Gene expression patterns of abalone, Haliotis tuberculata, during successive infections by the pathogen Vibrio harveyi

The early stages of the immune response of the European abalone Haliotis tuberculata to a Vibrio harveyi infection

“…A modulation of Glutamine metabolism via activation or inhibition of GLS synthesis or gene expression during infection process has been previously reported in humans and marine invertebrates such as the bivalvia Tegillarca granosa and Chlamys farreri (Karinch et al, 2001; Exposure time * * Unexposed Exposed Wilmore and Shabert, 1998). A down-regulation of GLS expression has also been observed in infected muscle of abalone after 6 days of exposure (Travers et al, 2010). This reduced general metabolism may be a consequence of vibriosis or a response of the European abalone to vibriosis.…”

“…Seven genes were chosen for gene expression analysis due to their implication in general metabolism -Glutamine Synthase (Gls), in cell movement -Actin, in immune response -the subunit H of Ferritin, Clathrin, Macrophage expressed gene (Mpeg), and in signal transduction -Rel/ nuclear factor-kappa B (Rel/NF-κB) and MAP kinase-interacting kinase (Mnk) genes, and their putative involvement during abalone infection (Jiang and Wu, 2007;Travers et al, 2009bTravers et al, , 2010Wang et al, 2008b). The gene encoding Elongation Factor 2 (Ef2) was selected as reference gene (Table 1) because of its stable expression in all samples and time points.…”

“…RT-qPCR measurements were performed as previously described (Travers et al, 2010). Each run included a negative control in which sterile water replaced cDNA sample, and a reference sample constituted by a pool of all the samples -including both control and exposed samples.…”

“…In shrimps, ferritin protects against V. harveyi infection (Maiti et al, 2010). In the European abalone, Ferritin was among the few genes upregulated in individuals that survived the infection by V. harveyi (Travers et al, 2010). Therefore, the late Actin and Ferritin overexpression could represent an alternative response of the abalone to V. harveyi infection to counter the impaired phagocytosis.…”

Effect of linear alkylbenzene mixtures and sanitary sewage in biochemical and molecular responses in pacific oyster Crassostrea gigas

“…Previous study indicated the potential of this gene for use as a biomarker of exposure to sanitary sewage-contaminated sites (Medeiros et al 2008a, b). FABPs are cytosolic proteins involved in the absorption, transport, and compartmentalization of fatty acids and can be stimulated under a variety of circumstances in which the lipids are mobilized to provide the required metabolic fuel (Travers et al 2010). Fatty acids act as a source of energy for metabolism and can act as signal regulators of enzymatic systems, modulating gene expression pathways, growth, survival, inflammatory, and metabolic responses (Furuhashi and Hotamisligil 2008).…”