Gene Expression during Survival of<i>Escherichia coli</i>O157:H7 in Soil and Water

Duffitt, Ashley D.; Reber, Robert T.; Whipple, Andrew P.; Chauret, Christian

doi:10.1155/2011/340506

Cited by 30 publications

(23 citation statements)

References 34 publications

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“…In recent years, the advent of modern and high-throughput molecular tools has given researchers an opportunity to examine these microorganisms in their natural environments (14). These techniques could be applied to obtain an understanding of the intimate interactions between food-borne pathogens and vegetable plants that have served as vehicles of transmission.…”

mentioning

confidence: 99%

“…Microarray technologies have recently been applied to study the interaction of food-borne pathogens with food matrices, such as vegetables, meats, and dairy foods (2,17,36). However, genomic approaches to identify and monitor the genes involved in bacterial survival in the environment associated with vegetable production have been used in only a few studies (14,36). Kyle et al (36) conducted one of the first genome-wide studies on the interaction of E. coli O157:H7 with lettuce.…”

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confidence: 99%

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Transcriptional Responses of Escherichia coli K-12 and O157:H7 Associated with Lettuce Leaves

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Hou

et al. 2012

Appl Environ Microbiol

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An increasing number of outbreaks of gastroenteritis recently caused by Escherichia coli O157:H7 have been linked to the consumption of leafy green vegetables. Although it is known that E. coli survives and grows in the phyllosphere of lettuce plants, the molecular mechanisms by which this bacterium associates with plants are largely unknown. The goal of this study was to identify E. coli genes relevant to its interaction, survival, or attachment to lettuce leaf surfaces, comparing E. coli K-12, a model system, and E. coli O157:H7, a pathogen associated with a large number of outbreaks. Using microarrays, we found that upon interaction with intact leaves, 10.1% and 8.7% of the 3,798 shared genes were differentially expressed in K-12 and O157:H7, respectively, whereas 3.1% changed transcript levels in both. The largest group of genes downregulated consisted of those involved in energy metabolism, including tnaA (33-fold change), encoding a tryptophanase that converts tryptophan into indole. Genes involved in biofilm modulation (bhsA and ybiM) and curli production (csgA and csgB) were significantly upregulated in E. coli K-12 and O157:H7. Both csgA and bhsA (ycfR) mutants were impaired in the long-term colonization of the leaf surface, but only csgA mutants had diminished ability in short-term attachment experiments. Our data suggested that the interaction of E. coli K-12 and O157:H7 with undamaged lettuce leaves likely is initiated via attachment to the leaf surface using curli fibers, a downward shift in their metabolism, and the suppression of biofilm formation.

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confidence: 99%

mentioning

confidence: 99%

Transcriptional Responses of Escherichia coli K-12 and O157:H7 Associated with Lettuce Leaves

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Hou

et al. 2012

Appl Environ Microbiol

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“…Loss of flagella in Rhizobium meliloti was reported to occur during starvation (40). EPS and capsular polysaccharide (CPS) produc-tion also were influenced by environmental conditions (9,30). Curli are typically expressed under stress conditions, such as low growth temperature (below 30°C) and nutrient limitations (27).…”

Section: Resultsmentioning

confidence: 99%

Influence of the Plant Defense Response to Escherichia coli O157:H7 Cell Surface Structures on Survival of That Enteric Pathogen on Plant Surfaces

Seo

Matthews

2012

Appl Environ Microbiol

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ABSTRACTConsumption of fresh and fresh-cut fruits and vegetables contaminated withEscherichia coliO157:H7 has resulted in hundreds of cases of illness and, in some instances, death. In this study, the influence of cell surface structures ofE. coliO157:H7, such as flagella, curli fimbriae, lipopolysaccharides, or exopolysaccharides, on plant defense responses and on survival or colonization on the plant was investigated. The population of theE. coliO157:H7 ATCC 43895 wild-type strain was significantly lower on wild-typeArabidopsisplants than that of the 43895 flagellum-deficient mutant. The population of theE. coliO157:H7 43895 flagellum mutant was greater on both wild-type andnpr1-1mutant (nonexpressor of pathogenesis-related [PR] genes) plants and resulted in less PR gene induction, estimated based on a weak β-glucuronidase (GUS) signal, than did the 43895 wild-type strain. These results suggest that the flagella, among the other pathogen-associated molecular patterns (PAMPs), made a substantial contribution to the induction of plant defense response and contributed to the decreased numbers of theE. coliO157:H7 ATCC 43895 wild-type strain on the wild-typeArabidopsisplant. A curli-deficientE. coliO157:H7 86-24 strain survived better on wild-typeArabidopsisplants than the curli-producing wild-type 86-24 strain did. The curli-deficientE. coliO157:H7 86-24 strain exhibited a GUS signal at a level substantially lower than that of the curli-producing wild-type strain. Curli were recognized by plant defense systems, consequently affecting bacterial survival. The cell surface structures ofE. coliO157:H7 have a significant impact on the induction of differential plant defense responses, thereby impacting persistence or survival of the pathogen on plants.

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“…This gene is the major cold shock protein of E. coli and belongs to a family of structurally analogous proteins. It is an RNA chaperone to prevent the misfolding of single-stranded RNAs due to the formation of secondary structures at cold temperatures [33,34]. Cold shock, however, was prevented as much as possible in the present study, as the MOPS preculture, the MgSO 4 inoculum and the temperature of the growth chamber were incubated or set at 18˝C.…”

Section: Stress Responsive Genesmentioning

confidence: 99%

Microarray-Based Screening of Differentially Expressed Genes of E. coli O157:H7 Sakai during Preharvest Survival on Butterhead Lettuce

Linden

Cottyn²,

Uyttendaele

et al. 2016

Agriculture

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Numerous outbreaks of Escherichia coli O157:H7 have been linked to the consumption of leafy vegetables. However, up to the present, little has been known about E. coli O157:H7's adaptive responses to survival on actively growing (and thus responsive) plants. In this study, whole genome transcriptional profiles were generated from E. coli O157:H7 cells (isolate Sakai, stx-) one hour and two days after inoculation on the leaves of growing butterhead lettuce, and compared with an inoculum control. A total of 273 genes of E. coli O157:H7 Sakai (5.04% of the whole genome) were significantly induced or repressed by at least two-fold (p < 0.01) in at least one of the analyzed time points in comparison with the control. Several E. coli O157:H7 genes associated with oxidative stress and antimicrobial resistance were upregulated, including the iron-sulfur cluster and the multiple antibiotic resistance (mar) operon, whereas the Shiga toxin virulence genes were downregulated. Nearly 40% of the genes with significantly different expression were poorly characterized genes or genes with unknown functions. These genes are of special interest for future research as they may play an important role in the pathogens' adaptation to a lifestyle on plants. In conclusion, these findings suggest that the pathogen actively interacts with the plant environment by adapting its metabolism and responding to oxidative stress.

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Gene Expression during Survival ofEscherichia coliO157:H7 in Soil and Water

Cited by 30 publications

References 34 publications

Transcriptional Responses of Escherichia coli K-12 and O157:H7 Associated with Lettuce Leaves

Transcriptional Responses of Escherichia coli K-12 and O157:H7 Associated with Lettuce Leaves

Influence of the Plant Defense Response to Escherichia coli O157:H7 Cell Surface Structures on Survival of That Enteric Pathogen on Plant Surfaces

Microarray-Based Screening of Differentially Expressed Genes of E. coli O157:H7 Sakai during Preharvest Survival on Butterhead Lettuce

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