Gene expression profiles revealing the mechanisms of anti-androgen- and estrogen-induced feminization in fish

Filby, Amy L.; Thorpe, Karen L.; Maack, Gerd; Tyler, Charles R.

doi:10.1016/j.aquatox.2006.12.003

Cited by 279 publications

(190 citation statements)

References 62 publications

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“…Environmental Protection Agency, 2007;Organisation for Economic Cooperation and Development, 2009b). While some endocrine mechanisms are reliably detected in these assays, anti-androgens are more difficult to identify in an unambiguous fashion (Seki et al, 2006;Katsiadaki et al, 2006;Filby et al, 2007;U.S. Environmental Protection Agency, 2007;Organisation for Economic Cooperation and Development, 2009b).…”

Section: Discussionmentioning

confidence: 99%

“…Chemicals that inhibit steroidogenesis consistently decrease VTG levels in female fish. However, the suite of responses produced by anti-androgenic chemicals in sexually mature fish can be somewhat ambiguous, making it difficult to clearly diagnose this important pathway Katsiadaki et al, 2006;Seki et al, 2006;Filby et al, 2007;Organisation for Economic Cooperation and Development, 2009b). A wide variety of known or potential environmental contaminants, including some dicarboximide fungicides (e.g., vinclozolin), conazole fungicides (e.g., prochloraz), organochlorine insecticides (e.g., p,p -DDE), urea-based herbicides (e.g., linuron), polybrominated diphenyl ethers, and pharmaceuticals (e.g., flutamide) have been shown to bind to the AR, and cause anti-androgenic effects in vivo (Gray et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Use of chemical mixtures to differentiate mechanisms of endocrine action in a small fish model

et al. 2010

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a b s t r a c tVarious assays with adult fish have been developed to identify potential endocrine-disrupting chemicals (EDCs) which may cause toxicity via alterations in the hypothalamic-pituitary-gonadal (HPG) axis. These assays can be sensitive and highly diagnostic for key mechanisms such as agonism of the estrogen and androgen receptors (ERs, ARs) and inhibition of steroid synthesis. However, most of the tests do not unambiguously identify AR antagonists. The purpose of this work was to explore the utility of a mixture test design with the fathead minnow (Pimephales promelas) for detecting different classes of EDCs including AR antagonists. Adults of both sexes were exposed via the water to EDCs with diverse mechanisms of action in the absence or presence of 17␤-trenbolone (TB), a potent AR agonist which masculinizes female fathead minnows. Similar to previous studies with the model AR antagonists flutamide and vinclozolin, exposure of females to the AR antagonist cyproterone acetate in the presence of TB decreased expression of an easily-observed masculinization response, nuptial tubercle formation. Mixture studies with TB and the model ER agonists, 17␣-ethinylestradiol and bisphenol A, also showed inhibition of tubercle formation in the females, but unlike the AR antagonists, the estrogens markedly induced synthesis of vitellogenin (VTG: egg yolk protein), particularly in males. The ER agonists also offset TB-induced depressions in plasma VTG concentrations in female fish. Additional mixture experiments were conducted with TB and triclocarban, an anti-microbial reported to enhance AR-mediated responses, or ammonia, a "negative control" with no known direct effects on HPG function. Neither chemical affected VTG status in males or females in the absence or presence of TB; however, both slightly enhanced TB-induced tubercle formation in females. Based on studies described herein and elsewhere with the fathead minnow, a TB co-exposure assay appears to be an effective approach for clearly identifying AR antagonists as well as potential EDCs with other relevant mechanisms of action.Published by Elsevier B.V.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of chemical mixtures to differentiate mechanisms of endocrine action in a small fish model

et al. 2010

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“…previous study, exposure of fish to the estrogenic EE2 or antiandrogenic flutamide resulted in down-regulation of AR mRNA expression (Filby et al, 2007). However, it is still unclear whether TBBPA exhibits an anti-androgenic activity due to its down-regulation of ARα mRNA expression in the testis of mosquitofish.…”

Section: Expression Patterns Of Hormonal-response Genes In Mosquitofimentioning

confidence: 93%

Hormonal effects of tetrabromobisphenol A using a combination of in vitro and in vivo assays

Huang

Ying

Liang

et al. 2013

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…The molecular foundation for the feminisation effect of androgens and oestrogens in fish is not well understood. Filby et al (2007) studied the expression of 22 genes (linked to processes controlled by androgens and oestrogens, i.e. reproduction, growth, maturation) localised in the livers and gonads of female and male fathead minnows.…”

Section: Discussionmentioning

confidence: 99%

Impact of butyl benzyl phthalate on development of the reproductive system of European pikeperch, Sander lucioperca (L.)

Jarmołowicz

Demska‐Zakęś

Zakęś

2014

Acta Veterinaria Hungarica

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The effect of butyl benzyl phthalate (BBP) on the sex differentiation process of fish is practically unknown. The experimental material of this study was juvenile European pikeperch [Sander lucioperca (L.)], which is gonochoristic, undergoes immediate sex differentiation, and has a fixed gonad differentiation period. The fish were fed a diet supplemented with BBP (during the sex differentiation phase: age 61-96 days post hatch) in the following quantities: 1.0; 2.0; 4.0; 8.0; 16.0 g BBP kg -1 feed. The control feed was a xenobiotic-free base feed. In the present experiment lasting 10 weeks, the survival and growth of fish, the histopathological changes of the fish gonads and the sex ratio were evaluated. After administration of the two highest doses of BBP, growth inhibition of the fish was observed. BBP also seriously disturbed the gonadal differentiation process of pikeperch. All analysed concentrations of BBP delayed testicular development and, at concentrations of 4.0, 8.0 and 16.0 g BBP kg -1 , induction of the feminisation process was observed. The sex ratio was distinctly disrupted in groups receiving 8.0 and 16.0 g BBP kg -1 .

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Gene expression profiles revealing the mechanisms of anti-androgen- and estrogen-induced feminization in fish

Cited by 279 publications

References 62 publications

Use of chemical mixtures to differentiate mechanisms of endocrine action in a small fish model

Use of chemical mixtures to differentiate mechanisms of endocrine action in a small fish model

Hormonal effects of tetrabromobisphenol A using a combination of in vitro and in vivo assays

Impact of butyl benzyl phthalate on development of the reproductive system of European pikeperch, Sander lucioperca (L.)

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