Gene repression in S. cerevisiae—looking beyond Sir-dependent gene silencing

Sauty, Safia Mahabub; Shaban, Kholoud; Yankulov, Krassimir

doi:10.1007/s00294-020-01114-7

Cited by 7 publications

(5 citation statements)

References 148 publications

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“…Gene silencing at the subtelomeric, rRNA gene or mating type loci of Saccharomyces cerevisiae is an extensively studied phenomenon and serves as a paradigm for gene repression and silencing in other eukaryotes (Rowlands et al 2017;Sauty et al 2021). Multiple earlier studies have measured the levels of gene silencing by inserting the URA3 gene in these loci.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of drug-free methods for the detection of gene silencing in Saccharomyces cerevisiae

Shaban

Sauty

Fisher

et al. 2023

Biochem. Cell Biol.

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Multiple studies in Saccharomyces cerevisiae have measured the levels of gene silencing by inserting the URA3 gene at various loci and selecting against URA3-expressing cells by 5-flouroorotic acid (5-FOA). However, 5-FOA affects the cellular pools of dNTPs and can produce side effects. To circumvent this issue, we and others have introduced drug-free techniques to detect silent and active gene states. In this study, we compared three drug-free methods based on the expression of fluorescent reporters in the VIIL telomere of S. cerevisiae. Our results point out that only one of these methods is suitable for large-scale drug-free analyses of gene silencing.

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Section: Introductionmentioning

confidence: 99%

Evaluation of drug-free methods for the detection of gene silencing in Saccharomyces cerevisiae

Shaban

Sauty

Fisher

et al. 2023

Biochem. Cell Biol.

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“…Transcription of genes of the de novo pathway is under the control of Hst1, and it conceived that this NAD-dependent deacetylase also function as an NAD sensor where it regulate NAD levels and the degree of biosynthesis of nicotinic acid gene repression. Uneven responses presented by activity of npt1 null mutants in the de novo pathway (upregulation of genes), and the salvage pathway (genes unaffected); indicate a defective NA/NAM salvage and the de novo pathway for NAD biosynthesis (Lee et al 2013 ; Sauty et al 2021 ). However, lack of Npt1 does not affect CLS differently as seen in RLS through the NA salvage pathway (Lu et al 2009 ).…”

Section: Precursors For Nad and Their Influence On Rls And Cls Lifespanmentioning

confidence: 99%

The role of NAD and NAD precursors on longevity and lifespan modulation in the budding yeast, Saccharomyces cerevisiae

et al. 2022

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Molecular causes of aging and longevity interventions have witnessed an upsurge in the last decade. The resurgent interests in the application of small molecules as potential geroprotectors and/or pharmacogenomics point to nicotinamide adenine dinucleotide (NAD) and its precursors, nicotinamide riboside, nicotinamide mononucleotide, nicotinamide, and nicotinic acid as potentially intriguing molecules. Upon supplementation, these compounds have shown to ameliorate aging related conditions and possibly prevent death in model organisms. Besides being a molecule essential in all living cells, our understanding of the mechanism of NAD metabolism and its regulation remain incomplete owing to its omnipresent nature. Here we discuss recent advances and techniques in the study of chronological lifespan (CLS) and replicative lifespan (RLS) in the model unicellular organism Saccharomyces cerevisiae . We then follow with the mechanism and biology of NAD precursors and their roles in aging and longevity. Finally, we review potential biotechnological applications through engineering of microbial lifespan, and laid perspective on the promising candidature of alternative redox compounds for extending lifespan.

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“…Besides the direct modification of DNA and histones, remodeling protein complexes play a key role in chromatin regulation [113]. The silent information regulator (SIR) complex, which consists of Sir2, Sir3, and Sir4, is involved in gene silencing on mating-type loci HML and HMR on chromosome III and regions surrounding the telomeres [114,115].…”

Section: Epigenetic Control Of Gene Expression and Yeast Life Span Ex...mentioning

confidence: 99%

Substrates of the MAPK Slt2: Shaping Yeast Cell Integrity

González-Rubio

Sastre-Vergara

Molina

et al. 2022

JoF

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The cell wall integrity (CWI) MAPK pathway of budding yeast Saccharomyces cerevisiae is specialized in responding to cell wall damage, but ongoing research shows that it participates in many other stressful conditions, suggesting that it has functional diversity. The output of this pathway is mainly driven by the activity of the MAPK Slt2, which regulates important processes for yeast physiology such as fine-tuning of signaling through the CWI and other pathways, transcriptional activation in response to cell wall damage, cell cycle, or determination of the fate of some organelles. To this end, Slt2 precisely phosphorylates protein substrates, modulating their activity, stability, protein interaction, and subcellular localization. Here, after recapitulating the methods that have been employed in the discovery of proteins phosphorylated by Slt2, we review the bona fide substrates of this MAPK and the growing set of candidates still to be confirmed. In the context of the complexity of MAPK signaling regulation, we discuss how Slt2 determines yeast cell integrity through phosphorylation of these substrates. Increasing data from large-scale analyses and the available methodological approaches pave the road to early identification of new Slt2 substrates and functions.

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Gene repression in S. cerevisiae—looking beyond Sir-dependent gene silencing

Cited by 7 publications

References 148 publications

Evaluation of drug-free methods for the detection of gene silencing in Saccharomyces cerevisiae

Evaluation of drug-free methods for the detection of gene silencing in Saccharomyces cerevisiae

The role of NAD and NAD precursors on longevity and lifespan modulation in the budding yeast, Saccharomyces cerevisiae

Substrates of the MAPK Slt2: Shaping Yeast Cell Integrity

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