Efficient engineered probiotics using synthetic biology approaches: A review

Abstract

The uses of probiotics‐based food supplements are getting emphasis due to their power to ensure better health conditions. Probiotics have diverse and significant applications in the health sector, so probiotic strains require an understanding of the genome level organizations. Probiotics elucidate various functional parameters that control their metabolic functions. In this review, we have compiled aspects of synthetic biology, which are used for the optimization of metabolic processes in probiotics for their use as a supplement in allopathic medicines. Synthetic biology approaches provide information about diverse biosynthetic pathways and also facilitate the novel metabolic engineering approaches for probiotics strain improvement. We have discussed the synthetic biology approaches for producing engineered probiotics via genetic circuits, expression systems, and genome editing tools like CRISPR–Cas and PEVLAB. This review also enlightens future challenges in the development of engineered probiotics.

Engineered probiotics

“…These gene-editing tools introduce Double Strand Break (DSB) into target genes, DNA Repair is induced by an error-prone Non-homologous End Joining (NHEJ) pathway or Homology Directed Repair (HDR) [ 31 ]. ZFNs and TALEN are two artificial restriction endonucleases which use zincfinger DNA domains or TAL-effect DNA domains to edit or cut specific target DNA [ 32 ].…”

The Inoculation of Probiotics In Vivo Is a Challenge: Strategies to Improve Their Survival, to Avoid Unpleasant Changes, or to Enhance Their Performances in Beverages

“…acidophilus Reduction of attachment of Escherichia coli ETEC to porcine intestinal brush border [68] Lb. gasseri Alleviation of diabetes Mellitus in rat model [93] Lb A treatment at 100 W for 2 and 3 min also enhanced (p < 0.05) intracellular and extracellular β-glucosidase activity of probiotics, leading to increased (p < 0.05) bioconversion of glucosides to aglycones in the prebiotic-soymilk.…”

Using Probiotics to Mute Salmonella enteric Serovar Typhimurium: An Opinion

“…Along with the rapid development of synthetic biology, interest has increased on the design and construction of GM probiotics as live biotherapeutics for a range of medical applications (Chua et al, 2017;Mays and Nair, 2018;Aggarwal et al, 2020). Synthetic biology explores diverse biosynthetic pathways and provides versatile engineering toolboxes for probiotic strain improvement (Yadav and Shukla, 2020). These toolboxes, including genetic circuits, different delivery systems, and a large number of genome-editing tools, successfully accelerated the development of advanced GM probiotics (Bober et al, 2018;Ozdemir et al, 2018).…”