Gene expression profiling of intestinal regeneration in the sea cucumber

Ortíz-Pineda, Pablo A.; Ramírez-Gómez, Francisco; Ortiz, Judit M. Pérez; González-Díaz, Sebastián; Jesús, Francisco Santiago-De; Hernández‐Pasos, Josué; Valle-Avila, Cristina Del; Rojas‐Cartagena, Carmencita; Suárez-Castillo, Edna C.; Tossas, Karen; Méndez-Merced, Ana T; Roig-López, José L.; Ortíz-Zuazaga, Humberto; García‐Arrarás, José E.

doi:10.1186/1471-2164-10-262

Cited by 101 publications

(124 citation statements)

References 88 publications

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“…The process of intestinal regeneration is complex and involves organogenesis, not merely wound healing. Therefore, many genes show differential expression during intestinal regeneration (Ortiz-Pineda et al, 2009). However, only a few genes playing important roles in intestinal regeneration in sea cucumbers have been thoroughly studied, such as Wnt9, Bmp1, ependymin, serum amyloid A, survivin, and mortalin (Santiago-Cardona et al, 2003;Suarez-Castillo et al, 2004;Zheng et al, 2006;Mashanov et al, 2010Mashanov et al, , 2012.…”

Section: Introductionmentioning

confidence: 99%

“…Intestinal regeneration of sea cucumbers employs morphallaxis (mainly cell migration) at the early stage and epimorphosis (mainly cell division) at the later stage (García-Arrarás et al, 1998). Accompanying the development of new technology, researchers have screened candidate genes that were associated with visceral regeneration by gene microarray or RNA sequencing to study the molecular mechanism of visceral regeneration (Rojas-Cartagena et al, 2007;Ortiz-Pineda et al, 2009;Sun et al, 2011). The process of intestinal regeneration is complex and involves organogenesis, not merely wound healing.…”

Section: Introductionmentioning

confidence: 99%

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Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus

Sun

Yang²,

Chen³

et al. 2013

Genet. Mol. Res.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus

Sun

Yang²,

Chen³

et al. 2013

Genet. Mol. Res.

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“…Although there are still very few examples of brittle stars showing extremely slow regeneration (1 in a temperate region, 2 in the Antarctic), there is no obvious ecological or physiological factor linking all 3 that could potentially hold the key to understanding why the rate should be so slow in these contrasting species or why the initial regeneration phases are so pronounced in Antarctic species. Whilst histological analyses can identify cell types involved in the regeneration processes (Biressi et al 2010), molecular analyses can potentially offer more fine-scale insights, not just into particular biochemical and signalling pathways (Bannister et al 2005, Burns et al 2011), but they may also generate a more general overview with large-scale Expressed Sequence Tag (EST) projects (Ortiz-Pineda et al 2009) and the application of next-generation sequencing technologies. Indeed, such technologies may be essential to identifying why regeneration is so slow in these species.…”

Section: Discussionmentioning

confidence: 99%

Slow arm regeneration in the Antarctic brittle star Ophiura crassa (Echinodermata, Ophiuroidea)

Clark¹,

Souster²

2012

Aquat. Biol.

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Regeneration of arms in brittle stars is thought to proceed slowly in low temperature environments. Here a survey of natural arm damage and arm regeneration rates is documented in the Antarctic brittle star Ophiura crassa. This relatively small ophiuroid, a detritivore found amongst red macroalgae, displays high levels of natural arm damage and repair. This is largely thought to be due to ice damage in the shallow waters it inhabits. The time scale of arm regeneration was measured in an aquarium-based 10 mo experiment. There was a delayed regeneration phase of 7 mo before arm growth was detectable in this species. This is 2 mo longer than the longest time previously described, which was in another Antarctic ophiuroid, Ophionotus victoriae. The subsequent regeneration of arms in O. crassa occurred at a rate of approximately 0.16 mm mo −1 . To date, this is the slowest regeneration rate known of any ophiuroid. The confirmation that such a long delay before arm regeneration occurs in a second Antarctic species provides strong evidence that this phenomenon is yet another characteristic feature of Southern Ocean species, along with deferred maturity, slowed growth and development rates. It is unclear whether delayed initial regeneration phases are adaptations to, or limitations of, low temperature environments.KEY WORDS: Ophiura crassa · Ophionotus victoriae · Autoecology · Ophioderma longicaudum · Brooder · Q 10 coefficientResale or republication not permitted without written consent of the publisher

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“…In an attempt to identify novel biomarkers for acute mesenteric ischemia in a porcine model, Block et al [2] implemented microarray analysis of intestinal tissue samples. Additionally, Ortiz-Pineda et al [3] recently published detailed results on gene expression profiling of intestinal regeneration in echinoderms.…”

Section: Introductionmentioning

confidence: 99%

Searching for the Molecular Benchmark of Physiological Intestinal Anastomotic Healing in Rats: An Experimental Study

Seifert¹,

Seifert²,

Kulemann³

et al. 2014

Eur Surg Res

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Purpose: This investigation focuses on the physiological characteristics of gene transcription of intestinal tissue following anastomosis formation. Methods: In eight rats, end-to-end ileo-ileal anastomoses were performed (n = 2/group). The healthy intestinal tissue resected for this operation was used as a control. On days 0, 2, 4 and 8, 10-mm perianastomotic segments were resected. Control and perianastomotic segments were examined with an Affymetrix microarray chip to assess changes in gene regulation. Microarray findings were validated using real-time PCR for selected genes. In addition to screening global gene expression, we identified genes intensely regulated during healing and also subjected our data sets to an overrepresentation analysis using the Gene Ontology (GO) and Kyoto Encyclopedia for Genes and Genomes (KEGG). Results: Compared to the control group, we observed that the number of differentially regulated genes peaked on day 2 with a total of 2,238 genes, decreasing by day 4 to 1,687 genes and to 1,407 genes by day 8. PCR validation for matrix metalloproteinases-3 and -13 showed not only identical transcription patterns but also analogous regulation intensity. When setting the cutoff of upregulation at 10-fold to identify genes likely to be relevant, the total gene count was significantly lower with 55, 45 and 37 genes on days 2, 4 and 8, respectively. A total of 947 GO subcategories were significantly overrepresented during anastomotic healing. Furthermore, 23 overrepresented KEGG pathways were identified. Conclusion: This study is the first of its kind that focuses explicitly on gene transcription during intestinal anastomotic healing under standardized conditions. Our work sets a foundation for further studies toward a more profound understanding of the physiology of anastomotic healing.

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Gene expression profiling of intestinal regeneration in the sea cucumber

Cited by 101 publications

References 88 publications

Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus

Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus

Slow arm regeneration in the Antarctic brittle star Ophiura crassa (Echinodermata, Ophiuroidea)

Searching for the Molecular Benchmark of Physiological Intestinal Anastomotic Healing in Rats: An Experimental Study

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