Gene expression and enzyme activity of pepper (Capsicum annuum L.) ascorbate oxidase during elicitor and wounding stress

García-Pineda, Ernesto

doi:10.1016/j.plantsci.2003.09.013

Cited by 31 publications

(17 citation statements)

References 37 publications

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“…The ascorbate oxidase enzyme activity was measured according to a modified version of the method of García‐Pineda et al 39 based on the fact that ascorbate absorbs at 265 nm whereas the oxidation product, dehydroascorbate, does not. The reaction mixture (1 mL) consisted of 0.025 mol L −1 citrate/phosphate buffer (pH 5.6), 0.08 mmol L −1 L ‐ascorbic acid, 0.02 mmol L −1 neutralized disodium EDTA and 0.15 g L −1 bovine serum albumin solution and up to 200 µL of enzyme extract (extinction coefficient = 9246 L mol −1 cm −1 at 265 nm).…”

Section: Methodsmentioning

confidence: 99%

Antioxidant content and ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation

et al. 2006

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Considering the economic importance of the tomato and its nutritional benefits to human health, a study was made of how two different environmental factors (temperature and overall solar radiation) influence the nutritional quality of cherry tomatoes during the plant full production cycle. Solanum lycopersicum L. cv. Naomi plants were grown in an experimental greenhouse. Three fruit samples were taken over the full production period: first sampling at the beginning of harvest (7 January 2004), second at mid-harvest (22 March 2004) and third at harvest end (30 May 2004). Values for temperature and overall accumulated solar radiation peaked at a maximum in the third sampling, without lowering the yield with respect to previous samplings. Regarding the antioxidant activity in the exocarp fraction of the cherry tomato fruits, the results showed that the increase in temperature and solar radiation diminished the lycopene and β-carotene contents in the third sampling, inducing defective pigmentation (sunscald). This occurred simultaneously with an increase in lipid peroxidation during the third sampling, quantified as lipoxygenase activity and malondialdehyde content. Finally, in relation to ascorbate metabolism, the higher temperatures and stronger solar radiation at the third sampling increased the oxidation of reduced ascorbate (AsA) due to intensified ascorbate peroxidase (APX) and ascorbate oxidase (AO) activities and a depression of the enzyme dehydroascorbate reductase (DHAR). In conclusion, the results indicate that despite the oxidation of AsA by APX and AO, the minimal regeneration of the latter, together with the greater lipid peroxidation with increasing temperature and solar radiation in the greenhouse, explained the lower content of antioxidants in the exocarp and therefore the loss of nutritional quality of the cherry tomato fruits grown under these conditions.

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Section: Methodsmentioning

confidence: 99%

Antioxidant content and ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation

et al. 2006

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“…Specifically,eicosapentaenoic acid (EPA;20:5 D 5,8,11,14,17 ) and arachidonic acid (AA;20:4 D 5,8,11,14 ) are potent elicitors of programmed cell death and defense responses in Solanaceous plants (Bostock et al, 1981;Knight et al, 2001;Garcia-Pineda et al, 2004) and are reported to induce resistance to viruses in potato (Solanum tuberosum) and tobacco (Nicotiana tabacum) (Rozhnova et al, 2003;Ozeretskovskaya et al, 2004). Treatment of potato with AA and EPA results in the coordinate activation of defense-related responses (Bostock et al, 1986) and a dramatic and immediate shift in terpenoid metabolism, redirecting the pathway from higher terpenoids (i.e., steroid glycoalkaloids) to sesquiterpenoid phytoalexins (Tjamos and Kuć , 1982;Choi et al, 1992Choi et al, , 1994.…”

Section: Introductionmentioning

confidence: 99%

Arachidonic Acid: An Evolutionarily Conserved Signaling Molecule Modulates Plant Stress Signaling Networks

et al. 2010

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Fatty acid structure affects cellular activities through changes in membrane lipid composition and the generation of a diversity of bioactive derivatives. Eicosapolyenoic acids are released into plants upon infection by oomycete pathogens, suggesting they may elicit plant defenses. We exploited transgenic Arabidopsis thaliana plants (designated EP) producing eicosadienoic, eicosatrienoic, and arachidonic acid (AA), aimed at mimicking pathogen release of these compounds. We also examined their effect on biotic stress resistance by challenging EP plants with fungal, oomycete, and bacterial pathogens and an insect pest. EP plants exhibited enhanced resistance to all biotic challenges, except they were more susceptible to bacteria than the wild type. Levels of jasmonic acid (JA) were elevated and levels of salicylic acid (SA) were reduced in EP plants. Altered expression of JA and SA pathway genes in EP plants shows that eicosapolyenoic acids effectively modulate stress-responsive transcriptional networks. Exogenous application of various fatty acids to wild-type and JA-deficient mutants confirmed AA as the signaling molecule. Moreover, AA treatment elicited heightened expression of general stress-responsive genes. Importantly, tomato (Solanum lycopersicum) leaves treated with AA exhibited reduced susceptibility to Botrytis cinerea infection, confirming AA signaling in other plants. These studies support the role of AA, an ancient metazoan signaling molecule, in eliciting plant stress and defense signaling networks.

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“…Studies of purification, characterization, and gene expression were performed; however, its biological function is still little known [2].…”

Section: Introductionmentioning

confidence: 99%

Extraction of Ascorbate Oxidase from Cucurbita maxima by Continuous Process in Perforated Rotating Disc Contactor Using Aqueous Two-Phase Systems

Porto

Marques

Porto

et al. 2009

Appl Biochem Biotechnol

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The ascorbate oxidase is the enzyme used to determine the content of ascorbic acid in the pharmaceutical and food industries and clinics analyses. The techniques currently used for the purification of this enzyme raise its production cost. Thus, the development of alternative processes and with the potential to reduce costs is interesting. The application of aqueous two-phase system is proposed as an alternative to purification because it enables good separation of biomolecules. The objective of this study was to determine the conditions to continuously pre-purify the enzyme ascorbate oxidase by an aqueous two-phase system (PEG/citrate) using rotating column provided with perforated discs. Under the best conditions (20,000 g/mol PEG molar mass, 10% PEG concentration, and 25% citrate concentration), the system showed satisfactory results (partition coefficient, 3.35; separation efficiency, 54.98%; and purification factor, 1.46) and proved suitable for the pre-purification of ascorbate oxidase in continuous process.

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Gene expression and enzyme activity of pepper (Capsicum annuum L.) ascorbate oxidase during elicitor and wounding stress

Cited by 31 publications

References 37 publications

Antioxidant content and ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation

Antioxidant content and ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation

Arachidonic Acid: An Evolutionarily Conserved Signaling Molecule Modulates Plant Stress Signaling Networks

Extraction of Ascorbate Oxidase from Cucurbita maxima by Continuous Process in Perforated Rotating Disc Contactor Using Aqueous Two-Phase Systems

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