Gene targeting in a nonhomologous end joining deficient Hypocrea jecorina

Zhang, Guangtao; Hartl, Lukas; Schuster, André; Polák, Štefan; Schmoll, Monika; Wang, Tianhong; Seidl, Verena; Schink, Bernhard

doi:10.1016/j.jbiotec.2008.10.007

Cited by 130 publications

(119 citation statements)

References 29 publications

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“…Deletion of the lxr4 Gene in T. reesei-A T. reesei ⌬tku70 strain was used as the parental strain for transformation (14,15). The cassette for deletion of the lxr4 gene (GenBank TM accession number BK008566) contained 937 bp of the promoter region, 1162 bp of the terminator region, and a fragment containing the pyr4 encoding orotidine-5Ј-phosphate decarboxylase.…”

Section: Methodsmentioning

confidence: 99%

l-xylo-3-Hexulose Reductase Is the Missing Link in the Oxidoreductive Pathway for d-Galactose Catabolism in Filamentous Fungi

Mojžita

Herold

Metz

et al. 2012

Journal of Biological Chemistry

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Background:There is an oxidoreductive D-galactose pathway in filamentous fungi. Results:We identified an L-xylo-3-hexulose reductase that produces D-sorbitol and that is part of this pathway. Conclusion: This L-xylo-3-hexulose reductase is the missing link in the oxidoreductive D-galactose pathway. Significance: The alternative pathway for D-galactose catabolism in filamentous fungi is elucidated.

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Section: Methodsmentioning

confidence: 99%

l-xylo-3-Hexulose Reductase Is the Missing Link in the Oxidoreductive Pathway for d-Galactose Catabolism in Filamentous Fungi

Mojžita

Herold

Metz

et al. 2012

Journal of Biological Chemistry

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“…NHEJ repair has evolved as the predominant pathway in filamentous fungi, as in other superior eukaryotes, such as plants and mammals (106). In T. reesei as well as in other filamentous fungi, some components of the NHEJ pathway (mus53/lig4; TR_58509, TA_83609, and TV_180472 and ku70 homologs) have been the target of deletion in order to increase the proportion of homologous recombination events upon transformation for targeted gene replacement experiments (107)(108)(109). Homologs of all genes involved in NHEJ are present in the genomes of the Trichoderma species analyzed here (see Table S1 in the supplemental material).…”

Section: Dna Repairmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

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SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

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“…Recent advances in method development have resulted in high-throughput generation of knockout/gene replacement strains by more efficient strategies, increasing the efficiency of homologous recombination and allowing sequential gene deletions. While these strategies have so far been applied to T. reesei QM9414-derived strains (Hartl & Seiboth, 2005;Guangtao et al, 2009) and the wild-type QM6a (Steiger et al, 2011), they could similarly be applied to the RUT-C30 strain. In addition, alternative strategies such as gene knock-down by mRNA silencing (reviewed by Nakayashiki et al, 2005) could be employed for partial or condition-specific suppression of gene expression, particularly when gene deletion could be detrimental or even lethal.…”

Section: Rut-c30 As a Host For Heterologous Protein Productionmentioning

confidence: 99%

Trichoderma reesei RUT-C30 – thirty years of strain improvement

2012

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The hypersecreting mutant Trichoderma reesei RUT-C30 (ATCC 56765) is one of the most widely used strains of filamentous fungi for the production of cellulolytic enzymes and recombinant proteins, and for academic research. The strain was obtained after three rounds of random mutagenesis of the wild-type QM6a in a screening program focused on high cellulase production and catabolite derepression. Whereas RUT-C30 achieves outstanding levels of protein secretion and high cellulolytic activity in comparison to the wild-type QM6a, recombinant protein production has been less successful. Here, we bring together and discuss the results from biochemical-, microscopic-, genomic-, transcriptomic-, glycomic-and proteomic-based research on the RUT-C30 strain published over the last 30 years.

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Gene targeting in a nonhomologous end joining deficient Hypocrea jecorina

Cited by 130 publications

References 29 publications

l-xylo-3-Hexulose Reductase Is the Missing Link in the Oxidoreductive Pathway for d-Galactose Catabolism in Filamentous Fungi

l-xylo-3-Hexulose Reductase Is the Missing Link in the Oxidoreductive Pathway for d-Galactose Catabolism in Filamentous Fungi

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Trichoderma reesei RUT-C30 – thirty years of strain improvement

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