Gene expression in peri‐implant crevicular fluid of smokers and nonsmokers. 1. The early phase of osseointegration

Sayardoust, Shariel; Omar, Omar; Thomsen, Peter

doi:10.1111/cid.12486

Cited by 19 publications

(34 citation statements)

References 36 publications

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“…Recently, Sayardoust et al showed that smoking is associated with different gene profiles post‐surgery for dental implants 37. Here, we observed that, with intact skin, no gene expression differences were observed.…”

Section: Discussionsupporting

confidence: 51%

Cytokine expression profile in the bone‐anchored hearing system: 12‐week results from a prospective randomized, controlled study

Calon

Tongeren

Omar

et al. 2018

Clin Implant Dent Rel Res

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ObjectiveTo study the effect of implanting the percutaneous bone‐anchored hearing system (BAHS) itself and inflammation of the peri‐abutment skin warrant clarification. In this study, we aimed to acquire further insight into the immune responses related to BAHS surgery and peri‐implant skin inflammation.Materials and MethodsDuring surgery and 12 weeks post‐implantation, skin biopsies were obtained. If applicable, additional biopsies were taken during cases of inflammation. The mRNA expression of IL‐1β, IL‐6, IL‐8, TNFα, IL‐17, IL‐10, TGF‐ß, MIP‐1α, MMP‐9, TIMP‐1, COL1α1, VEGF‐A, FGF‐2 TLR‐2, and TLR‐4 was quantified using qRT‐PCR.ResultsThirty‐five patients agreed to the surgery and 12‐week biopsy. Twenty‐two patients had mRNA of sufficient quality for analysis. Ten were fitted with a BAHS using the minimally invasive Ponto surgery technique. Twelve were fitted with a BAHS using the linear incision technique with soft‐tissue preservation. Five biopsies were obtained during episodes of inflammation. The post‐implantation mRNA expression of IL‐1β (P = .002), IL‐8 (P = .003), MMP9 (P = .005), TIMP‐1 (P = .002), and COL1α1 (P < .001) was significantly up‐regulated. IL‐6 (P = .009) and FGF‐2 (P = .004) mRNA expression was significantly down‐regulated after implantation. Within patients, no difference between post‐implantation mRNA expression (at 12 weeks) and when inflammation was observed. Between patients, the expression of IL‐1β (P = .015) and IL‐17 (P = .02) was higher during cases of inflammation compared with patients who had no inflammation at 12‐week follow‐up.ConclusionsAs part of a randomized, prospective, clinical trial, the present study reports the molecular profile of selected cytokines in the soft tissue around BAHS. Within the limit of this study, the results showed that 12 weeks after BAHS implantation the gene expression of some inflammatory cytokines (IL‐8 and IL‐1β) is still relatively high compared with the baseline, steady‐state, expression. The up‐regulation of anabolic (COL1α1) and tissue‐remodeling (MMP‐9 and TIMP1) genes indicates an ongoing remodeling process after 12 weeks of implantation. The results suggest that IL‐1β, IL‐17, and TNF‐α may be interesting markers associated with inflammation.

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Section: Discussionsupporting

confidence: 51%

Cytokine expression profile in the bone‐anchored hearing system: 12‐week results from a prospective randomized, controlled study

Calon

Tongeren

Omar

et al. 2018

Clin Implant Dent Rel Res

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“…Immediately after implant insertion, maximum pain was perceived and it gradually decreased to a low level in both smokers and non‐smokers . On the other hand, the few patients with early (< 28 days) implant failure had a significantly higher perception of pain . Here, we show that the perception of pain is very low at 60 days and non‐existent at 90 days, indicating that treatment with dental implants is predictable and associated with low morbidity.…”

Section: Discussionmentioning

confidence: 57%

“…No implant losses were registered between the latest follow‐up time (28 days) of the early period of healing up to 90 days.…”

Section: Resultsmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

“…One approach is to employ the non‐destructive sampling of cells in the peri‐implant crevicular fluid (PICF) in combination with the clinical evaluations, thereby allowing detailed comparative and correlative analyses of the molecular activities and the soft‐ and hard‐tissue conditions around clinical implants in smokers and non‐smokers. Using this approach, a recent study of the early phase of osseointegration, up to 28 days, revealed few early losses and similar implant survival and few differences in the gene expression in PICF cells between smokers and non‐smokers . On the other hand, differences in the bone composition and (ultra)structure were indicated, based on the observation of higher implant stability quotient (ISQ) (indicator of implant stability in bone) values, which correlated to recipient bone quality and quantity in smokers.…”

Section: Introductionmentioning

confidence: 99%

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Clinical, radiological, and gene expression analyses in smokers and non‐smokers, Part 2: RCT on the late healing phase of osseointegration

Sayardoust

Omar

Norderyd

et al. 2017

Clin Implant Dent Rel Res

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During the early phase of osseointegration, non-smokers and smokers present a similar, high implant survival. In contrast, smokers present a greater MBL, particularly at machined implants. HIF-1α baseline expression in the recipient bone and IL-6 expression in PICF cells are important molecular determinants for MBL after 90 days. It is concluded that smoking has an early effect on osseointegration, which is dependent on the implant surface properties and the local host response.

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Does Smoking Impair Bone Regeneration in the Dental Alveolar Socket?

et al. 2019

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Smoking is a major risk factor for dental implant failure. In addition to higher marginal bone loss around implants, the cellular and molecular responses to injury and implant physicochemical properties are also differentially affected in smokers. The purpose of this work is to determine if smoking impairs bone microstructure and extracellular matrix composition within the dental alveolar socket after tooth extraction. Alveolar bone biopsies obtained from Smokers (> 10 cigarettes per day for at least 10 years) and Ctrl (never-smokers), 7-146 months after tooth extraction, were investigated using X-ray micro-computed tomography, backscattered electron scanning electron microscopy, and Raman spectroscopy. Both Smokers and Ctrl exhibited high inter-and intra-individual heterogeneity in bone microstructure, which varied between dense cortical and porous trabecular architecture. Regions of disorganised/woven bone were more prevalent during early healing. Remodelled lamellar bone was predominant at longer healing periods. Bone mineral density, bone surface-to-volume ratio, mineral crystallinity, the carbonate-to-phosphate ratio, the mineral-to-matrix ratio, the collagen crosslink ratio, and the amounts of amino acids phenylalanine and proline/hydroxyproline were also comparable between Smokers and Ctrl. Bone microstructure and composition within the healing dental alveolar socket are not significantly affected by moderate-to-heavy smoking.

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Gene expression in peri‐implant crevicular fluid of smokers and nonsmokers. 1. The early phase of osseointegration

Cited by 19 publications

References 36 publications

Cytokine expression profile in the bone‐anchored hearing system: 12‐week results from a prospective randomized, controlled study

Cytokine expression profile in the bone‐anchored hearing system: 12‐week results from a prospective randomized, controlled study

Clinical, radiological, and gene expression analyses in smokers and non‐smokers, Part 2: RCT on the late healing phase of osseointegration

Does Smoking Impair Bone Regeneration in the Dental Alveolar Socket?

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