Gene network of oil accumulation reveals expression profiles in developing embryos and fatty acid composition in Upland cotton

Zhao, Yanpeng; Wang, Yumei; Huang, Yi; Cui, Yupeng; Hua, Jinping

doi:10.1016/j.jplph.2018.06.002

Cited by 47 publications

(56 citation statements)

References 67 publications

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“…In the associated interval (block 3 ≈ 1.08 Mb), there were 69 and 70 annotated genes in the Gh reference genome TM-1 and Gb reference genome 3-79, respectively. A previously study showed that cottonseed oil accumulates rapidly at the middle-late stages (20 to 30 days post anthesis) [44]. Hence, we focused on the genes that are expressed in gradients in ovules with significantly higher expression levels than other tissues (root, stem, leaf and fiber) [10].…”

Section: Qtl Mapping For Soc and Substitution Mapping Of Qtl Locus Q-mentioning

confidence: 99%

Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton

Zhu

Wang

et al. 2020

BMC Genomics

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Background: The low genetic diversity of Upland cotton limits the potential for genetic improvement. Making full use of the genetic resources of Sea-island cotton will facilitate genetic improvement of widely cultivated Upland cotton varieties. The chromosome segments substitution lines (CSSLs) provide an ideal strategy for mapping quantitative trait loci (QTL) in interspecific hybridization. Results: In this study, a CSSL population was developed by PCR-based markers assisted selection (MAS), derived from the crossing and backcrossing of Gossypium hirsutum (Gh) and G. barbadense (Gb), firstly. Then, by whole genome re-sequencing, 11,653,661 high-quality single nucleotide polymorphisms (SNPs) were identified which ultimately constructed 1211 recombination chromosome introgression segments from Gb. The sequencing-based physical map provided more accurate introgressions than the PCR-based markers. By exploiting CSSLs with mutant morphological traits, the genes responding for leaf shape and fuzz-less mutation in the Gb were identified. Based on a high-resolution recombination bin map to uncover genetic loci determining the phenotypic variance between Gh and Gb, 64 QTLs were identified for 14 agronomic traits with an interval length of 158 kb to 27 Mb. Surprisingly, multiple alleles of Gb showed extremely high value in enhancing cottonseed oil content (SOC). Conclusions: This study provides guidance for studying interspecific inheritance, especially breeding researchers, for future studies using the traditional PCR-based molecular markers and high-throughput re-sequencing technology in the study of CSSLs. Available resources include candidate position for controlling cotton quality and quantitative traits, and excellent breeding materials. Collectively, our results provide insights into the genetic effects of Gb alleles on the Gh, and provide guidance for the utilization of Gb alleles in interspecific breeding.

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Section: Qtl Mapping For Soc and Substitution Mapping Of Qtl Locus Q-mentioning

confidence: 99%

Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton

Zhu

Wang

et al. 2020

BMC Genomics

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“…Fatty acid composition and its content determine the value and utilization of vegetable oil. The conventional cottonseed oil mainly consists of 26% palmitic acid (16:0), 58% linoleic acid (18:2 Δ9,12 ), 13% oleic acid (18:1Δ 9 ) and 2% stearic acid (18:0) (Zhao et al, 2018). Since linoleic acid is oxidatively unstable, cottonseed oil is easily oxidized and rancid, and thus it normally needs partial hydrogenation before food applications.…”

Section: Introductionmentioning

confidence: 99%

Stearoyl-ACP Δ9 Desaturase 6 and 8 (GhA-SAD6 and GhD-SAD8) Are Responsible for Biosynthesis of Palmitoleic Acid Specifically in Developing Endosperm of Upland Cotton Seeds

et al. 2019

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Palmitoleic acid (16:1Δ 9 ) is one kind of ω-7 fatty acids (ω-7 FAs) widely used in food, nutraceutical and industry. However, such high-valued ω-7 FA only has a trace level in mature seeds of cotton and other common oil crops. We found that palmitoleic acid (>10.58 Mol%) was specially enriched in developing cotton endosperm which is disappeared in its mature seed. The present study was conducted to investigate the mechanism underlying high accumulation of palmitoleic acid in developing endosperm but not in embryo of upland cotton ( Gossypium hirsutum L.) seed. Of 17 stearoyl-ACP Δ 9 desaturases (SAD) gene family members identified in upland cotton, six GhSADs may specifically work in the desaturation of palmitic acid (16:0-ACP) to produce palmitoleic acid (16:1Δ 9 -ACP), which were revealed by examining the key amino acids in the catalytic center and their cis -elements. Gene expression analysis showed that spatial patterns of these GhSADs were different in developing ovules, with GhA-SAD6 and GhD-SAD8 preferentially expressed in developing endosperms. Functional analysis by transient expression in Nicotiana benthamiana leaves and genetic complementary assay using yeast mutant BY4389 strain unable to synthesize unsaturated fatty acids demonstrated that GhA-SAD6 and GhD-SAD8 have strong substrate specificity for 16:0-ACP. In contrast, GhA-SAD5 and GhA-SAD7 exhibited high specific activity on 18:0-ACP. Taken together, these data evidence that GhA-SAD6 and GhD-SAD8 are responsible for making palmitoleic acid in developing cotton endosperms, and provide endogenous gene targets for genetic modification to enrich ω-7 FAs in cotton seed oil required for sustainable production of functionality-valued products.

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“…Further, Zhao et al. 13 displayed photographs of whole cottonseeds and it was evident that the seed coat had changed from white in color at 40 DPA to a distinct dark color by 45 DPA, and this coincides with the changes in starch reported by Hendrix 15 and the information about water content reported by Liu et al. 14 For protein, King and Leffler 16 showed that cottonseed derived water soluble proteins increased steadily during the final stages of seed maturation, while alkali soluble storage proteins increased rapidly from approximately 21 DPA and had stabilized by around 50 DPA.…”

Section: Discussionmentioning

confidence: 97%

“…For developing seeds, Zhao et al. 13 reported that seed oil content increased rapidly between 20 and 30 days post anthesis (DPA) and then remained constant for the rest of boll development. This suggests oil levels are not applicable to this boll cutting study, because the bolls of interest are older than 30 DPA.…”

Section: Discussionmentioning

confidence: 99%

Measuring the maturity of unopened cotton bolls with near infrared spectroscopy

Long

Bange

2020

Journal of Near Infrared Spectroscopy

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The boll cutting technique allows cotton producers to gauge the maturity of cotton bolls to help in their decision-making in the final stages of management of their crop leading up to harvest. The aim of this work was to assess the feasibility of using portable near infrared technology as a non-destructive rapid replacement for the manual boll cutting technique. Near infrared spectra (702–1100 nm) were collected from bolls using a Felix F-750 Produce Quality Meter. Spectra were successfully calibrated with two levels of boll maturity (immature and mature) using a partial least squares model for a population of 201 bolls. Independent validation of the model using separate boll populations predicted boll maturity correctly with an average accuracy of 96%. Calibrations that used spectra collected from bolls under field conditions performed similarly to calibrations that used spectra collected from the same bolls under controlled laboratory conditions. There was no preferred spectral sampling location on the bolls that resulted in better calibration performance. Calibration performance did not improve when spectral mathematical pre-treatments were used.

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Gene network of oil accumulation reveals expression profiles in developing embryos and fatty acid composition in Upland cotton

Cited by 47 publications

References 67 publications

Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton

Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton

Stearoyl-ACP Δ9 Desaturase 6 and 8 (GhA-SAD6 and GhD-SAD8) Are Responsible for Biosynthesis of Palmitoleic Acid Specifically in Developing Endosperm of Upland Cotton Seeds

Measuring the maturity of unopened cotton bolls with near infrared spectroscopy

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