Gene expression modulation in chicken macrophages exposed to Mycoplasma synoviae or Escherichia coli

Lavrič, Miha; Maughan, Michele N.; Bliss, Travis W.; Dohms, John E.; Benčina, Dušan; Keeler, Calvin L.; Narat, Mojca

doi:10.1016/j.vetmic.2007.06.011

Cited by 50 publications

(36 citation statements)

References 26 publications

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“…Many types of chicken microarrays have been used in genome-wide gene expression studies, including a macrophage microarray, avian innate immunity microarray, 44 K Agilent microarray, and Affymetrix chicken genome array (Call et al., 2001; Lavric et al., 2008; Li et al., 2008; Kranis et al., 2013). The new technology of RNAseq is an efficient and reliable tool to investigate genetic architecture and sequence variation and to quantify gene expression through whole transcriptome analysis (Ozsolak and Milos, 2011).…”

Section: Introductionmentioning

confidence: 99%

Thymus transcriptome reveals novel pathways in response to avian pathogenic Escherichia coli infection

et al. 2016

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Avian pathogenic Escherichia coli (APEC) can cause significant morbidity in chickens. The thymus provides the essential environment for T cell development; however, the thymus transcriptome has not been examined for gene expression in response to APEC infection. An improved understanding of the host genomic response to APEC infection could inform future breeding programs for disease resistance and APEC control. We therefore analyzed the transcriptome of the thymus of birds challenged with APEC, contrasting susceptible and resistant phenotypes. Thousands of genes were differentially expressed in birds of the 5-day post infection (dpi) challenged-susceptible group vs. 5 dpi non-challenged, in 5 dpi challenged-susceptible vs. 5 dpi challenged-resistant birds, as well as in 5 dpi vs. one dpi challenged-susceptible birds. The Toll-like receptor signaling pathway was the major innate immune response for birds to respond to APEC infection. Moreover, lysosome and cell adhesion molecules pathways were common mechanisms for chicken response to APEC infection. The T-cell receptor signaling pathway, cell cycle, and p53 signaling pathways were significantly activated in resistant birds to resist APEC infection. These results provide a comprehensive assessment of global gene networks and biological functionalities of differentially expressed genes in the thymus under APEC infection. These findings provide novel insights into key molecular genetic mechanisms that differentiate host resistance from susceptibility in this primary lymphoid tissue, the thymus.

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Section: Introductionmentioning

confidence: 99%

Thymus transcriptome reveals novel pathways in response to avian pathogenic Escherichia coli infection

et al. 2016

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“…Information about the secretome of chicken chondrocytes is scarce. Although various cytokines including IL-1 β , IL-18 nitric oxide, IFN α , and IFN γ have been found in the synovial fluid of infected chickens (Dusanic et al, unpublished observations) these are also secreted by activated immune cells including macrophages [7, 8]. Since cytokines act as a network, the potential ability of CCH to secrete certain cytokines could, in combination with the ones already present in wells of phenotype microarrays, influence the overall effect on chondrocytes, leading to a significant reduction in the expression of anabolic genes (e.g., aggrecan, collagen type II), upregulation of various catabolic genes (e.g., matrix degrading proteases) and a strong induction of intercellular mediators (e.g., leukemia inhibitory factor, IL-6).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic Characterization ofMycoplasma synoviaeInduced Changes in the Metabolic and Sensitivity Profile ofIn VitroInfected Chicken Chondrocytes

Dušanić

Benčina

Narat

et al. 2014

BioMed Research International

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In infectious synovitis caused by Mycoplasma synoviae chicken chondrocytes (CCH) may come into direct contact with these bacteria that are also capable of invading CCH in vitro. In this study, phenotype microarrays were used to evaluate the influence of Mycoplasma synoviae on the global metabolic activity of CCH. Therefore, CCH were cultured in the presence of 504 individual compounds, spotted in wells of 11 phenotype microarrays for eukaryotic cells, and exposed to Mycoplasma synoviae membranes or viable Mycoplasma synoviae. Metabolic activity and sensitivity of normal cells versus infected cells were evaluated. Metabolic profiles of CCH treated with viable Mycoplasma synoviae or its membranes were significantly different from those of CCH alone. CCH treated with Mycoplasma synoviae membranes were able to use 48 carbon/nitrogen sources not used by CCH alone. Treatment also influenced ion uptake in CCH and intensified the sensitivity to 13 hormones, 5 immune mediators, and 29 cytotoxic chemicals. CCH were even more sensitive to hormones/immune mediators when exposed to viable Mycoplasma synoviae. Our results indicate that exposure to Mycoplasma synoviae or its membranes induces a wide range of metabolic and sensitivity modifications in CCH that can contribute to pathological processes in the development of infectious synovitis.

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“…Our research also suggested FcCathL role in shrimp anti-WSSV innate immunity. CathL gene was upregulated by 5.7 fold in monocyte-derived macrophages (MDM) under 24 h avian pathogenic E. coli strain (APEC) [27]. CathL was upregulated in Chinese soft-shelled turtle (Trionyx sinensis) infected with Aeromonas hydrophila, a ubiquitous waterborne bacterium [28].…”

Section: Discussionmentioning

confidence: 99%

Two cysteine proteinases respond to bacterial and WSSV challenge in Chinese white shrimp Fenneropenaeus chinensis

Ren

Zhang

Sun

et al. 2010

Fish & Shellfish Immunology

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Gene expression modulation in chicken macrophages exposed to Mycoplasma synoviae or Escherichia coli

Cited by 50 publications

References 26 publications

Thymus transcriptome reveals novel pathways in response to avian pathogenic Escherichia coli infection

Thymus transcriptome reveals novel pathways in response to avian pathogenic Escherichia coli infection

Phenotypic Characterization ofMycoplasma synoviaeInduced Changes in the Metabolic and Sensitivity Profile ofIn VitroInfected Chicken Chondrocytes

Two cysteine proteinases respond to bacterial and WSSV challenge in Chinese white shrimp Fenneropenaeus chinensis

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