Expression of tissue factor and thrombomodulin in the placentas of pregnancies affected by early‐onset and late‐onset preeclampsia

Abstract

Aim To analyze the differential genetic expression and protein localization of thrombomodulin (THBD) and tissue factor (F3) in the placentas of pregnancies affected by preeclampsia. Methods We assessed the expression of THBD and F3 by immunohistochemistry and real‐time polymerase chain reaction (PCR) in placentas from 20 PE cases: 10 early‐onset PE placentas, 10 late‐onset PE placentas, and 10 control cases (normal pregnancies). Results In cases, we found higher THBD and F3 RNA levels in placental tissue. Protein expression in controls differed from that in late‐onset PE placentas, which had lower THBD levels in syncytiotrophoblasts and amniotic cells. Likewise, late‐onset PE placentas exhibited comparatively lower F3 expression in the perivillous fibrin. In contrast, early‐onset PE had high F3 expression in the subdecidual fibrin. We found no significant differences in the F3/THBD ratio between the groups. Conclusion Our study supports evidence that shows the involvement of F3 and THBD in placental disorders. Furthermore, this finding contributes to a better understanding of the physio‐pathological role that these molecules may play in the development of this heterogeneous disease.

Adaptive structural and functional evolution of the placenta protects fetal growth in high elevation deer mice

“…3B; Table S6). In the junctional zone/decidua, we found associations between fetal growth and the expression of fetal hemoglobins Hba-x and Hbb-y (oxygen transport), Plin2 (trophoblast cell survival under hypoxia (45)), and F3 (clotting, pre-eclampsia risk (46)). In the labyrinth zone, the a priori genes for which expression was associated with fetal growth were involved in diverse processes including nutrient transport (i.e., Slc32a2, Slc38a1, Fabp4 ; (47)), vascular growth and function (i.e., Edn1 and Plagl1 ; (48–50)), and trophoblast cell differentiation or survival (i.e., Pappa2, Prkcz , and Mmp28 ; (51–54)).…”

“…In either tissue, several genes associated with variables of interest have clear connections to hypoxia and/or placental development. Genes in the junctional zone/decidua that were associated with fetal growth include the fetal hemoglobins Hba-x and Hbb-y , which are involved in oxygen transport, Plin2 , which is involved in trophoblast cell survival under hypoxia (55), and F3 , a clotting factor connected to vascular signaling pathways and associated with pre-eclampsia cases (56, 57). In the labyrinth zone, we found a priori genes associated with treatment factors of interest that are involved in diverse processes including nutrient transport (i.e., Slc32a2, Slc38a1, Fabp4 ), vascular growth and function (i.e., Edn1 and Plagl1 ), and trophoblast cell differentiation or survival (i.e., Pappa2, Prkcz, Furin , and Mmp28 ).…”

Adaptive structural and functional evolution of the placenta protects fetal growth in high-elevation deer mice

“…3 B and SI Appendix , Table S6 ). In the junctional zone/decidua, we found associations between fetal growth and the expression of fetal hemoglobins Hba-x and Hbb-y (oxygen transport), Plin2 [trophoblast cell survival under hypoxia ( 45 )], and F3 [clotting, preeclampsia risk ( 46 )]. In the labyrinth zone, the a priori genes for which expression was associated with fetal growth were involved in diverse processes including nutrient transport [i.e., Slc32a2 , Slc38a1 , Fabp4 ; ( 47 )], vascular growth and function [i.e., Edn1 and Plagl1 ; ( 48 – 50 )], and trophoblast cell differentiation or survival [i.e., Pappa2 , Prkcz , and Mmp28 ; ( 51 – 54 )].…”