Gene silencing reveals multiple functions of Na+/K+-ATPase in the salmon louse (Lepeophtheirus salmonis)

Komisarczuk, Anna Z.; Kongshaug, Heidi; Nilsen, Frank

doi:10.1016/j.exppara.2018.01.005

Cited by 9 publications

(3 citation statements)

References 76 publications

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“…Numerous physiological activities, including the maintenance of membrane excitability, osmotic management, cell volume regulation, and the transfer of nutrients, depend on Na + /K + -ATPase. 21 As shown in Figure 2E, exposure to PMPs and AMPs caused a significant decrease of the Na + /K + -ATPase activity only at 50A exposure, indicating that Na + influx was not the primary cause of intestinal cell destruction. Furthermore, the level of Ca 2+ -ATPase decreased, suggesting that intracellular Ca 2+ cannot be expelled from the cell (Figure 2F).…”

Section: Accumulation Oxidative Stress and Cell Homeostasis In Earthw...mentioning

confidence: 82%

“…The activities of Na + /K + -ATPase and Ca 2+ -ATPase, which are ion pumps that regulate the concentration of major ions in the cell, provide energy for the transport of ions and nutrients, and are crucial for the maintenance of normal cellular morphology, structure, and physiological functions, were also examined. Numerous physiological activities, including the maintenance of membrane excitability, osmotic management, cell volume regulation, and the transfer of nutrients, depend on Na + /K + -ATPase . As shown in Figure E, exposure to PMPs and AMPs caused a significant decrease of the Na + /K + -ATPase activity only at 50A exposure, indicating that Na + influx was not the primary cause of intestinal cell destruction.…”

Section: Resultsmentioning

confidence: 99%

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Microplastics - Back to Reality: Impact of Pristine and Aged Microplastics in Soil on Earthworm Eisenia fetida under Environmentally Relevant Conditions

Jiang,

Cao,

et al. 2023

Environ. Sci. Technol.

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Recently, studies have highlighted the potential danger for soil organisms posed by film-derived microplastics (MPs). However, the majority of those does not accurately reflect the field conditions and the degree of MP contamination that can be found in actual settings. To fill the gap between laboratory and field scenarios, the polyethylene (PE) plastic film was made into PE-MPs and aged. Toxicity and molecular mechanisms of pristine PE-MPs (PMPs) and aged PE-MPs (AMPs) with the concentration at 500 mg/kg of dry weight were determined after 14 days of exposure by measuring the oxidative stress, osmoregulation pressure, gut microbiota, and metabolic responses in earthworms under environmentally relevant conditions. Our research showed that, when compared to PMPs (13.13 ± 1.99 items/g), AMPs accumulated more (16.19 ± 8.47 items/g), caused more severe tissue lesions, and caused a higher increase of cell membrane osmotic pressure in earthworms' intestines. Furthermore, the proportion of probiotic bacteria Lactobacillus johnsonii in the gut bacterial communities was 24.26%, 23.26%, and 12.96%, while the proportion of pathogenic bacteria of the phylum Verrucomicrobia was 2.28%, 4.79%, and 10.39% in the control and PMP-and AMP-exposed earthworms, indicating that the decrease in number of probiotic bacteria and the increase in number of pathogenic bacteria were more pronounced in the gut of AMP-rather than PMP-exposed earthworms. Metabolomic analysis showed that AMP exposure reduced earthworm energy metabolites. Consequently, the constant need for energy may result in protein catabolism, which raises levels of some amino acids, disturbs normal cell homeostasis, causes changes of cell membrane osmolarity, and destroys the cell structure. Our studies showed that aged MPs, with the same characteristics as those found in the environment, have greater toxicity than pristine MPs. The results of this study broaden our understanding of the toxicological effects of MPs on soil organisms under environmentally relevant conditions.

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Section: Accumulation Oxidative Stress and Cell Homeostasis In Earthw...mentioning

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Microplastics - Back to Reality: Impact of Pristine and Aged Microplastics in Soil on Earthworm Eisenia fetida under Environmentally Relevant Conditions

Jiang,

Cao,

et al. 2023

Environ. Sci. Technol.

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“…RNA interference screens have aided the study of hostparasite interactions, host recognition (Komisarczuk et al, 2017;Núñez-Acuña et al, 2019), and physiology at various life stages of lice (Sandlund et al, 2016(Sandlund et al, , 2018Komisarczuk et al, 2018Komisarczuk et al, , 2020Braden et al, 2020;Guragain et al, 2020). RNAi has also facilitated in the functional studies of key genes involved in lice reproduction (Dalvin et al, 2009;Tröße et al, 2014;Eichner et al, 2015a;Khan et al, 2017;Borchel and Nilsen, 2018;Borchel et al, 2019;Heggland et al, 2019a,b).…”

Section: Rna Interferencementioning

confidence: 99%

Principles and Methods of Counteracting Harmful Salmon–Arthropod Interactions in Salmon Farming: Addressing Possibilities, Limitations, and Future Options

et al. 2021

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The arthropod salmon louse (Lepeophtheirus salmonis) is a major threat to Atlantic salmon aquaculture and wild salmonids. Essentially like in monoculture, very high concentrations of susceptible hosts may result in high reproduction and severe production of waves of pests. Pest management is crucial both for fish health and protection of wild fish populations from aquaculture influence. Various methods have been utilized to control salmon lice infestations, such as pesticide use, physical treatments, construction modifications, fallowing, breeding, vaccination, and biological control. Most of the methods are partially successful, but none completely fulfills the necessary pest control strategy. Like in agriculture, lice/pest management is an arms race, but the marine environment makes it even more difficult to precisely hit the target pest and avoid unintended negative effects on general wildlife. In this study, we provide an overview of the methods and principles of salmon lice management and address current possibilities and limitations. We also highlight the potential of emerging strategies and enabling technologies, like genome editing, RNA interference, and machine learning, in arthropod management in aquaculture.

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Na+/K+-ATPase a Primary Membrane Transporter: An Overview and Recent Advances with Special Reference to Algae

Kumari

Rathore

2020

J Membrane Biol

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Gene silencing reveals multiple functions of Na+/K+-ATPase in the salmon louse (Lepeophtheirus salmonis)

Cited by 9 publications

References 76 publications

Microplastics - Back to Reality: Impact of Pristine and Aged Microplastics in Soil on Earthworm Eisenia fetida under Environmentally Relevant Conditions

Microplastics - Back to Reality: Impact of Pristine and Aged Microplastics in Soil on Earthworm Eisenia fetida under Environmentally Relevant Conditions

Principles and Methods of Counteracting Harmful Salmon–Arthropod Interactions in Salmon Farming: Addressing Possibilities, Limitations, and Future Options

Na+/K+-ATPase a Primary Membrane Transporter: An Overview and Recent Advances with Special Reference to Algae

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