Gene Functional Analysis Using Protoplast Transient Assays

Jung, Ha-il; Yan, Jiapei; Zhai, Zhiyang; Vatamaniuk, Olena K.

doi:10.1007/978-1-4939-2444-8_22

Cited by 26 publications

(23 citation statements)

References 29 publications

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“…Seeds of the model grass species B. distachyon (Bd21‐3) were obtained from the French National Institute for Agricultural Research (INRA). Seeds were grown hydroponically following the protocol of Jung, Yan, Zhai, and Vatamaniuk (2015). Briefly, seeds were soaked in water for 2 hr to soften the lamella and palea which were then removed using forceps.…”

Section: Methodsmentioning

confidence: 99%

“…The hydroponics set up consisted of two nested disposable cups that contained a foam disc cut to size with three slots cut to hold plants (see Figure S1). Each cup was filled with approximately 330 ml of full strength nutrient solution as per Jung et al (2015) except that Fe(III) EDTA concentration was doubled due to iron deficiencies detected in trial plants.…”

Section: Methodsmentioning

confidence: 99%

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Elevated atmospheric CO₂ suppresses jasmonate and silicon‐based defences without affecting herbivores

et al. 2020

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1. Elevated atmospheric CO 2 (eCO 2 ) not only increases plant growth but can also interfere with defence against insect herbivory through the disruption of the jasmonic acid (JA) pathway. Silicon (Si) plays an important role in plant stress tolerance and resistance to herbivory, particularly in grasses, many of which accumulate high amounts of Si. Activation of the JA pathway has been reported to stimulate Si uptake, while Si supplementation can alter both constitutive and induced phytohormone levels. A reduction in JA concentration under eCO 2 has the potential to reduce Si uptake in plants. Using both Si supplemented (Si+) and control (Si−) plants (Brachypodium distachyon)grown under ambient (400 ppm) and elevated (640 ppm) CO 2 concentrations, we tested how plant growth, foliar Si concentration and endogenous JA responded to methyl jasmonate (MeJA) application and the subsequent effects on insect herbivore performance (Helicoverpa armigera). Elevated CO2 reduced Si concentration by 19% and endogenous JA by 70% on average. MeJA significantly increased Si concentration in Si+ plants. Si+ plants had higher baseline JA levels compared to Si− plants under control conditions (i.e. no stress), however, when plants were chemically induced with MeJA, the JA response was on average 84% lower in Si+ plants compared to Si− plants. Plants without MeJA treatment showed the opposite response, that is, Si+ plants had higher baseline JA levels compared to Si− plants. Si significantly reduced herbivore consumption and growth rate. Despite eCO 2 significantly reducing both Si and endogenous JA, no effect was seen on herbivores feeding on eCO 2 plants. 4. Collectively our results suggest that Si alters the JA response of plants. We show that JA induces Si uptake, however, Si then reduces the JA response of plants under induced stress conditions. However, predicted increases in CO 2 levels within this century may significantly reduce Si-based mechanical defences against herbivory via a reduction of endogenous JA. K E Y W O R D S elevated CO 2 , herbivory, jasmonic acid, plant defence, silica S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Hall CR, Mikhael M, Hartley SE, Johnson SN. Elevated atmospheric CO 2 suppresses jasmonate and silicon-based defences without affecting herbivores. Funct

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Elevated atmospheric CO₂ suppresses jasmonate and silicon‐based defences without affecting herbivores

et al. 2020

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“…Protoplasts were isolated from 2‐week‐old seedlings grown on 1/2 MS plates as described previously (Jung, Yan, Zhai, & Vatamaniuk, ). To create fusion proteins with the EGFP tag, full‐length cDNA was PCR‐amplified and cloned into the pSAT6‐EGFP vector (Tzfira et al, ).…”

Section: Methodsmentioning

confidence: 99%

Nuclear pore complex components have temperature‐influenced roles in plant growth and immunity

Zhang

Wang

Kim

et al. 2020

Plant Cell & Environment

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Nuclear pore complexes (NPCs) are main channels controlling nucleocytoplasmic transport and are composed of approximately 30 nucleoporins (NUPs). Emerging evidence suggests that some NUP genes have specialized functions that challenge the traditional view of NPCs as structures of uniform composition. Here, we analysed the role of six outer-ring components of NPC at normal and warm growth temperatures by examining their loss-of-function mutants in Arabidopsis thaliana. All six NUP subunits, NUP85, NUP96, NUP 133, NUP 160, SEH1 and HOS1, have a non-redundant temperature-influenced function in one or more of the processes, including rosette growth, leaf architecture and intracellular immune receptor-mediated disease resistance. At the molecular level, NUP85 and NUP133 are required for mRNA export only at warm temperature and play a larger role in the localization of transcription factor at warm temperature. In addition, NUP96 and HOS1 are essential for the expression of high temperatureresponsive genes, which is correlated with their larger activity in facilitating nuclear accumulation of the transcription factor PIF4 at warm temperature. Our results show that subunits of NPC have differential roles at different temperatures, suggesting the existence of temperature-influenced NPC complexes and activities.

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“…A step-by-step protocol for protoplast isolation from leaf mesophyll cells of the Bd21-3 inbred line has been published by Jung et al (2015). We present the optimized protocol for the isolation of protoplasts from B. distachyon Bd21.…”

Section: Notementioning

confidence: 99%

A CRISPR/Cas9-Based Mutagenesis Protocol for Brachypodium distachyon and Its Allopolyploid Relative, Brachypodium hybridum

et al. 2020

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Gene Functional Analysis Using Protoplast Transient Assays

Cited by 26 publications

References 29 publications

Elevated atmospheric CO₂ suppresses jasmonate and silicon‐based defences without affecting herbivores

Elevated atmospheric CO₂ suppresses jasmonate and silicon‐based defences without affecting herbivores

Nuclear pore complex components have temperature‐influenced roles in plant growth and immunity

A CRISPR/Cas9-Based Mutagenesis Protocol for Brachypodium distachyon and Its Allopolyploid Relative, Brachypodium hybridum

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Gene Functional Analysis Using Protoplast Transient Assays

Cited by 26 publications

References 29 publications

Elevated atmospheric CO2 suppresses jasmonate and silicon‐based defences without affecting herbivores

Elevated atmospheric CO2 suppresses jasmonate and silicon‐based defences without affecting herbivores

Nuclear pore complex components have temperature‐influenced roles in plant growth and immunity

A CRISPR/Cas9-Based Mutagenesis Protocol for Brachypodium distachyon and Its Allopolyploid Relative, Brachypodium hybridum

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Product

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Elevated atmospheric CO₂ suppresses jasmonate and silicon‐based defences without affecting herbivores

Elevated atmospheric CO₂ suppresses jasmonate and silicon‐based defences without affecting herbivores