Gene expression of differentiation-specific keratins in oral epithelial dysplasia and squamous cell carcinoma

Bloor, Balvinder K.; Seddon, S.V.; Morgan, Peter

doi:10.1016/s1368-8375(00)00094-4

Cited by 70 publications

(69 citation statements)

References 33 publications

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“…However, its expression mostly disappeared from the whole epithelial layer of CIS. [4][5][6][7][9][10][11] The expression profiles for the keratin genes have previously been demonstrated by using ISH techniques in normal oral mucosa, 19 and by showing their alteration statuses in oral epithelial lesions 20 including oral epithelial dysplasia and SCC. 8 In the present ISH study, we found that the mRNA signals for K13 remained to some extent, but did not completely disappear in the immunohistochemically K13(À) CIS foci, indicating that their translation was suppressed by some posttranscriptional modifications.…”

Section: Discussionmentioning

confidence: 99%

“…[4][5][6][8][9][10][11][12][13][14] Regarding the disappearance of K13 in laryngeal squamous cell carcinomas (SCCs) with definite keratinization tendencies, masking or modification of K13 antigenic sites due to some dysfunction of the K13 gene has been suggested. 11 However, there has been no investigation on the K13 gene expression levels in the oral squamous epithelial lesions lacking the immunohistochemical expression of the K13 protein.…”

Section: Introductionmentioning

confidence: 99%

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Loss of keratin 13 in oral carcinoma in situ: a comparative study of protein and gene expression levels using paraffin sections

et al. 2012

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Immunohistochemical loss of keratin (K)13 is one of the most valuable diagnostic criteria for discriminating carcinoma in situ (CIS) from non-malignancies in the oral mucosa while K13 is stably immunolocalized in the prickle cells of normal oral epithelium. To elucidate the molecular mechanism for the loss of K13, we compared the immunohistochemical profiles for K13 and K16 which is not expressed in normal epithelia, but instead enhanced in CIS, with their mRNA levels by in-situ hybridization in formalin-fixed paraffin sections prepared from 23 CIS cases of the tongue, which were surgically removed. Reverse transcriptase-PCR was also performed using RNA samples extracted from laser-microdissected epithelial fragments of the serial paraffin sections in seven of the cases. Although more enhanced expression levels for K16 were confirmed at both the protein and gene levels in CIS in these seven cases, the loss of K13 was associated with repressed mRNA levels in four cases, but not in the other three cases. The results suggest that the loss of K13 is partly due to its gene repression, but may also be due to some unknown post-translational events.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Loss of keratin 13 in oral carcinoma in situ: a comparative study of protein and gene expression levels using paraffin sections

et al. 2012

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“…CK13 is a differentiation-specific keratin, and Bloor et al reported that its protein expression was reduced in moderate and severe dysplasia (7). Furthermore, the level of CK13 mRNA expression in squamous cell carcinoma and severe dysplasia was reported to be lower than that in mild to moderate dysplasia (8).…”

Section: Discussionmentioning

confidence: 99%

“…The expression of specific types, such as cytokeratin (CK)4 and CK13, is decreased in dysplastic lesions in the oral epithelium, and such changes have been associated with the development of oral dysplasia and squamous cell carcinomas (7,8). On the other hand, Ki-67 is a representative molecular marker of proliferating cells, and its labeling index correlates with the grade of dysplasia and severity of malignancy (9,10).…”

Section: Introductionmentioning

confidence: 99%

Histopathological evaluation including cytokeratin 13 and Ki-67 in the border between Lugol-stained and -unstained areas

Ohta

Ogawa²,

Ono³

et al. 2010

Oncol Rep

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Abstract. Lugol's iodine staining (Lugol-staining) has been widely used to detect malignant changes in the cervix uteri and esophagus. However, pathological and histochemical changes in the border between Lugol-stained and -unstained areas in oral epithelial dysplastia and malignant lesions are not well understood. We examined the histological appearance of 20 cases of epithelial dysplasia surrounding squamous cell carcinoma using HE and PAS staining in the borders between Lugol-stained and -unstained areas. Subsequently, differences in the expression of cytokeratin 13 (CK13), an epithelium differentiation marker, and Ki-67, a cell proliferative marker, in those areas were investigated by immunohistochemistry. Lugol-stained areas of all cases showed mild dysplasia or normal epithelium appearance, while Lugol-unstained areas in most cases were diagnosed as moderate/severe dysplasia and carcinoma in situ. PAS reactions were limited or not found in the Lugol-unstained areas as compared to intense positivity in Lugol-stained areas. CK13 and Ki-67 protein expression was significantly different between Lugol-stained and -unstained areas. It was confirmed that epithelia showing precancerous or cancerous features were detected as Lugol-unstained boundary areas. A reduction in glycogen production caused by alterations of cell differentiation and proliferation associated with malignant changes may result in a lack of Lugol-staining.

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“…In contrast, KRT13 was absent in carcinoma in situ and OSCC. It was suggested that the expression of KRT13 might has a link with epithelial differentiation (Bloor et al, 2001;Mikami et al, 2011). The loss expression of KRT13 expressions had also been reported in cervix carcinoma tissue as the normal tissue progress to become a neoplastic cell (Carrilho et al, 2004).…”

Section: Discussionmentioning

confidence: 94%

KRT13, FAIM2 and CYP2W1 mRNA Expression in Oral Squamous Cell Carcinoma Patients with Risk Habits

Hartanto¹,

Karen-Ng²,

Vincent‐Chong³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Gene expression of differentiation-specific keratins in oral epithelial dysplasia and squamous cell carcinoma

Cited by 70 publications

References 33 publications

Loss of keratin 13 in oral carcinoma in situ: a comparative study of protein and gene expression levels using paraffin sections

Loss of keratin 13 in oral carcinoma in situ: a comparative study of protein and gene expression levels using paraffin sections

Histopathological evaluation including cytokeratin 13 and Ki-67 in the border between Lugol-stained and -unstained areas

KRT13, FAIM2 and CYP2W1 mRNA Expression in Oral Squamous Cell Carcinoma Patients with Risk Habits

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