KRT13, FAIM2 and CYP2W1 mRNA Expression in Oral Squamous Cell Carcinoma Patients with Risk Habits

Hartanto, Firstine Kelsi; Karen-Ng, Lee Peng; Vincent‐Chong, Vui King; Ismail, Siti Mazlipah; Mustafa, Wan Mahadzir Wan; Abraham, Mannil Thomas; Tay, Keng Kiong; Zain, Rosnah Binti

doi:10.7314/apjcp.2015.16.3.953

Cited by 8 publications

(3 citation statements)

References 22 publications

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“…It is a down-regulated gene in cervical carcinoma, and a risk factor that involves in the progress of uncontrolled epithelial cell differentiat,ion. Previous work suggests that the loss of K13 or low K13 mRNA expression is associated with invasive oral squamous cell carcinoma (OSCC) [ 28 , 29 ]. Epigenetic alteration of K13 is one major reason resulting the inhibition of K13 in OSCC.…”

Section: Resultsmentioning

confidence: 99%

KF-finder: identification of key factors from host-microbial networks in cervical cancer

Gao

Zheng

et al. 2018

BMC Syst Biol

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BackgroundThe human body is colonized by a vast number of microbes. Microbiota can benefit many normal life processes, but can also cause many diseases by interfering the regular metabolism and immune system. Recent studies have demonstrated that the microbial community is closely associated with various types of cell carcinoma. The search for key factors, which also refer to cancer causing agents, can provide an important clue in understanding the regulatory mechanism of microbiota in uterine cervix cancer.ResultsIn this paper, we investigated microbiota composition and gene expression data for 58 squamous and adenosquamous cell carcinoma. A host-microbial covariance network was constructed based on the 16s rRNA and gene expression data of the samples, which consists of 259 abundant microbes and 738 differentially expressed genes (DEGs). To search for risk factors from host-microbial networks, the method of bi-partite betweenness centrality (BpBC) was used to measure the risk of a given node to a certain biological process in hosts. A web-based tool KF-finder was developed, which can efficiently query and visualize the knowledge of microbiota and differentially expressed genes (DEGs) in the network.ConclusionsOur results suggest that prevotellaceade, tissierellaceae and fusobacteriaceae are the most abundant microbes in cervical carcinoma, and the microbial community in cervical cancer is less diverse than that of any other boy sites in health. A set of key risk factors anaerococcus, hydrogenophilaceae, eubacterium, PSMB10, KCNIP1 and KRT13 have been identified, which are thought to be involved in the regulation of viral response, cell cycle and epithelial cell differentiation in cervical cancer. It can be concluded that permanent changes of microbiota composition could be a major force for chromosomal instability, which subsequently enables the effect of key risk factors in cancer. All our results described in this paper can be freely accessed from our website at http://www.nwpu-bioinformatics.com/KF-finder/.

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Section: Resultsmentioning

confidence: 99%

KF-finder: identification of key factors from host-microbial networks in cervical cancer

Gao

Zheng

et al. 2018

BMC Syst Biol

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“…KRT13 is a member of the keratin family, which plays a role in cytoskeleton remodeling. KRT13 has been reported to be less expressed in cancerous tissues than in normal tissues in the oral mucosa 19 , 20 . Actually, according to a microarray data from the GEO profiles database (NCBI GEO database, https://www.ncbi.nlm.nih.gov/geo/tools/profileGraph.cgi?ID=GDS1584:207935_s_at , 2022–12-26), 4.5-fold downregulation of KRT13 in OSCC tissues were observed in compared with that in normal oral mucosa.…”

Section: Discussionmentioning

confidence: 99%

KRT13 and UPK1B for differential diagnosis between metastatic lung carcinoma from oral squamous cell carcinoma and lung squamous cell carcinoma

Goda,

Nakashiro,

Sano

et al. 2023

Sci Rep

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Oral squamous cell carcinomas unusually show distant metastasis to the lung after primary treatment, which can be difficult to differentiate from primary squamous cell carcinoma of the lung. While the location and number of tumor nodules is helpful in diagnosing cases, differential diagnosis may be difficult even with histopathological examination. Therefore, we attempted to identify molecules that can facilitate accurate differential diagnosis. First, we performed a comprehensive gene expression analysis using microarray data for OSCC-LM and LSCC, and searched for genes showing significantly different expression levels. We then identified KRT13, UPK1B, and nuclear receptor subfamily 0, group B, member 1 (NR0B1) as genes that were significantly upregulated in LSCC and quantified the expression levels of these genes by real-time quantitative RT-PCR. The expression of KRT13 and UPK1B proteins were then examined by immunohistochemical staining. While OSCC-LM showed no KRT13 and UPK1B expression, some tumor cells of LSCC showed KRT13 and UPK1B expression in 10 of 12 cases (83.3%). All LSCC cases were positive for at least one of these markers. Thus, KRT13 and UPK1B might contribute in differentiating OSCC-LM from LSCC.

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“…In addition, CYP2W1 was also shown to convert arachidonic acid to a number of dihydroxy derivatives albeit at a low conversion rate. In addition, four other fatty acids were also found to be Hartanto et al (2015) hydroxylated and/or epoxidated. In general, all of these reactions occur at low rate, demonstrate K m in the micromolar range and need to be verified under physiological conditions.…”

Section: Substrate Specificitymentioning

confidence: 96%

The CYP2W1 enzyme: regulation, properties and activation of prodrugs

Guo

Johansson

Mkrtchian

et al. 2016

Drug Metabolism Reviews

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CYP2W1 is expressed in the course of development of the gastrointestinal tract, silenced after birth in intestine and colon by epigenetic modifications, but activated following demethylation in colorectal cancer (CRC). The expression levels in CRC positively correlate with the degree of malignancy, are higher in metastases and are predictive of colon cancer survival. The CYP2W1 transcripts have been detected also in hepatocellular carcinoma, adrenocortical carcinoma, childhood rhabdomyosarcoma and breast cancer; however, here the protein expression remains to be confirmed. The CYP2W1 enzyme has an inverted orientation in the endoplasmic reticulum membrane, as compared to other cytochrome P450s and its immediate electron donor is unknown. Several lipid ligands have been proposed as endogenous substrates, among which retinol derivatives appear to have the highest affinities. However, the role of CYP2W1 in the endogenous and tumor localized metabolism of retinol derivatives has yet to be clarified. Indolines constitute high affinity exogenous compounds and specific chloromethylindolines have been shown to be activated by CYP2W1 into cytotoxic products in vitro and also in vivo, inhibiting the growth of human colon tumors in a mouse xenograft model. The CRC specific localization of CYP2W1 and its effective prodrug activation makes it a very promising target for future development of cancer therapeutics.

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KRT13, FAIM2 and CYP2W1 mRNA Expression in Oral Squamous Cell Carcinoma Patients with Risk Habits

Cited by 8 publications

References 22 publications

KF-finder: identification of key factors from host-microbial networks in cervical cancer

KF-finder: identification of key factors from host-microbial networks in cervical cancer

KRT13 and UPK1B for differential diagnosis between metastatic lung carcinoma from oral squamous cell carcinoma and lung squamous cell carcinoma

The CYP2W1 enzyme: regulation, properties and activation of prodrugs

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