Gene expressions and biological activities of two novel antimicrobial peptides (AMPs) isolated from leucocytes of the rock bream, Oplegnathus fasciatus

Bae, Jin-Sol; Jeong, Ji-Min; Nam, Bo‐Hye; Kim, Juwon; Park, Jung Youn; Park, Chan-Il

doi:10.1016/j.aquaculture.2018.05.021

Cited by 6 publications

(6 citation statements)

References 57 publications

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“…On the contrary, E. tarda is resistant to all piscidins. A similar outcome is observed in other studies, with piscidins presenting a weak activity against E. tarda [25,54]. As for P. damselae subsp.…”

Section: Discussionsupporting

confidence: 90%

“…Piscidin genes and peptides are usually detected not only in sites with potential for pathogen entry, but also in immune related tissues of fish, including the intestine, spleen, head kidney, gills and skin. They can also be detected more broadly in organs such as the liver, heart or brain, depending on the piscidin and fish species [ 8 , 16 , 25 , 27 , 42 ]. Sea bass piscidin genes were detected in the organs above mentioned, being the gut the tissue with the highest basal expression levels.…”

Section: Discussionmentioning

confidence: 99%

“…These genes are constitutively expressed in tissues including the gills, skin, intestine, head kidney or spleen [ 8 , 9 , 16 , 22 , 23 ]. Several reports have shown a modulation of piscidin gene expression after infection with different pathogens [ 10 , 24 , 25 ]. Furthermore, piscidin peptides can be found in mucosal tissues at such concentrations that are lethal for pathogens [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

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The Diverse Piscidin Repertoire of the European Sea Bass (Dicentrarchus labrax): Molecular Characterization and Antimicrobial Activities

Barroso

Carvalho

et al. 2020

IJMS

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Fish rely on their innate immune responses to cope with the challenging aquatic environment, with antimicrobial peptides (AMPs) being one of the first line of defenses. Piscidins are a group of fish specific AMPs isolated in several species. However, in the European sea bass (Dicentrarchus labrax), the piscidin family remains poorly understood. We identified six different piscidins in sea bass, performed an in-depth molecular characterization and evaluated their antimicrobial activities against several bacterial and parasitic pathogens. Sea bass piscidins present variable amino acid sequences and antimicrobial activities, and can be divided in different sub groups: group 1, formed by piscidins 1 and 4; group 2, constituted by piscidins 2 and 5, and group 3, formed by piscidins 6 and 7. Additionally, we demonstrate that piscidins 1 to 5 possess a broad effect on multiple microorganisms, including mammalian parasites, while piscidins 6 and 7 have poor antibacterial and antiparasitic activities. These results raise questions on the functions of these peptides, particularly piscidins 6 and 7. Considering their limited antimicrobial activity, these piscidins might have other functional roles, but further studies are necessary to better understand what roles might those be.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Diverse Piscidin Repertoire of the European Sea Bass (Dicentrarchus labrax): Molecular Characterization and Antimicrobial Activities

Barroso

Carvalho

et al. 2020

IJMS

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“…Haemolysis has previously been used to determine the cytotoxic properties of AMPs, though methods used differ between research groups. Types of blood used for the assays can vary tremendously, with blood derived from species ranging from fish (rock bream) to human (Bae et al 2018, Rončević et al 2019. As a result of the membrane composition affecting the perceived haemolysis of the peptide, the modifications to the structure of the peptides could produce misleading haemolysis results when determining suitability for human application.…”

Section: Haemolysis As a Measure Of Cytotoxicitymentioning

confidence: 99%

“…The modification of the AMP C18G where leucine residues were substituted with phenylalanine residues improved the percentage viability of the HEK-293 cell line (Saint Jean et al 2018). As with many studies investigating modified AMPs (Oddo et al 2015, Bae et al 2018, Rončević et al 2019, the cytotoxicity screen was limited to one cell line which creates difficulties when observing cytotoxicity patterns. For Smp24, the incorporation of phenylalanine residues reduced cytotoxicity against the HepG2 and the HaCaT cell lines but the effect on HEK-293 cells was more varied, with only the K7F modification demonstrating a reduction in cytotoxicity.…”

Section: Cytotoxicity Of Smp24mentioning

confidence: 99%