Gene expression patterns associated with human placental trophoblast differentiation

Jiang, Shi‐Wen; Zhou, Wei; Wang, Jianhao; Little, Lauren; Leaphart, Lynn W.; Jay, Jacob White; Igbinigie, Eseosaserea; Chen, Haibin; Li, Jinping

doi:10.1016/j.cca.2018.01.012

Cited by 8 publications

(6 citation statements)

References 32 publications

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“…During differentiation of TSCs, Ccne2 and Cdk6 might facilitate the transition into S phase and assembly of pre-replication complexes on DNA for re-replication [ 46 , 47 ]. This finding is in agreement with recent gene expression profiling of human trophoblast differentiation using microarrays, where several cell cycle-related genes were downregulated in syncytiotrophoblasts [ 48 ] and supports the observation that TGCs do not undergo a conventional mitotic cell cycle. Some of the genes (e.g., Cdc6 , Cdc20 , and Ccnb2 ) were downregulated in both systems.…”

Section: Discussionsupporting

confidence: 92%

Transcriptomic analysis reveals differential gene expression, alternative splicing, and novel exons during mouse trophoblast stem cell differentiation

et al. 2020

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Background: Differentiation of mouse trophoblast stem cells (TSCs) to trophoblast giant cells (TGCs) has been widely used as a model system to study placental development and function. While several differentially expressed genes, including regulators of TSC differentiation, have been identified, a comprehensive analysis of the global expression of genes and splice variants in the two cell types has not been reported. Results: Here, we report~7800 differentially expressed genes in TGCs compared to TSCs which include regulators of the cell cycle, apoptosis, cytoskeleton, cell mobility, embryo implantation, metabolism, and various signaling pathways. We show that several mitotic proteins, including Aurora A kinase, were downregulated in TGCs and that the activity of Aurora A kinase is required for the maintenance of TSCs. We also identify hitherto undiscovered, celltype specific alternative splicing events in 31 genes in the two cell types. Finally, we also report 19 novel exons in 12 genes which are expressed in both TSCs and TGCs. Conclusions: Overall, our results uncover several potential regulators of TSC differentiation and TGC function, thereby providing a valuable resource for developmental and molecular biologists interested in the study of stem cell differentiation and embryonic development.

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Section: Discussionsupporting

confidence: 92%

Transcriptomic analysis reveals differential gene expression, alternative splicing, and novel exons during mouse trophoblast stem cell differentiation

et al. 2020

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“…The main limitation of this study is the sample size, and it is necessary to replicate this methodology in a larger sample size. Additionally, it has been shown that gene expression could vary with placental trophoblast differentiation 53 and with gestational age 54 . Although all deliveries were in the third trimester, differences in gestational age at the time of delivery between patients with EO‐PE and controls may have affected the results.…”

Section: Discussionmentioning

confidence: 99%

Expression of tissue factor and thrombomodulin in the placentas of pregnancies affected by early‐onset and late‐onset preeclampsia

Ayala-Ramírez

Gámez

Castro-Pontón

et al. 2021

J of Obstet and Gynaecol

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Aim To analyze the differential genetic expression and protein localization of thrombomodulin (THBD) and tissue factor (F3) in the placentas of pregnancies affected by preeclampsia. Methods We assessed the expression of THBD and F3 by immunohistochemistry and real‐time polymerase chain reaction (PCR) in placentas from 20 PE cases: 10 early‐onset PE placentas, 10 late‐onset PE placentas, and 10 control cases (normal pregnancies). Results In cases, we found higher THBD and F3 RNA levels in placental tissue. Protein expression in controls differed from that in late‐onset PE placentas, which had lower THBD levels in syncytiotrophoblasts and amniotic cells. Likewise, late‐onset PE placentas exhibited comparatively lower F3 expression in the perivillous fibrin. In contrast, early‐onset PE had high F3 expression in the subdecidual fibrin. We found no significant differences in the F3/THBD ratio between the groups. Conclusion Our study supports evidence that shows the involvement of F3 and THBD in placental disorders. Furthermore, this finding contributes to a better understanding of the physio‐pathological role that these molecules may play in the development of this heterogeneous disease.

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“…Placental trophoblast cell differentiation and invasion of extravillous cytotrophoblast cells to endometrium and myometrium and replacing with an endothelial layer of the maternal spiral arteries are necessary for the formation of the normal placenta and significant changes in gene expression pattern were detected during this stage (4,14,15). Dysregulation of these gene expression patterns and consequent any abnormality during placenta formation may cause preeclampsia that is the primary cause of maternal and perinatal morbidity and mortality worldwide (4,11).…”

Section: Discussionmentioning

confidence: 99%

Placental Maspin Gene Expression Pattern May Be Used to Distinguish Between Preeclampsia and Intrauterine Growth Restriction

Önalan

Terzi

Tohma

et al. 2021

Gynecol Obstet Reprod Med

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Objective: Maspin is one of the members of serin protease inhibitor superfamily, and acts as a tumor suppressor protein. Here we report maspin expression in placental tissues of preeclampsia or intrauterine growth retardation pregnancies.Study Design: Maspin expression level was analyzed by semi-quantitative RT-PCR method. For this purpose, placental tissue samples were obtained from pregnancies 13 with intrauterine growth retardation, and 6 with preeclampsia, and also 12 tissues from women without preeclampsia or intrauterine growth retardation history were included as controls. Results: Maspin expression was found to be 11-fold higher in placentas from preeclampsia patients than placentas from intrauterine growth retardation patients (p=0.036). However, there was no difference between preeclampsia and control groups (p=0.76), and intrauterine growth retardation and control groups (p=0.849).Conclusion: As far as we know this is the first study reporting expression level of maspin in placental tissues of pregnancies with intrauterine growth retardation. These findings support that molecular pathology between preeclampsia and IUGR might be different at the molecular level and maspin levels in the placenta may be used to differentiate preeclampsia from IUGR.

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Gene expression patterns associated with human placental trophoblast differentiation

Cited by 8 publications

References 32 publications

Transcriptomic analysis reveals differential gene expression, alternative splicing, and novel exons during mouse trophoblast stem cell differentiation

Transcriptomic analysis reveals differential gene expression, alternative splicing, and novel exons during mouse trophoblast stem cell differentiation

Expression of tissue factor and thrombomodulin in the placentas of pregnancies affected by early‐onset and late‐onset preeclampsia

Placental Maspin Gene Expression Pattern May Be Used to Distinguish Between Preeclampsia and Intrauterine Growth Restriction

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