Gene expression profiles of human neuroblastoma cells exposed to CuO nanoparticles and Cu ions

Jang, Seoyoung; Oh, Min-Su; Yang, Sung Ik; Cho, Eun-Min

doi:10.1007/s13206-016-0209-5

Cited by 12 publications

(10 citation statements)

References 25 publications

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“…GADD45A1 (Growth Arrest And DNA Damage Inducible Alpha 1), which is known to be highly expressed in response to presence of DNA-damaging factors as well as in stressful growth-arrest conditions and during the apoptosis [55]. It was previously reported that exposure to nanoparticles such as chitosan NPs [56] and CuO NPs [57] led to upregulation of GADD45A1 in cells. Here, the expression level was increased significantly only in case of PHT2%-based LLCNPs, indicating that this pathway is affected in case of these PHT-based samples at selected concentration range.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive and comparative studies on nanocytotoxicity of glyceryl monooleate- and phytantriol-based lipid liquid crystalline nanoparticles

et al. 2021

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Background Lipid liquid crystalline nanoparticles (LLCNPs) emerge as a suitable system for drug and contrast agent delivery. In this regard due to their unique properties, they offer a solubility of a variety of active pharmaceutics with different polarities increasing their stability and the possibility of controlled delivery. Nevertheless, the most crucial aspect underlying the application of LLCNPs for drug or contrast agent delivery is the unequivocal assessment of their biocompatibility, including cytotoxicity, genotoxicity, and related aspects. Although studies regarding the cytotoxicity of LLCNPs prepared from various lipids and surfactants were conducted, the actual mechanism and its impact on the cells (both cancer and normal) are not entirely comprehended. Therefore, in this study, LLCNPs colloidal formulations were prepared from two most popular structure-forming lipids, i.e., glyceryl monooleate (GMO) and phytantriol (PHT) with different lipid content of 2 and 20 w/w%, and the surfactant Pluronic F-127 using the top-down approach for further comparison of their properties. Prepared formulations were subjected to physicochemical characterization and followed with in-depth biological characterization, which included cyto- and genotoxicity towards cervical cancer cells (HeLa) and human fibroblast cells (MSU 1.1), the evaluation of cytoskeleton integrity, intracellular reactive oxygen species (ROS) generation upon treatment with prepared LLCNPs and finally the identification of internalization pathways. Results Results denote the higher cytotoxicity of PHT-based nanoparticles on both cell lines on monolayers as well as cellular spheroids, what is in accordance with evaluation of ROS activity level and cytoskeleton integrity. Detected level of ROS in cells upon the treatment with LLCNPs indicates their insignificant contribution to the cellular redox balance for most concentrations, however distinct for GMO- and PHT-based LLCNPs. The disintegration of cytoskeleton after administration of LLCNPs implies the relation between LLCNPs and F-actin filaments. Additionally, the expression of four genes involved in DNA damage and important metabolic processes was analyzed, indicating concentration–dependent differences between PHT- and GMO-based LLCNPs. Conclusions Overall, GMO-based LLCNPs emerge as potentially more viable candidates for drug delivery systems as their impact on cells is not as deleterious as PHT-based as well as they were efficiently internalized by cell monolayers and 3D spheroids. Graphic Abstract

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Section: Discussionmentioning

confidence: 99%

Comprehensive and comparative studies on nanocytotoxicity of glyceryl monooleate- and phytantriol-based lipid liquid crystalline nanoparticles

et al. 2021

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“…Three of these NPs (Co 3 O 4 , Sb 2 O 3 , CuO NPs) were additionally studied using a trans epithelial electrical resistance (TEER) assay, to evaluate the adverse effects of these NPs on the physiological membrane integrity of the epithelial cell layers of Caco2 and A549. Those three NPs were chosen for the TEER assay for different reasons: CuO NPs are well studied and known to be toxic at 10 lg/ mL (Ivask et al 2015;Jang et al 2016). 100 lg/mL of Co 3 O 4 or Sb 2 O 3 NPs were not very toxic after 24 h for epithelial cells Caco2 and A549, but 6 lg/mL was lethal for murine fibroblasts Balb/c 3T3 after 7 days.…”

Section: Introductionmentioning

confidence: 99%

Toxicity of antimony, copper, cobalt, manganese, titanium and zinc oxide nanoparticles for the alveolar and intestinal epithelial barrier cells in vitro

et al. 2016

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Heavy metals are found naturally on Earth and exposure to them in the living environment is increasing as a consequence of human activity. The toxicity of six different metal oxide nanoparticles (NP) at different points in time was compared using resazurin assay. After incubating Caco2 and A549 cells with 100 lg/mL of Sb 2 O 3 , Mn 3 O 4 and TiO 2 nanoparticles (NPs) for 24 h no toxic effects were observed while Co 3 O 4 and ZnO NPs had moderate effects and CuO NPs were toxic below 100 lg/mL (24 h EC 25 = 11 for A549 and 71 lg/mL for Caco2). The long-term monitoring (up to 9 days) of cells to NPs revealed that the toxic effects of Mn 3 O 4 and Sb 2 O 3 NPs remarkably increased over time. The 9 day EC 50 values for Sb 2 O 3 NPs were 22 and 48 lg/mL for A549 and Caco2 cells; and for Mn 3 O 4 NPs were 47 and 29 lg/mL for A549 and Caco2 cells, respectively. In general, the sensitivity of the cell lines in the resazurin assay was comparable. Trans epithelial electrical resistance (TEER) measurements were performed for both cell types exposed to Co 3 O 4 , Sb 2 O 3 and CuO NPs. In TEER assay, the Caco2 cells were more susceptible to the toxic effects of these NPs than A549 cells, where the most toxic NPs were the Sb 2 O 3 NPs: the permeability of the Caco2 cell layer exposed to 10 lg/mL Sb 2 O 3 NPs already increased after 24 h of exposure.Electronic supplementary material The online version of this article

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“…In vitro toxicological effects of CuO NPs have been demonstrated on different cell types: human lymphocytes and erythrocytes (Dey et al, 2019), airway epithelial (HEp‐2) cells (Fahmy & Cormier, 2009), human alveolar epithelial cells A549 (Ivask et al, 2015), human airway smooth muscle cells (Berntsen et al, 2010), mouse pulmonary microvascular endothelial cells (Yu et al, 2010), human neuroblastoma SH‐SY5Y cells (Jang, Oh, Yang, & Cho, 2016), human epithelial colorectal cells (Caco‐2) and murine fibroblast cell line Balb/c 3T3 (Ivask et al, 2015). According to these works, the main mechanism concerning the toxic response elicited by CuO NPs involves particle dissolution with a consequent release of toxic metal ions, activation of signaling cascades, induction of inflammation, ROS generation with induction of cellular oxidative stress, protein and cytoplasmic membrane damages.…”

Section: Discussionmentioning

confidence: 99%

In vitro copper oxide nanoparticle toxicity on intestinal barrier

Bertero

Colombo

Cortinovis

et al. 2020

J of Applied Toxicology

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The use of CuO nanoparticles (NPs) has increased greatly and their potential effects on human health need to be investigated. Differentiated Caco-2 cells were treated from the apical (Ap) and the basolateral (Bl) compartment with different concentrations (0, 10, 50 and 100 μg/mL) of commercial or sonochemically synthesized (sono) CuO NPs. Sono NPs were prepared in ethanol (CuOe) or in water (CuOw), obtaining CuO NPs differing in size and shape. The effects on the Caco-2 cell barrier were assessed via transepithelial electrical resistance (TEER) evaluation just before and after 1, 2 and 24 hours of exposure and through the analysis of cytokine release and biomarkers of oxidative damage to proteins after 24 hours. Sono CuOe and CuOw NPs induced a TEER decrease with a dose-dependent pattern after Bl exposure. Conversely, TEER values were not affected by the Ap exposure to commercial CuO NPs and, concerning the Bl exposure, only the lowest concentration tested (10 μg/mL) caused a TEER decrease after 24 hours of exposure. An increased release of interleukin-8 was induced by sono CuO NPs after the Ap exposure to 100 μg/mL and by sono and commercial CuO after the Bl exposure to all the concentrations. No effects of commercial and sono CuO NPs on interleukin-6 (with the only exception of 100 μg/mL Bl commercial CuO) and tumor necrosis factor-α release were observed. Ap treatment with commercial and CuOw NPs was able to induce significant alterations on specific biomarkers of protein oxidative damage (protein sulfhydryl group oxidation and protein carbonylation). K E Y W O R D S caco-2 cells, copper oxide, in vitro, nanoparticles 1 | INTRODUCTION The use of metal and metal oxide nanoparticles (NPs) has grown exponentially nowadays due to their potential applications as catalysts, sensors, components of batteries, magnetic storage media, solar cells and paints, just to mention some, covering a wide range of fields (e.g., electronics, medicine, food, agriculture, cosmetics) (Chavali & Nikolova, 2019; Madhav et al., 2017). In particular, copper oxide (CuO) NPs are becoming increasingly popular as they are preferred among other NPs because of their peculiar properties such as hardness, conductivity, formability and strength of alloys (Madhav et al., 2017). Cu is an essential trace element that plays many

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Gene expression profiles of human neuroblastoma cells exposed to CuO nanoparticles and Cu ions

Cited by 12 publications

References 25 publications

Comprehensive and comparative studies on nanocytotoxicity of glyceryl monooleate- and phytantriol-based lipid liquid crystalline nanoparticles

Comprehensive and comparative studies on nanocytotoxicity of glyceryl monooleate- and phytantriol-based lipid liquid crystalline nanoparticles

Toxicity of antimony, copper, cobalt, manganese, titanium and zinc oxide nanoparticles for the alveolar and intestinal epithelial barrier cells in vitro

In vitro copper oxide nanoparticle toxicity on intestinal barrier

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