Gene structure and evolution of Tieg3, a new member of the Tieg family of proteins

Wang, Ziyuan; Peters, Björn; Klussmann, Silke; Bender, Herdis; Herb, Anne; Krieglstein, Kerstin

doi:10.1016/j.gene.2003.09.045

Cited by 21 publications

(35 citation statements)

References 33 publications

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“…They also showed evidence that overexpression of TIEG2 in Chinese hamster ovary cells inhibits cell proliferation. Recently, Wang et al (36) identified another member of the TIEG family, TIEG3, which has properties similar to those of TIEG1 and TIEG2.…”

mentioning

confidence: 99%

TIEG1 Null Mouse-Derived Osteoblasts Are Defective in Mineralization and in Support of Osteoclast Differentiation In Vitro

Subramaniam¹,

Gorny²,

Johnsen

et al. 2005

Molecular and Cellular Biology

109

129

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Transforming growth factor ␤-inducible early gene 1 (TIEG1) is a member of the Krüppel-like transcription factor family. To understand the physiological role of TIEG1, we generated TIEG ؊/؊ (null) mice and found that the TIEG ؊/؊ mice had increased osteoblast numbers with no increased bone formation parameters. However, when calvarial osteoblasts (OBs) were isolated from neonatal TIEG ؊/؊ and TIEG ؉/؉ mice and cultured in vitro, the TIEG ؊/؊ cells displayed reduced expression of important OB differentiation markers. When the OBs were differentiated in vitro by treatment with bone morphogenic protein 2, the OBs from TIEG ؉/؉ calvaria displayed several mineralized nodules in culture, whereas those from TIEG ؊/؊ mice showed no nodules. To characterize the OBs' ability to support osteoclast differentiation, the OBs from TIEG ؉/؉ and TIEG ؊/؊ mice were cultured with marrow and spleen cells from TIEG ؉/؉ mice. Significantly fewer osteoclasts developed when TIEG ؊/؊ OBs were used to support osteoclast differentiation than when TIEG ؉/؉ OBs were used. Examination of gene expression in the TIEG ؊/؊ OBs revealed decreased RANKL and increased OPG expression compared to TIEG ؉/؉ OBs. The addition of RANKL to these cocultures only partially restored the ability of TIEG ؊/؊ OBs to support osteoclast differentiation, whereas M-CSF alone or combined with RANKL had no additional effect on osteoclast differentiation. We conclude from these data that TIEG1 expression in OBs is critical for both osteoblast-mediated mineralization and osteoblast support of osteoclast differentiation.Krüppel-like transcription factors (KLFs) are DNA-binding transcriptional regulators which contain C 2 , H 2 -type zinc fingers and play important roles in regulating biological processes such as cell growth, differentiation, and embryogenesis (1, 5, 32). The number of members of the KLF family has been increasing, and it is estimated that 1% of the human genome might contain this family of regulatory factors (5, 11). Our laboratory has cloned a member of this family, the transforming growth factor ␤ (TGF-␤)-inducible early gene 1 (TIEG1), since it represented a primary response gene to TGF-␤ treatment in human osteoblasts (28). Cook et al. (4) identified TIEG2, which shares 91% homology with TIEG1 within the zinc finger region but only 44% homology at the N terminus region. They also showed evidence that overexpression of TIEG2 in Chinese hamster ovary cells inhibits cell proliferation. Recently, Wang et al. (36) identified another member of the TIEG family, TIEG3, which has properties similar to those of TIEG1 and TIEG2.A better understanding of the mechanism of action of TIEG1 is evolving. Using a GAL4-based transcriptional assay, Cook et al. (4) demonstrated that TIEG1 protein has three repression domains. Studies by Zhang et al. (39) identified an alpha-helical repression motif located within the repression domain of TIEG1 and TIEG2. These authors have also shown evidence that these motifs mediate the direct interaction of TIEG1 with mSin3A, whic...

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confidence: 99%

TIEG1 Null Mouse-Derived Osteoblasts Are Defective in Mineralization and in Support of Osteoclast Differentiation In Vitro

Subramaniam¹,

Gorny²,

Johnsen

et al. 2005

Molecular and Cellular Biology

109

129

View full text Add to dashboard Cite

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“…TGFβ and members of the TGFβ/ Smad signaling pathway share similar anti-proliferative properties and function as suppressors of growth (Cook et al, 1998;Cook and Urrutia, 2000). TIEG was discovered and characterized as a three-zinc finger containing nuclear, transcription factor activated in response to TGFβ in human osteoblasts (Wang et al, 2004;Cook et al, 1999;Subramaniam et al, 1995). TIEG is a Krüppel-like transcriptional factor that serves a unique regulatory role in the TGFβ signal transduction pathway.…”

Section: Resultsmentioning

confidence: 99%

Genetic networks of cooperative redox regulation of osteopontin

Partridge

Brun

et al. 2008

Matrix Biology

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“…However, the serine-rich region found at the amino terminus of the D. melanogaster Cbt protein was not identified in any of them. Conversely, they contain a proline-rich region (Subramaniam et al 1995;Yajima et al 1997;Cook et al 1998;Wang et al 2004; data not shown), which may associate with the SH3 domains of src tyrosine kinases and be involved in signal transduction processes (Subramaniam et al 1995;Yajima et al 1997;Ellenrieder et al 2002). Pair-wise comparisons with the D. melanogaster Cbt protein revealed that the percentages of similarity are higher when only the zinc finger domains are compared (Table 2), as seen in invertebrates, suggesting that this region may play an important role in Cbt function.…”

Section: Analysis Of Cabut Orthologs In Vertebratesmentioning

confidence: 96%

“…These proteins Sequence accession numbers: human TIEG1, AF050110; human TIEG2, NM_003597; chimpanzee TIEG1, XM_528205; chimpanzee TIEG2, XM_515296; mouse TIEG1, NM_013692; mouse TIEG3, NM_178357; rat TIEG1, NM_031135; rat TIEG2, NM_001037354; zebrafish TIEG2, genomic contig BX248136; zebrafish TIEG3, XM_682384; frog sequence, BC121242 belong to the subgroup III of the Sp1-like/Krüppel-like (Sp1-/KLF) family of transcription factors (Subramaniam et al 1995;Cook et al 1998;Kaczynski et al 2003). In mice, three TIEG genes were initially described (TIEG1-3; Yajima et al 1997;Scohy et al 2000;Wang et al 2004). However, recent studies have suggested that murine TIEG2 could represent the human TIEG2 sequence, and therefore, this gene was not included in this study (Suske et al 2005;K.…”

Section: Analysis Of Cabut Orthologs In Vertebratesmentioning

confidence: 99%

“…Moreover, we have determined that the putative cbt orthologs in the species of vertebrates analyzed belong to the TIEG family of transcription factors. These proteins behave as cell growth repressors with antiproliferative and apoptosis-inducing functions in humans (Cook et al 1999;Ellenrieder et al 2002), and it seems that TIEG proteins also function as transcriptional repressors in mice (Yajima et al 1997;Wang et al 2004). …”

Section: Introductionmentioning

confidence: 99%

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Identification and analysis of cabut orthologs in invertebrates and vertebrates

2007

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Cabut (cbt) is a Drosophila melanogaster gene involved in epidermal dorsal closure (DC). Its expression is dependent on the Jun N-terminal kinase (JNK) cascade, and it functions downstream of Jun regulating dpp expression in the leading edge cells. The Cbt protein contains three C(2)H(2)-type zinc fingers and a serine-rich domain, suggesting that it functions as a transcription factor. We have identified single cbt orthologs in other Drosophila species, as well as in other insects and invertebrate organisms like ascidians and echinoderms, but not in nematodes. Gene structure and protein sequence are highly conserved among Drosophilidae, but are more diverged in the other species of invertebrates analyzed. According to this, we demonstrate that cbt expression is detected in the embryonic lateral epidermis in several Drosophila species, as it occurs in D. melanogaster, thus suggesting that the cbt orthologs may have a conserved role in these species during DC. We have also analyzed the genomes of several vertebrate species, finding that the cbt orthologous genes in these organisms encode proteins that belong to the TIEG family of Sp1-like/Krüppel-like transcription factors. Phylogenetic analysis of the invertebrate and vertebrate proteins identified indicates that they mainly follow the expected phylogeny of the species, and that the cbt gene was duplicated during vertebrate evolution. Because we were not able to identify cbt orthologous genes neither in yeast nor in plants, our results suggest that this gene has been probably conserved throughout metazoans and that it may play a fundamental role in animal biology.

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Gene structure and evolution of Tieg3, a new member of the Tieg family of proteins

Cited by 21 publications

References 33 publications

TIEG1 Null Mouse-Derived Osteoblasts Are Defective in Mineralization and in Support of Osteoclast Differentiation In Vitro

TIEG1 Null Mouse-Derived Osteoblasts Are Defective in Mineralization and in Support of Osteoclast Differentiation In Vitro

Genetic networks of cooperative redox regulation of osteopontin

Identification and analysis of cabut orthologs in invertebrates and vertebrates

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