Gene editing and genetic engineering approaches for advanced probiotics: A review

Yadav, Rajkumar; Kumar, Vishal; Baweja, Mehak; Shukla, Pratyoosh

doi:10.1080/10408398.2016.1274877

Cited by 81 publications

(45 citation statements)

References 115 publications

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“…Indeed, other legislation must be put in place in the near future for harmful levels of BAs in fermented dairy products, given that at present no upper limit for potentially toxic levels of histamine and tyramine are available. The search for probiotics is on-going using both genetic and traditional screening methods 61, 62 . Probiotics have a bright future in the area of supplemented fermented foods for health promotion.…”

Section: Resultsmentioning

confidence: 99%

Recent advances in microbial fermentation for dairy and health

et al. 2017

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Microbial fermentation has been used historically for the preservation of foods, the health benefits of which have since come to light. Early dairy fermentations depended on the spontaneous activity of the indigenous microbiota of the milk. Modern fermentations rely on defined starter cultures with desirable characteristics to ensure consistency and commercial viability. The selection of defined starters depends on specific phenotypes that benefit the product by guaranteeing shelf life and ensuring safety, texture, and flavour. Lactic acid bacteria can produce a number of bioactive metabolites during fermentation, such as bacteriocins, biogenic amines, exopolysaccharides, and proteolytically released peptides, among others. Prebiotics are added to food fermentations to improve the performance of probiotics. It has also been found that prebiotics fermented in the gut can have benefits that go beyond helping probiotic growth. Studies are now looking at how the fermentation of prebiotics such as fructo-oligosaccharides can help in the prevention of diseases such as osteoporosis, obesity, and colorectal cancer. The potential to prevent or even treat disease through the fermentation of food is a medically and commercially attractive goal and is showing increasing promise. However, the stringent regulation of probiotics is beginning to detrimentally affect the field and limit their application.

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Section: Resultsmentioning

confidence: 99%

Recent advances in microbial fermentation for dairy and health

et al. 2017

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“…The CRISPR–Cas immune system is an RNA‐mediated, DNA‐encoded, DNA‐targeting defense mechanism. In this, DNA is targeted by the sequence‐specific method and this help in providing the next generation of probiotics . Multiple CRISPR families have been identified within Lactobacillus , Streptococcus , and Bifidobacterium .…”

Section: Synthetic Biology For the Study Of Host–microbe Interactionsmentioning

confidence: 99%

“…It also provides a beneficial practice to cure metabolic disorders. Such disorders are costly to treat as they require strict acquiescence affecting lifestyle and survival rates of the patient; therefore, engineered microbes play a critical role in curing metabolic disorders . The engineered microbes that survive in the gastrointestinal tract of the patient while treating and alleviating disease symptoms show long‐lasting benefits.…”

Section: Introductionmentioning

confidence: 99%

Efficient engineered probiotics using synthetic biology approaches: A review

Yadav

Shukla

2019

Biotech and App Biochem

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The uses of probiotics‐based food supplements are getting emphasis due to their power to ensure better health conditions. Probiotics have diverse and significant applications in the health sector, so probiotic strains require an understanding of the genome level organizations. Probiotics elucidate various functional parameters that control their metabolic functions. In this review, we have compiled aspects of synthetic biology, which are used for the optimization of metabolic processes in probiotics for their use as a supplement in allopathic medicines. Synthetic biology approaches provide information about diverse biosynthetic pathways and also facilitate the novel metabolic engineering approaches for probiotics strain improvement. We have discussed the synthetic biology approaches for producing engineered probiotics via genetic circuits, expression systems, and genome editing tools like CRISPR–Cas and PEVLAB. This review also enlightens future challenges in the development of engineered probiotics.

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“…All these improvements enable the researchers to select the next‐generation probiotics more efficiently, either from the traditional sources such as intestinal content and dairy food or from the unconventional sources such as fermented foods, grain, vegetables, fruit, honey‐comb, and soil . Moreover, the genetically modified microorganisms may also be included in the next‐generation probiotics, despite their use still has ethical concerns and the safety problem needs to be solved . Together, the next‐generation probiotics, which have no history of use, can be discovered by novel molecular biology approaches or created by gene editing (Figure.…”

Section: Microbiome‐based Anti‐obesity Potential: Next‐generation Promentioning

confidence: 99%

“…38 Moreover, the genetically modified microorganisms may also be included in the next-generation probiotics, despite their use still has ethical concerns and the safety problem needs to be solved. 26,40 Together, the next-generation probiotics, which have no history of use, can be discovered by novel molecular biology approaches or created by gene editing (Figure. 2). However, it is notable that the term "next-generation probiotics" is relative and contemporary.…”

Section: Discovery Of the Next-generation Probioticsmentioning

confidence: 99%

An overview of microbiome based strategies on anti‐obesity

Chang

Ruan

Kao

2019

The Kaohsiung J of Med Scie

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With the significant global obesity epidemic and emerging strong scientific evidence that connected gut microbiota to obesity, intervening obesity by targeting gut microbiota has become a trendy strategy. Particularly the application of probiotics has become remarkably popular because of their expected association with gut microbiota modulation. Although there are many literatures on the effects of probiotics in obese animal models, most of them reported the effects of probiotic bacteria on metabolic indications with limited information on anti‐obesity itself. Besides, some probiotics have been shown to reduce certain metabolic symptoms but they failed to achieve weight loss. This report reviewed the current literatures on the anti‐obesity effects of next‐generation probiotics in various animal obesity models and discussed the beneficial potential of fecal microbiota transplantation in treating obesity in humans. The purpose of this article is to help guide further research improve the probiotic bacteria experiments in more precise animal obesity models by standardizing the anti‐obesogenesis, obesity control, and treatment assays and hopefully the evidence‐based investigations on harnessing gut microbiota through next‐generation probiotics or fecal microbiota transplantation will develop new interventions to promote and achieve anti‐obesity.

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Gene editing and genetic engineering approaches for advanced probiotics: A review

Cited by 81 publications

References 115 publications

Recent advances in microbial fermentation for dairy and health

Recent advances in microbial fermentation for dairy and health

Efficient engineered probiotics using synthetic biology approaches: A review

An overview of microbiome based strategies on anti‐obesity

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