Trichoderma reesei RUT-C30 – thirty years of strain improvement

Abstract

The hypersecreting mutant Trichoderma reesei RUT-C30 (ATCC 56765) is one of the most widely used strains of filamentous fungi for the production of cellulolytic enzymes and recombinant proteins, and for academic research. The strain was obtained after three rounds of random mutagenesis of the wild-type QM6a in a screening program focused on high cellulase production and catabolite derepression. Whereas RUT-C30 achieves outstanding levels of protein secretion and high cellulolytic activity in comparison to the wild-type QM6a, recombinant protein production has been less successful. Here, we bring together and discuss the results from biochemical-, microscopic-, genomic-, transcriptomic-, glycomic-and proteomic-based research on the RUT-C30 strain published over the last 30 years.

Evaluation of 18 isolates of basidiomycetes for Lignocellulose degrading enzymes

“…Pycnoporus sp. produced the highest cellulase activity of 0.23 FPU/ml, which is comparable to the activities produced by the wild type filamentous fungus Trichoderma reesei QM6a (Mandels, 1975), which is the parent strain of the mutant strain Trichoderma reesei RUT-C30, used for industrial production of cellulases (Peterson and and Nevalainen, 2012). The highest xylanase activity of 5.4 U/ml was produced by Phlebiopsis sp.…”

Obtaining New Cultures Of Microorganisms That Produces Cellulases And Xylanases From The Sugarcane Bagasse

“…Table 2 Maximum values of volumetric productivity observed for production of cellulases by T. harzianum L04 strain grown on different carbon sources analyzed in this work, L04 showed a shorter acclimation period when cultivated in a complex substrate such as SCB. This observation is particularly interesting when compared to T. reesei Rut C30, a widely used filamentous fungus strain used for the production of cellulolytic enzymes (Peterson and Nevalainen 2012). T. reesei Rut C30 shows a longer acclimation to lignocellulosic feedstock and is known to have a better performance for cellulase production when grown on pure cellulosic substrates then on lignocellulosic ones (Juhász et al 2005), data confirmed by Castro et al (2010c) where maximum values of volumetric productivity for T. reesei Rut C30 were obtained at 333 hours culture.…”

Bacterial genomes: what they teach us about cellulose degradation

“…This may be result of a bias in isolation or sequencing of cellulolytic organisms, or by a lower number of Gram-negative cellulolytic organisms in the environment. No Gram-negative organism possesses a cellulolytic set of enzymes comparable to either the Gram-positive sets of cellulases described above or the fungal set described for T. reesei [7,59]. Of the Gramnegatives, Cellvibrio japonicus appears to have the most 'normal' collection of cellulases (Supplementary Table M).…”