Influence of the Plant Defense Response to Escherichia coli O157:H7 Cell Surface Structures on Survival of That Enteric Pathogen on Plant Surfaces

Seo, Suengwook; Matthews, Karl R.

doi:10.1128/aem.01095-12

Cited by 41 publications

(33 citation statements)

References 43 publications

(48 reference statements)

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“…Plants were grown from seeds under conditions that mimic those in commercial lettuce production greenhouses in Belgium; the bacterial strain used for inoculations, E. coli O157:H7 Sakai, previously isolated from a fresh produce outbreak, was cultured at 18˝C and resuspended in a low concentration salt solution to simulate the suboptimal environmental conditions that occur outside the animal host, e.g., in irrigation water. The results support the concept that the plant environment, or, more broadly, the non-animal environment, is part of the ecological niche of human pathogens [66][67][68][69][70]. Firstly, the data showed that genes such as the highly upregulated azoR could have been induced in reaction to the plant defense response.…”

Section: Discussionsupporting

confidence: 78%

Microarray-Based Screening of Differentially Expressed Genes of E. coli O157:H7 Sakai during Preharvest Survival on Butterhead Lettuce

Linden

Cottyn²,

Uyttendaele

et al. 2016

Agriculture

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Numerous outbreaks of Escherichia coli O157:H7 have been linked to the consumption of leafy vegetables. However, up to the present, little has been known about E. coli O157:H7's adaptive responses to survival on actively growing (and thus responsive) plants. In this study, whole genome transcriptional profiles were generated from E. coli O157:H7 cells (isolate Sakai, stx-) one hour and two days after inoculation on the leaves of growing butterhead lettuce, and compared with an inoculum control. A total of 273 genes of E. coli O157:H7 Sakai (5.04% of the whole genome) were significantly induced or repressed by at least two-fold (p < 0.01) in at least one of the analyzed time points in comparison with the control. Several E. coli O157:H7 genes associated with oxidative stress and antimicrobial resistance were upregulated, including the iron-sulfur cluster and the multiple antibiotic resistance (mar) operon, whereas the Shiga toxin virulence genes were downregulated. Nearly 40% of the genes with significantly different expression were poorly characterized genes or genes with unknown functions. These genes are of special interest for future research as they may play an important role in the pathogens' adaptation to a lifestyle on plants. In conclusion, these findings suggest that the pathogen actively interacts with the plant environment by adapting its metabolism and responding to oxidative stress.

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Section: Discussionsupporting

confidence: 78%

Microarray-Based Screening of Differentially Expressed Genes of E. coli O157:H7 Sakai during Preharvest Survival on Butterhead Lettuce

Linden

Cottyn²,

Uyttendaele

et al. 2016

Agriculture

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“…However, LPS-dependent responses seem not to be sufficient to restrict bacterial survival on plants as the population titer of E. coli O157:H7 LPS mutant or wild type in plant is essentially the same (Seo and Matthews, 2012). Additionally, live S .…”

Section: Perception Of Human Pathogens By the Plant Immune Systemmentioning

confidence: 99%

Plant innate immunity against human bacterial pathogens

2014

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Certain human bacterial pathogens such as the enterohemorrhagic Escherichia coli and Salmonella enterica are not proven to be plant pathogens yet. Nonetheless, under certain conditions they can survive on, penetrate into, and colonize internal plant tissues causing serious food borne disease outbreaks. In this review, we highlight current understanding on the molecular mechanisms of plant responses against human bacterial pathogens and discuss salient common and contrasting themes of plant interactions with phytopathogens or human pathogens.

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“…An exhaustive study involving the screening of a mutant library of L. monocytogenes and identification of three mutants that had reduced adherence to radish tissue suggested that temperature may affect expression of attachment factors used by L. monocytogenes (Gorski et al, 2003). A series of experiments were conducted to determine the influence of cell surface structures of E. coli O157:H7, such as flagella, curli, lipopolysaccharide, and exopolysaccharide on survival and colonization of the pathogen on plant leaf tissue (Seo and Matthews, 2012). The research demonstrated that flagella contributed to the induction of plant defense response.…”

Section: Microbe Characteristicsmentioning

confidence: 99%