Gene expression profiles in the intestine of lipopolysaccharide-challenged piglets

Abstract

Bowel diseases are common in human and animals and are characterized by intestinal dysfunction and injury. A well-established porcine model of intestinal injury can be induced by lipopolysaccharide (LPS), an endotoxin released from the cell wall of pathogenic bacteria. LPS affects the expression of genes associated with intestinal immune response, mucosal growth, energy metabolism, absorption, mucosal barrier function, and antiviral function. Transcriptional analysis of intestinal genes reveals that the duodenum, jejunum, ileum and colon respond to LPS challenge in a similar pattern. Moreover, the jejunum and ileum exhibit greater responses to LPS challenge than the duodenum and colon with regard to gene expression. Additionally, over 85% of genes are co-expressed along the small and large intestines and there is a clear distinction in gene expression patterns amongst the different intestinal segments in pigs. These findings have important implications for underlying molecular mechanisms responsible for endotoxin-induced intestinal injury and dysfunction.

Microarray analysis reveals the inhibition of intestinal expression of nutrient transporters in piglets infected with porcine epidemic diarrhea virus

“…RNA could be used for RT-PCR analysis and microarray assay when it had a 28S/18S rRNA ratio of >1.8 85 . Only those samples that had an OD 260 /OD 280 ratio of approximately 2.0 and showed no degradation (RNA integrity number of >7.0) were used to generate labeled targets 86 . RNA integrity number was determined using an Agilent Bioanalyzer 2100 (Agilent Technologies, Inc., Santa Clara, CA, USA).…”

“…The primer pairs used are shown in Supplementary File S1. The qPCR was performed using the SYBR Premix Ex TaqTM (Takara, Dalian, China) on an Applied Biosystems 7500 Fast Real-Time PCR System (Foster City, CA, USA) 86 . The total volume of PCR reaction system was 50 µL, which contained 0.2 µM of each primer, 25 µL of SYBR Premix Ex TaqTM (2x) and 4 µL of cDNA.…”

Quantitative Proteomic Analysis Reveals Antiviral and Anti-inflammatory Effects of Puerarin in Piglets Infected With Porcine Epidemic Diarrhea Virus

“…Then the viral gene expression was detected by using the droplet digital PCR (dd PCR) method as described previously (31). Primers used in the present study are listed in Table 1 (32)(33)(34)(35).…”

Current advances in renewal mechanisms of intestinal epithelial cells along the crypt-villus axis

“…(ataxia telangiectasia-mutated gene, ATM)、促炎因子白 细胞介素-8(interleukin-8, IL-8)和肿瘤坏死因子(tumor necrosis factor, TNF)表达上调, 而细胞周期调控相关 基因E2F转录因子5(E2F transcription factor 5, E2F5)和 SMAD家族成员4(SMAD family member 4, Smad4)表达 下调; 表明断奶引起免疫抑制, 使细胞周期停滞, 加速 凋亡 [25] . 在脂多糖(lipopolysaccharides, LPS)诱导的仔 猪肠道疾病模型实验中发现, 肠道免疫反应、黏膜生 长、能量代谢、吸收、黏膜屏障功能、抗病毒功能 等相关基因的表达均受到影响 [26] . 另外, 断奶还使仔 猪抗氧化能力显著下降; 肠道细胞内与氧化应激相关 基因表达增加, 自由基代谢异常, 血清中NO和H 2 O 2 含 量显著增加, 而体内自由基水平与肠道结构与功能有 密切关联 [27] .…”