Substrates of the MAPK Slt2: Shaping Yeast Cell Integrity

González-Rubio, Gema; Sastre-Vergara, Lucía; Molina, Marı́a; Martı́n, Humberto; Fernández-Acero, Teresa

doi:10.3390/jof8040368

Cited by 20 publications

(17 citation statements)

References 155 publications

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“…MAPK pathway is essential for the formation and regulation of fungal cell walls [ 57 ]. Further analysis of DEGs showed that nine DEGs were mapped in the MAPK signaling pathway—yeast KEGG pathway, including six up-regulated DEGs ( fks2 , rsp5 , stt4 , bmh1,2 , ptp2,3 , mkk1,2 ) and three down-regulated DEGs ( yck1,2 , sln1 , ctt1 ).…”

Section: Resultsmentioning

confidence: 99%

Whole-Genome Sequencing and Transcriptome Analysis of Ganoderma lucidum Strain Yw-1-5 Provides New Insights into the Enhanced Effect of Tween80 on Exopolysaccharide Production

Cai

et al. 2022

JoF

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Ganoderma lucidum is an important medicinal mushroom widely cultured in Asian countries. Exopolysaccharides are bioactive compounds of G. lucidum with health benefits. Limited exopolysaccharide content hinders its extraction from G. lucidum. The addition of Tween80 had an enhanced effect on G. lucidum exopolysaccharide production in submerged fermentation. However, the mechanism of this effect remains unclear. In this study, we report on a high-quality assembly of G. lucidum strain yw-1-5 to lay the foundation for further transcriptome analysis. The genome sequence was 58.16 Mb and consisted of 58 scaffolds with an N50 of 4.78 Mb. A total of 13,957 protein-coding genes were annotated and Hi-C data mapped to 12 pseudo-chromosomes. Genes encoding glycosyltransferases and glycoside hydrolases were also obtained. Furthermore, RNA-seq was performed in a Tween80-treated group and control group for revealing the enhanced effect of Tween80 on exopolysaccharide production. In total, 655 genes were identified as differentially expressed, including 341 up-regulated and 314 down-regulated. Further analysis of differentially expressed genes showed that groups of MAPK, amino sugar and nucleotide sugar metabolism, autophagy, ubiquitin-mediated proteolysis, peroxisome, starch and sucrose metabolism, TCA cycle, glycolysis/gluconeogenesis KEGG pathway, glycosyltransferases and glycoside hydrolases played important roles in the enhanced effect of Tween80 on exopolysaccharide production. This work provides a valuable resource for facilitating our understanding of the synthesis of polysaccharides and accelerating the breeding of new strains with a high content of exopolysaccharides.

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Section: Resultsmentioning

confidence: 99%

Whole-Genome Sequencing and Transcriptome Analysis of Ganoderma lucidum Strain Yw-1-5 Provides New Insights into the Enhanced Effect of Tween80 on Exopolysaccharide Production

Cai

et al. 2022

JoF

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“…Mpk1 belongs to the cell wall integrity signalling pathway, which is responsible for the maintenance and biosynthesis of the cell wall and has been linked to various other cellular processes such as cell division, autophagy, proteasome assembly, and plasma membrane homeostasis ( 32 , 33 ). As Mpk1 contributes to stress-induced Shp1 phosphorylation, Shp1 could play a role downstream of this kinase.…”

Section: Discussionmentioning

confidence: 99%

Multiple phosphorylation of the Cdc48/p97 cofactor protein Shp1/p47 occurs upon cell stress in budding yeast

et al. 2023

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The homohexameric p97 complex, composed of Cdc48 subunits in yeast, is a crucial component of protein quality control pathways including ER-associated degradation. The complex acts to segregate protein complexes in an ATP-dependent manner, requiring the engagement of cofactor proteins that determine substrate specificity. The function of different Cdc48 cofactors and how they are regulated remains relatively poorly understood. In this study, we assess the phosphorylation of Cdc48 adaptor proteins, revealing a unique and distinctive phosphorylation pattern of Shp1/p47 that changed in response to TORC1 inhibition. Site-directed mutagenesis confirmed that this pattern corresponded to phosphorylation at residues S108 and S315 of Shp1, with the double-phosphorylated form becoming predominant upon TORC1 inhibition, ER-stress, and oxidative stress. Finally, we assessed candidate kinases and phosphatases responsible for Shp1 phosphorylation and identified two regulators. We found that cells lacking the kinase Mpk1/Slt2 show reduced Shp1 phosphorylation, whereas impaired PP1 phosphatase catalytic subunit (Glc7) activity resulted in increased Shp1 phosphorylation. Overall, these findings identify a phosphoregulation of Shp1 at multiple sites by Mpk1 kinase and PP1 phosphatase upon various stresses.

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“…In oomycetes and fungi, MAPK cascades also play a key role in the development and plant infection (Jiang et al, 2017;Gonzalez-Rubio et al, 2019). Sporangium formation is severely diminished in PsMPK1-silenced mutants (Li et al, 2014).…”

Section: Pathways Involved In Sporangium Formation and Cleavagementioning

confidence: 99%

Signal and regulatory mechanisms involved in spore development of Phytophthora and Peronophythora

Situ

Lin

et al. 2022

Front. Microbiol.

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Oomycetes cause hundreds of destructive plant diseases, threatening agricultural production and food security. These fungus-like eukaryotes show multiple sporulation pattern including the production of sporangium, zoospore, chlamydospore and oospore, which are critical for their survival, dispersal and infection on hosts. Recently, genomic and genetic technologies have greatly promoted the study of molecular mechanism of sporulation in the genus Phytophthora and Peronophythora. In this paper, we characterize the types of asexual and sexual spores and review latest progress of these two genera. We summarize the genes encoding G protein, mitogen-activated protein kinase (MAPK) cascade, transcription factors, RNA-binding protein, autophagy-related proteins and so on, which function in the processes of sporangium production and cleavage, zoospore behaviors and oospore formation. Meanwhile, various molecular, chemical and electrical stimuli in zoospore behaviors are also discussed. Finally, with the molecular mechanism of sporulation in Phytophthora and Peronophythora is gradually being revealed, we propose some thoughts for the further research and provide the alternative strategy for plant protection against phytopathogenic oomycetes.

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Substrates of the MAPK Slt2: Shaping Yeast Cell Integrity

Cited by 20 publications

References 155 publications

Whole-Genome Sequencing and Transcriptome Analysis of Ganoderma lucidum Strain Yw-1-5 Provides New Insights into the Enhanced Effect of Tween80 on Exopolysaccharide Production

Whole-Genome Sequencing and Transcriptome Analysis of Ganoderma lucidum Strain Yw-1-5 Provides New Insights into the Enhanced Effect of Tween80 on Exopolysaccharide Production

Multiple phosphorylation of the Cdc48/p97 cofactor protein Shp1/p47 occurs upon cell stress in budding yeast

Signal and regulatory mechanisms involved in spore development of Phytophthora and Peronophythora

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