblasts, whereas exogenous BMP has minimal effects on mineralization.

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Section: Discussionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Noggin Gene Delivery Inhibits Cementoblast-Induced Mineralization

Jin

Zhao

Economides

et al. 2004

Connective Tissue Research

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“…A long time ago, it was shown that PDGF molecules were able to stimulate periodontal healing and regeneration [35,36]. Since then, other molecules, such as BMPs [37][38][39] and amelogenins [40], have been used for the stimulation of periodontal tissue regeneration.…”

Section: Stem Cell-based Dental Tissue Regenerationmentioning

confidence: 99%

Nanodentistry: Combining Nanostructured Materials and Stem Cells for Dental Tissue Regeneration

2012

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Regenerative dentistry represents an attractive multidisciplinary therapeutic approach that complements traditional restorative/surgery techniques and benefits from recent advances in stem cell biology, molecular biology, genomics and proteomics. Materials science is important in such advances to move regenerative dentistry from the laboratory to the clinic. The design of novel nanostructured materials, such as biomimetic matrices and scaffolds for controlling cell fate and differentiation, and nanoparticles for diagnostics, imaging and targeted treatment, is needed. The combination of nanotechnology, which allows the creation of sophisticated materials with exquisite fine structural detail, and stem cell biology turns out to be increasingly useful in regenerative medicine. The administration to patients of dynamic biological agents comprising stem cells, bioactive scaffolds and/or nanoparticles will certainly increase the regenerative impact of dental pathological tissues. This overview briefly describes some of the actual benefits and future possibilities of nanomaterials in the emerging field of stem cell-based regenerative dentistry.

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“…9 Investigation of the effectiveness of this potential periodontal treatment strategy using ex vivo gene transfer of BMP-7 through syngeneic dermal fibroblasts yielded successful results. 10 Adult bone marrow mesenchymal stem cells (MSCs) can be engineered to express an assortment of factors in vivo; moreover, they have been demonstrated to respond to these factors and differentiate into skeletal specialized cells. 11 This allows them to actively contribute to periodontal tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%