Two cysteine proteinases respond to bacterial and WSSV challenge in Chinese white shrimp Fenneropenaeus chinensis

Ren, Qian; Zhang, Xiaowen; Sun, Yuan; Sun, Shanshan; Zhou, Jing; Wang, Zong-Heng; Zhao, Xiao‐Fan; Wang, Jinxing

doi:10.1016/j.fsi.2010.03.002

Cited by 35 publications

(9 citation statements)

References 37 publications

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“…Similar functions of CatL in vitellogenesis were also found in rainbow trout Oncorhynchus mykiss (Kwon et al, 2001) and Danio rerio (Carnevali et al, 2006). Most studies on CatL in arthropods have focused on the function of CatL in immunity (Ren et al, 2010;Zhang et al, 2010b) and digestion (Hu and Leung, 2007;Soares-Costa et al, 2011), but little is known of its role in VTG utilization. Current evidence in Metapenaeus ensis (Crustacean) suggests that the ovary is the only non-alimentary organ showing CatL expression (Hu and Leung, 2004).…”

Section: Introductionmentioning

confidence: 65%

Molecular characterization of cathepsin L cDNA and its expression during oogenesis and embryogenesis in the oriental river prawn Macrobrachium nipponense (Palaemonidae)

Zhao¹,

Chen²,

Zhang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. We identified the cDNA sequence of cathepsin L (CatL) in Macrobrachium nipponense, designated as MnCatL, for the first time. The MnCatL cDNA, isolated from the ovary, was 1710 bp in length, containing a 31-bp 5'-untranslated region, a 650-bp 3'-untranslated region, and an open reading frame of 1029 bp, encoding a 342-amino acid polypeptide with a predicted molecular mass of 37.7 kDa. The polypeptide is composed of an 18-amino acid signal peptide, a 106-amino acid propeptide and a 218-amino acid mature peptide. MnCatL mRNA was detected in all tissues that we examined, including the thoracic ganglia, heart, muscle, intestine, hemocytes, ovary, testis, gills, and hepatopancreas. MnCatL expression reached a maximum value in both hepatopancreas and ovaries at the later stages of vitellogenesis, suggesting that MnCatL is involved in ovarian maturation of the oriental river prawn. During embryogenesis, MnCatL expression decreased as the embryo developed. The expression of MnCatL in the ovary and embryo suggest that MnCatL plays an important role in the uptake of vitellogenin and yolk protein, which are deposited in the oocyte for ovary maturation and embryo development, during oogenesis and embryogenesis of M. nipponense.

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Section: Introductionmentioning

confidence: 65%

Molecular characterization of cathepsin L cDNA and its expression during oogenesis and embryogenesis in the oriental river prawn Macrobrachium nipponense (Palaemonidae)

Zhao¹,

Chen²,

Zhang³

et al. 2013

Genet. Mol. Res.

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“…In humans, the clan CA papain proteinases are reported to be involved in the MHC class II antigen‐processing pathway (Murray et al ., ). A cathepsin L in shrimp is also reported to be responsive to viral challenge (Robalino et al ., ; Zhao et al ., ; Ren et al ., ). In the present study, the SlCyp transcript was strongly induced in all the tissues tested in N. rileyi ‐infected S. litura , but the strongest induction was in the haemocytes, followed by the fat bodies.…”

Section: Discussionmentioning

confidence: 97%

Structure and expression of a cysteine proteinase gene from Spodoptera litura and its response to biocontrol fungus Nomuraea rileyi

Chen

Yin

Feng

et al. 2014

Insect Molecular Biology

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Cysteine proteinases (Cyps) play vital roles in many biological processes, including physiological and pathological reactions. In the present study, we cloned a full cDNA of SlCyp, encoding a 344-amino-acid protein from Spodoptera litura. The putative amino acid sequence shared >75% identity with Cyps from other insects. A phylogenetic analysis revealed that SlCyp is closely related to other known lepidopteran Cyps. Real-time PCR and Western blotting analyses showed that SlCyp is induced by Nomuraea rileyi infection in all the tissues tested. The strongest SlCyp mRNA and protein expression was found in haemocytes, followed by the fat bodies, of unchallenged and N. rileyi-challenged S. litura. A time-course analysis showed that SlCyp mRNA and protein expression levels were upregulated in the haemocytes and fat bodies by N. rileyi infection. Upon N. rileyi infection, the proteolytic activities of SlCyp were also significantly higher in the haemolymph than in normal or phosphate-buffered-saline-challenged controls. These results suggest that SlCyp plays an important role in the innate immunity of S. litura in response to N. rileyi. SlCyp mRNA and protein expression and activities were also elevated during sixth-instar moulting and metamorphosis. Knocking down SlCyp transcripts with double-stranded RNA interference caused prepupal, pupal, and adult phenotypic changes, and SlCyp-silenced mutant larvae displayed a significantly lower survival rate after N. rileyi infection. These facts suggest that SlCyp plays a significant role in resisting N. rileyi infection and an essential role in larval development. Our data should facilitate the development of techniques for S. litura control.

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“…A proteomic approach for hemocytes-like cell culture of Drosophila reveal the presence of several cysteine proteases but it's role is still unclear [45]. In the Chinese shrimp Fenneropenaeus chinesis a cathepsin L like proteases were up-regulated during WSSV (White Spot syndrome Virus) and bacteria (Vibrio) challenge [46], also the transcriptome shows that several cysteine proteases are up-regulated during infection [47]. Taking all our data together with other studies [48e50] reinforce the role of cysteine proteases and probable their inhibitor such as cystatin in invertebrate immunity.…”

Section: Discussionmentioning

confidence: 99%

Rmcystatin3, a cysteine protease inhibitor from Rhipicephalus microplus hemocytes involved in immune response

Soares

Vaz

et al. 2014

Biochimie

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The Rhipicephalus microplus tick is responsible for losses in the livestock production estimated in 2 billions USD. Despite its economical importance the knowledge in tick's physiology is sparse. In order to contribute to this scenario we describe the characterization of a cysteine proteinase inhibitor named Rmcystatin-3. Purified recombinant Rmcystatin-3 was able to inhibit cathepsin L (Ki = 2.5 nM), BmCl1 (Ki = 1.8 nM) and cathepsin B (Ki = 136 nM). Western blot and quantitative PCR analysis revealed the presence of Rmcystatin-3 in fat body, salivary gland but mainly in hemocytes. The mRNA levels of Rmcystatin-3 during bacterial challenge are drastically down-regulated. In order to define the Rmcystatin-3 possible role in tick immunity, the cystatin gene was knockdown by RNA interference with and without Escherichia coli infection. Our results showed that the Rmcystatin-3 silenced group was more immune competent to control bacterial infection than the group injected with non-related dsRNA. Taking together, our data strongly suggested an important role of Rmcystatin-3 in tick immunity.

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Two cysteine proteinases respond to bacterial and WSSV challenge in Chinese white shrimp Fenneropenaeus chinensis

Cited by 35 publications

References 37 publications

Molecular characterization of cathepsin L cDNA and its expression during oogenesis and embryogenesis in the oriental river prawn Macrobrachium nipponense (Palaemonidae)

Molecular characterization of cathepsin L cDNA and its expression during oogenesis and embryogenesis in the oriental river prawn Macrobrachium nipponense (Palaemonidae)

Structure and expression of a cysteine proteinase gene from Spodoptera litura and its response to biocontrol fungus Nomuraea rileyi

Rmcystatin3, a cysteine protease inhibitor from Rhipicephalus microplus hemocytes involved in immune response

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